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991.
Primiceri E Chiriacò MS Dioguardi F Monteduro AG D'Amone E Rinaldi R Giannelli G Maruccio G 《Lab on a chip》2011,11(23):4081-4086
Here an EIS (electrochemical impedance spectroscopy) biochip to detect cell migration is demonstrated. This biochip has been inspired by a traditional transwell assay/modified Boyden chamber and consists of two compartments separated by a porous membrane. This structure (PDMS-based) is aligned to EIS sensors. Cells are seeded in the upper chamber through microfluidic channels. During migration cells go through the pores of the membrane and get in touch with the electrodes that detect migrated cells. The performance of our cell-chip was tested by investigating the migratory ability of hepatocellular carcinoma (HCC) cells as a function of microenvironment. For this purpose we challenged HCC cells to migrate on different extra-cellular matrix (ECM) components including laminin 1, collagen IV and laminin 5. The results reveal that our cell chip provides reliable results that consistently overlap with those obtained with traditional standardized Boyden chambers. Thus, we demonstrate a new, easy tool to study cell migration and to perform automatic assays. This approach is easier and faster than traditional transwell assays and can be suitable for high-throughput studies in drug discovery applications. 相似文献
992.
The mechanism of the reaction between carbodiimides and activated α,β-unsaturated carboxylic acids yielding fully substituted hydantoins and variable amounts of N-acyl urea by-products was studied using density functional theory calculations. Two alternative pathways featuring N-acyl ureas and imino-oxazolidinones as intermediates for the formation of the hydantoin product were taken into account. The results obtained using two different computational models indicate that the overall barriers are similar for the two pathways considered. In all cases, inclusion of a second molecule of carboxylic acid was required to mediate tautomerizations and rearrangement steps. The calculations successfully reproduce the experimentally observed regioselectivity with respect to both N-acyl urea and hydantoin products. 相似文献
993.
Tuccinardi T Zizzari AT Brullo C Daniele S Musumeci F Schenone S Trincavelli ML Martini C Martinelli A Giorgi G Botta M 《Organic & biomolecular chemistry》2011,9(12):4448-4455
A(1) adenosine receptor antagonists have been proposed to possess an interesting range of potential therapeutic applications. We have already reported the synthesis and the biological characterization of a family of pyrazolo[3,4-b]pyridine derivatives as A(1) adenosine ligands endowed with an antagonistic profile. In the present work, we report the LC separation of enantiomers of our most active A(1) antagonists together with the determination of their absolute configuration by means of X-ray crystal structure analysis. Biological assays confirmed a different activity for the two enantiomers, with the R one showing the higher human A(1)AR affinity. We also developed a homology model of this receptor subtype in order to suggest a binding disposition of the ligands into the hA(1)AR. All of the obtained data suggest that the compound's chirality plays a key role in A(1) affinity. 相似文献
994.
Toja F Nevin A Comelli D Levi M Cubeddu R Toniolo L 《Analytical and bioanalytical chemistry》2011,399(9):2977-2986
The preservation of design object collections requires an understanding of their constituent materials which are often polymeric
blends. Challenges associated with aging of complex polymers from objects with an unknown physical history may compromise
the interpretation of data from analytical techniques, and therefore complicate the assessment of the condition of polymers
in indoor museum environments. This study focuses on the analysis of polymeric materials from three well-known Italian design
lamps from the 1960s. To assess the degree of chemical modifications in the polymers, non-destructive molecular spectroscopic
techniques, Fourier-transform infrared (FTIR) and fluorescence spectroscopy, have been applied directly on the object surfaces
using an optical fiber probe and through examination of micro samples. FTIR spectra of the different polymers, polyvinylacetate
(PVAc) for the lamps Taraxacum and Fantasma, and both acrylonitrile–butadiene–styrene polymer (ABS) and cellulose acetate
(CA) for the lamp Nesso, allowed the detection of ongoing deterioration processes. Fluorescence spectroscopy proved particularly
sensitive for the detection of molecular changes in the polymeric objects, as the spectra obtained from the examined lamps
differ significantly from those of the unaged reference materials. Differences in fluorescence spectra are also detected between
different points on the same object further indicating the presence of different chemical species on the surfaces. With the
aid of complementary data from FTIR spectroscopy, an interpretation of the emission spectra of the studied polymeric objects
is here proposed, further suggesting that fluorescence spectroscopy may be useful for following the degradation of historical
polymeric objects. 相似文献
995.
A multi-analytical approach for the characterization of powders from the Pompeii archaeological site
Canevali C Gentile P Orlandi M Modugno F Lucejko JJ Colombini MP Brambilla L Goidanich S Riedo C Chiantore O Baraldi P Baraldi C Gamberini MC 《Analytical and bioanalytical chemistry》2011,401(6):1801-1814
Nine black powders found in Pompeii houses in three different types of bronze vessels (cylindrical theca atramentaria, unguentaries, and aryballoi) were characterized in order to assess a correspondence between the composition and the type of vessel and, possibly, to verify if these powders were inks or not. For the compositional characterization, a multi-analytical approach was adopted, which involved the use of scanning electron microscopy-energy dispersive X-ray, Fourier-transformed infrared spectroscopy, Raman, X-ray diffraction, electron paramagnetic resonance spectroscopy, thermogravimetric analysis, gas chromatography coupled with mass spectrometry (GC/MS), and pyrolysis GC/MS. Powders contained in cylindrical theca atramentaria form a homogeneous group, and their organic and inorganic compositions suggest that they were writing inks, while powders contained in unguentaries and aryballoi could have had several different uses, including writing inks and cosmetics. Furthermore, the composition profile of the powders found in cylindrical cases shows that, at 79?AD: , in Pompeii, carbon-based inks were still used for writing, and iron gall inks had not been introduced yet. 相似文献
996.
Cheng HM Leigh DA Maffei F McGonigal PR Slawin AM Wu J 《Journal of the American Chemical Society》2011,133(31):12298-12303
We report that a 2,2':6',2″-terpyridylmacrocycle-Ni complex can efficiently mediate the threading of two alkyl chains with bulky end groups in an active metal template sp(3)-carbon-to-sp(3)-carbon homocoupling reaction, resulting in a rare example of a doubly threaded [3]rotaxane in up to 51% yield. The unusual architecture is confirmed by X-ray crystallography (the first time that a one-ring-two-thread [3]rotaxane has been characterized in the solid state) and is found to be stable with respect to dethreading despite the large ring size of the macrocycle. Through such active template reactions, in principle, a macrocycle should be able to assemble as many axles in its cavity as the size of the ring and the stoppers will allow. A general method for threading multiple axles through a macrocycle adds significantly to the tools available for the synthesis of different types of rotaxane architectures. 相似文献
997.
Banci L Bertini I Blaževitš O Cantini F Lelli M Luchinat C Mao J Vieru M 《Journal of the American Chemical Society》2011,133(2):345-349
Demetalated superoxide dismutase (SOD1) is a transient species, fibrillogenic in nature and of biomedical interest. It is a conformationally disordered protein difficult to characterize. We have developed a strategy based on the NMR investigation of a crystalline species characterized by X-ray crystallography and on the comparison of the solid-state-solution-state chemical shifts. The solid-state assignment has been also helpful in assigning the solution spectra. The solution NMR spectra presumably detect species that are the result of equilibria among multiple species. From the differences in chemical shifts between the two forms, we learned that a β-sheet becomes conformationally labile and two loops in the same sheet show propensity to take a β conformation. This strategy, which exploits solution and solid-state NMR spectra in a synergistic way, thus provides information on the species that are prone to oligomerize. 相似文献
998.
Despite the growing importance of qualitative screening tests in routine laboratories involved in the EU official control, their validation is not as deeply explained in Commission Decision 2002/657/EC as the validation of quantitative confirmatory methods. At the same time, the issue of quality assurance of screening assays defining internal quality control (IQC) procedures as required by accreditation bodies is undoubtedly less developed in this analytical field. As an example the present study describes the development, the validation and the IQC implemented for a commercial enzyme linked immunosorbent assay (ELISA) able to detect 17-α-19-nortestosterone (α-NT) and 17-β-19-nortestosterone (β-NT) isomers in bullock urine. In order to select a suitable sample treatment, two SPE purification protocols were preliminary compared. The chosen method was therefore fully validated determining the mandatory parameters required by Commission Decision 2002/657/EC: specificity, detection capability and robustness. An in-depth discussion was carried out illustrating the possible validation approaches and their implications especially in the assessment of the key performance characteristic: detection capability. Finally, the control charts implemented for continuous method verification during analyses of real samples were reported. 相似文献
999.
1000.
D'Anna F Frenna V Ghelfi F Marullo S Spinelli D 《The Journal of organic chemistry》2011,76(8):2672-2679
The reaction rates for the rearrangement of eleven (Z)-arylhydrazones of 5-amino-3-benzoyl-1,2,4-oxadiazole 3a-k into the relevant (2-aryl-5-phenyl-2H-1,2,3-triazol-4-yl)ureas 4a-k in the presence of trichloroacetic acid or of piperidine have been determined in toluene at 313.1 K. The results have been related to the effect of the aryl substituent by using Hammett and/or Ingold-Yukawa-Tsuno correlations and have been compared with those previously collected in a protic polar solvent (dioxane/water) as well as with those on the analogous rearrangement of the corresponding (Z)-arylhydrazones of 3-benzoyl-5-phenyl-1,2,4-oxadiazole 1a-k in benzene. Some light can thus be shed on the general differences of chemical reactivity between protic polar (or dipolar aprotic) and apolar solvents. 相似文献