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961.
A foodborne illness caused by type A toxin-producing Clostridium botulinum was investigated by using the standard mouse bioassay and a rapid invitro test for toxin detection. The patient, who consumed improperly stored hash brown potatoes that contained the preformed toxin, was diagnosed with type A botulism. C. botulinum type A toxin was detected in the hash brown potatoes as well as in the tryptone-peptone-glucose-yeast extract (TPGY) medium subcultures of this food using the mouse bioassay and an amplified ELISA technique. The mouse bioassay revealed preformed toxin at 10,000 minimum lethal dose (MLD)/g uncooked product and the amplified ELISA an equivalent 50,000 MLD/g. The cultural toxin from the uncooked product killed mice at the 10(6) dilution and a modification of the ELISA procedure was positive at the 10(3) dilution. Cooked food obtained from the consumer's waste can contained 100 MLD/g and the ELISA was also positive at the same dilution of the product. The culture of the cooked product obtained from the waste can was lethal for mice at the 10(7) dilution and positive using the modified ELISA at the 10(4) dilution. The unmodified amplified ELISA method indicated a toxin level of approximately 1 ng/mL (equivalent to 5 x 10(5) MLD/mL) in diluted culture fluid from the uncooked food and the culture of cooked food obtained from the waste can. The hash brown potatoes were negative for types B, E, and F preformed and cultural botulinal toxins using both assays. 相似文献
962.
963.
Lucas C. F. Ferreira Marcelo Montenegro Matheus C. Santos 《Journal d'Analyse Mathématique》2016,128(1):1-49
We define the Polish space R of non-degenerate rank-1 systems. Each non-degenerate rank-1 system can be viewed as a measure-preserving transformation of an atomless, σ-finite measure space and as a homeomorphism of a Cantor space. We completely characterize when two non-degenerate rank-1 systems are topologically isomorphic. We also analyze the complexity of the topological isomorphism relation on R, showing that it is \({F_\sigma }\) as a subset of R× R and bi-reducible to E0. We also explicitly describe when a non-degenerate rank-1 system is topologically isomorphic to its inverse. 相似文献
964.
L. N. Jorge L. S. Ferreira S. A. Leão A. A. Caparica 《Brazilian Journal of Physics》2016,46(5):556-564
In this work, we use a refined entropic sampling technique based on the Wang-Landau method to study the spin- 1/2 Baxter-Wu model. We adopt the total magnetization as the order parameter and, as a result, do not divide the system into three sub-lattices. The static critical exponents were determined as α = 0.6697(54), β = 0.0813(67), γ = 1.1772(33), and ν = 0.6574(61). The estimate for the critical temperature was Tc = 2.26924(2). We compare the present results with those obtained from other well-established approaches, and we find a very good closeness with the exact values, besides the high precision reached for the critical temperature. 相似文献
965.
Reggiani Vilela Gonçalves Rômulo Dias Novaes Mariáurea Matias Sarandy João Paulo Viana Leite Emerson Ferreira Vilela Marli do Carmo Cupertino 《Natural product research》2016,30(23):2738-2742
The aim of this study was to investigate the effect of leaves extract of Schizocalyx cuspidatus (A. St.-Hil.) Kainul. & B. Bremer on hepatic morphofunctional dysfunction induced by carbon tetrachloride (CCl4). Liver lesions were induced via intraperitoneal administration of CCl4 every 48 h for 12 days. Forty-nine rats were randomised into seven groups: G1: CCl4; G2: CCl4 (animals euthanised 24 h after last CCl4 application); G3: CCl4 + DMSO; G4: SCE 400 mg/kg; G5: DMSO (700 μl); G6: CCl4 + SCE 200 mg/kg and G7: CCl4 + SCE 400 mg/kg. SCE administration resulted in reduction in hydroperoxide levels, lipidic droplets and necrosis compared to G1, G2 and G3. There was an increase in the amount of collagen fibres in G1, G2 and G3 compared to the groups. These results show that the extract of SCE leaves has great potential for the recovery of liver damage after the application of CCl4. 相似文献
966.
In this paper we study eigenfunctions and fundamental solutions for the three parameter fractional Laplace operator \(\Delta _+^{(\alpha , \beta , \gamma )}:= D_{x_0^+}^{1+\alpha } +D_{y_0^+}^{1+\beta } +D_{z_0^+}^{1+\gamma },\) where \((\alpha , \beta , \gamma ) \in \,]0,1]^3\), and the fractional derivatives \(D_{x_0^+}^{1+\alpha }, D_{y_0^+}^{1+\beta }, D_{z_0^+}^{1+\gamma }\) are in the Riemann–Liouville sense. Applying operational techniques via two-dimensional Laplace transform we describe a complete family of eigenfunctions and fundamental solutions of the operator \(\Delta _+^{(\alpha ,\beta ,\gamma )}\) in classes of functions admitting a summable fractional derivative. Making use of the Mittag–Leffler function, a symbolic operational form of the solutions is presented. From the obtained family of fundamental solutions we deduce a family of fundamental solutions of the fractional Dirac operator, which factorizes the fractional Laplace operator. We apply also the method of separation of variables to obtain eigenfunctions and fundamental solutions. 相似文献
967.
Four artemisinin reductive decomposition routes A, B1, B2, and B3 with 13 species (QHS, 1/2, 3, 4, 5, 5a, 6, 7, 18, 18a, 19, 20, and 21) were studied at the B3LYP/6-31G** level. Structures of the species were analyzed in terms of geometrical parameters, L?wdin bond orders, partial atomic charges and spin densities, electronic and free energies, and entropy. Searches in the Cambridge Structural Database for high-level quality artemisinin-related structures were also performed. Principal Component and Hierarchical Cluster analyses were performed on selected electronic and structural variables to rationalize relationships between the routes. The A and B1 routes are possibly interconnected. Structural and electronic features of all species show that there are two clusters: A-B1 and B2-B3. The latter cluster is thermodynamically more favorable (DeltaDeltaG is -64 to -88 kcal mol(-1)) than the former (DeltaDeltaG is -58 to -59 kcal mol(-1)), but kinetical preference may be the opposite. Along the artemisinin decomposition routes, especially B2 and B3, larger structural changes including formation of branched structures and CO2 release are related to increased exothermicity of the conversions, weakened attractive oxygen-oxygen interactions, and increased entropy of the formed species. The intermediate 4 definitely belongs to some minor artemisinin decomposition route. 相似文献
968.
969.
Fbio M. Amorin Natali Ademir Pastor Ferreira 《Journal of Mathematical Analysis and Applications》2008,347(2):428-441
In the present paper we show some results concerning the orbital stability of dnoidal standing wave solutions and orbital instability of cnoidal standing wave solutions to the following Klein–Gordon equation:
utt−uxx+u−|u|2u=0.