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Abrunhosa F Faria S Gomes P Tomaz I Pessoa JC Andreu D Bastos M 《The journal of physical chemistry. B》2005,109(36):17311-17319
The interaction of two hybrid peptides of cecropin A and melittin [CA(1-8)M(1-18) and CA(1-7)M(2-9)] with liposomes was studied by differential scanning calorimetry (DSC), circular dichroism (CD), and quasi-elastic light scattering (QELS). The study was carried out with large unilamellar vesicles (LUVs) of three different lipid compositions: 1,2-dimyristoil-sn-glycero-3-phosphocholine (DMPC), 1,2-dimyristoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DMPG) and a binary mixture of DMPC/DMPG, in a wide range of peptide-to-lipid (P:L) molar ratios (0 to 1:7). DSC results indicate that, for both peptides, the interaction depends on membrane composition, with very different behavior for zwitterionic and anionic membranes. CD data show that, although the two peptides have different secondary structures in buffer (random coil for CA(1-7)M(2-9) and predominantly beta-sheet for CA(1-8)M(1-18)), they both adopt an alpha-helical structure in the presence of the membranes. Overall, results are compatible with a model involving a strong electrostatic surface interaction between the peptides and the negatively charged liposomes, which gives place to aggregation in the gel phase and precipitation after a threshold peptide concentration. In the case of zwitterionic membranes, a progressive surface coverage with peptide molecules destabilizes the membrane, eventually leading to membrane disruption. Moreover, delicate modulations in behavior were observed depending on the peptide. 相似文献
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Maxime Guitet Pinglu Zhang Filipa Marcelo Coralie Tugny Jesús Jimnez‐Barbero Olivier Buriez Christian Amatore Virginie Mouris‐Mansuy Jean‐Philippe Goddard Louis Fensterbank Yongmin Zhang Sylvain Roland Mickaël Mnand Matthieu Sollogoub 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2013,125(28):7354-7359
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Activated carbon with high surface area was prepared from sodium salt of carboxyl methyl cellulose by physical activation at 400 °C followed by treatment in boiling water. The carbon was characterized by XRD, SEM, EPR, TG, IR, surface areas and porosity measurements. The carbon showed alkaline reaction in aqueous solution. It showed high surface area and pore volume in comparison to two commercially available carbons. 相似文献
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