Impact of electron-electron interaction on the electron energy distribution function in molecular plasmas under rf field action

Calculations of the electron energy distribution and of relevant macroscopic quantities of collision-dominated, weakly ionized plasmas under rf field action have been performed with increasing degrees of ionization, and the impact of the electron-electron interaction on these quantities was determined. The investigations were performed for the gas plasmas in CO and H₂ as representatives of molecular plasmas The energy distribution and macroscopic quantities are obtained by solving the nonstationary Bolizmann equation for a given rf field and degree of ionization taking into accoung and additional Fokker-Planck term besides the collision integrals for the elastic and the main inelastic collision processes. In these molecular plasmas a remarkable impact of the electron-electron interaction connected with increasing Maxwellization is observed for degrees of ionization greater than 10.     

Researches on pyridodiazepines. Behavior of 7,8,9,10-tetrahydro-5-methylcyclopenta[e]pyrido[3,2-b][1,4]-diazepin-6(5H)-one with chloroacetyl chloride

Following a previous experiment, an original heteropolycyclic structure 4 was obtained by a reaction of chloroacetyl chloride with compound 3 bearing a conjugated double bond system. The condensation develops with an initial NH-chloroacetylation and ring closure by quaternarization of the pyridine nitrogen. This is achieved through an 1,4 -cycloaddition of chloroketene to make a pyranone ring.     

Nonequilibrium vibrational populations of diatomic species in electrical discharges: Effects on the dissociation rates

Dissociation rates of N₂ in N₂-H₂ mixtures and of H₂ in Ar-H₂ mixtures have been calculated for conditions typical of gas discharges. The results have been obtained by solving a system of master equations including V-V (vibration-vibration), V-T (vibration-translation) and e-V (electron-vibration) energy exchanges. The results show that these rates are much smaller than the corresponding ones for pure gases, as a consequence of the increased importance of V-T processes. A simplified model, which takes into account the main features of the distribution of vibrational levels in an electrical discharge, has been built up to rationalize the present results as well as those derived in our previous work.     

Hemithio-analogues of meisenheimer complexes and sulphide group activation of aromatic substitution by alkoxides

EtO^? in DMSO adds to ethylthiopicrate at C-3 to generate an ephemeral

-adduct which ends into 1-ethylthio-4-ethoxy-2,5-dinitrobenzene, whilst ethyl picrate gives two adducts of attack of EtS^? at C-3 or C-1.     

PHOTOMORPHOGENIC RESPONSES TO UV RADIATION. II: A COMPARATIVE STUDY OF UV EFFECTS ON HYPOCOTYL ELONGATION IN A WILD-TYPE AND AN aurea MUTANT OF TOMATO (Lycopersicon esculentum Mill.)

Hypocotyl growth in etiolated seedlings of wild-type and an aurea mutant of tomato (Lycopersicon esculenturn Mill.), that appears to be deficient in labile phytochrome, is strongly inhibited by UV radiation in the region of 300–400 nm. The role of phytochrome in the UV-mediated inhibition of hypocotyl growth was studied using different experimental approaches: (1) by comparing the effectiveness of treatments of increasing duration of exposure to 692 nm and UV radiation; (2) by modifying the UV spectral range with specific cut-off filters. The experimental results suggest that the UV-induced inhibition of growth in wild-type tomato is mediated to a large extent by the longer wavelengths of the UV-A region and is mediated mainly by phytochrome. In contrast, at wavelengths < 305 nm a strong UV-B effect was found in the aurea mutant, suggesting a preeminent action of a specific UV-B absorbing photoreceptor that displays less action in the wild-type.     

Reversible immobilization of engineered molecules by Ni-NTA chelators

Electrochemical synthesis of nickel-nitrilotriacetic acid (Ni-NTA) chelators, for subsequent immobilization of (His)(6)-tagged proteins (Photosystem II (PSII) as model molecule), on Au or Au-graphite electrodes is compared to chemical synthesis. Results show: (i) higher Ni-NTA surface density, (ii) shorter treatment time (1-12 min vs. 16 h normally needed for self-assembled monolayer (SAM)), (iii) possibility of addressing the chelator to only one Au electrode, in a sensor micro-array.     

Peroxidase based biosensors for the selective determination of D,L-lactic acid and L-malic acid in wines

A novel bioelectrochemical method for the direct determination of D(−) L(+) lactic acid and of L(−) malic acid in wines is presented. Multienzymatic biosensors were realized for the selective determination of the three analytes: D(−) and L(+) lactic acid were measured by a trienzymatic biosensor based on the catalytic activities of the enzymes L(+) lactate oxidase (LOD), D(−) lactate dehydrogenase (D-LDH) and horseradish peroxidase (HRP); L(−) malic acid was measured by a bienzymatic electrode, realized by coupling the enzymes L(−) malic dehydrogenase (L-MDH) and horseradish peroxidase (HRP). In both cases the enzymes were immobilized on an oxygen selective Clark electrode.The simultaneous determination of the two organic acids can be accomplished either in batch or in a flow injection analysis apparatus using the same biosensors as detectors. The analytical performance of the method, tested in standard aqueous solutions and on real samples of wines, showing high repeatability, short response times and reduced cost of analysis, suggest that the experimental approach here described could be followed to monitor the progress of malolactic fermentation.     

Radical anions of nitrobenzothiazoles: EPR study of conjugative properties of benzothiazolyl systems

The electrochemical reduction in DMSO of the five isomers of nitrobenzothiazole (NBTZ) gave quite persistent radical anions that could be easily characterised by EPR spectroscopy. By contrast, the chemical reduction in alkaline solution, that is by t-BuOK in DMSO or by glucose and MeOK in MeOH, presented some problems with 6- and 4-NBTZ, and in the case of 2-NBTZ did not provide any detectable paramagnetic species. The internal consistency of coupling constants of the nitrobenzothiazole radical anions is in good agreement with the conjugative properties of the various benzothiazolyl systems and allows rectifying a recent EPR characterisation of 6-NBTZ radical anion.