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排序方式: 共有165条查询结果,搜索用时 31 毫秒
91.
We have developed diffraction phase microscopy as a new technique for quantitative phase imaging of biological structures. The method combines the principles of common path interferometry and single-shot phase imaging and is characterized by subnanometer path-length stability and millisecond-scale acquisition time. The potential of the technique for quantifying nanoscale motions in live cells is demonstrated by experiments on red blood cells. 相似文献
92.
Popescu G Ikeda T Goda K Best-Popescu CA Laposata M Manley S Dasari RR Badizadegan K Feld MS 《Physical review letters》2006,97(21):218101
Using a novel noncontact technique based on optical interferometry, we quantify the nanoscale thermal fluctuations of red blood cells (RBCs) and giant unilamellar vesicles (GUVs). The measurements reveal a nonvanishing tension coefficient for RBCs, which increases as cells transition from a discocytic shape to a spherical shape. The tension coefficient measured for GUVs is, however, a factor of 4-24 smaller. By contrast, the bending moduli for cells and vesicles have similar values. This is consistent with the cytoskeleton confinement model, in which the cytoskeleton inhibits membrane fluctuations [Gov et al., Phys. Rev. Lett. 90, 228101, (2003). 相似文献
93.
Extended depth of focus in tomographic phase microscopy using a propagation algorithm 总被引:1,自引:0,他引:1
Tomographic phase microscopy is a laser interferometry technique in which a 3D refractive index map of a biological sample is constructed from quantitative phase images collected at a set of illumination angles. Although the resulting tomographic images provide valuable information, their resolution declines at axial distances beyond about 1 microm from the focal plane. We describe an improved 3D reconstruction algorithm in which the field at the focal plane is numerically propagated to depths throughout the sample. Diffraction is thus incorporated, extending the depth of focus to more than 10 mum. Tomograms with improved focal depth are demonstrated for single HT29 cells. 相似文献
94.
Ramachandra R. Dasari Gregory M. Shimkaveg Michael S. Otteson Michael S. Feld 《Hyperfine Interactions》1987,38(1-4):711-722
The ability of optical pumping to produce nuclear orientation and the advent of single frequency tunable dye lasers has brought
about a new interesting area of study, Laser Induced Nuclear Orientation (LINO). The impact of LINO on the nuclear structure
studies of short lived nuclei along with a discussion on various relaxation mechanisms involved are presented. As an example,
recent experimental results on85mRb (T1/2=1.0 μs) are given in detail. Possible application of LINO to151Eu discussed. 相似文献
95.
Ballistic light, i.e., radiation that propagates undeflected through a turbid medium, undergoes a small change in phase velocity and exhibits unusual dispersion because of its wave nature. We use a novel highly sensitive differential phase optical interferometer to study these previously unmeasurable phenomena. We find that ballistic propagation can be classified into three regimes based on the wavelength-to-size ratio. In the regime in which the scatterer size is comparable with the wavelength, there is an anomalous phase-velocity increase as a result of adding scatterers of higher refractive index. We also observe an anomaly in the relative phase velocity, where red light is slowed more than blue light even though the added scatterers are made of material with normal dispersion. 相似文献
96.
We report on phase-dispersion optical tomography, a new imaging technique based on phase measurements using low-coherence interferometry. The technique simultaneously probes the target with fundamental and second-harmonic light and interferometrically measures the relative phase shift of the backscattered light fields. This phase change can arise either from reflection at an interface within a sample or from bulk refraction. We show that this highly sensitive (~5 degrees ) phase technique can complement optical coherence tomography, which measures electric field amplitude, by revealing otherwise undetectable dispersive variations in the sample. 相似文献
97.
1-Benzyloxy-1,2,3-benzotriazole was photolyzed and found to yield benzaldehyde and azobenzene as the principal products. 相似文献
98.
We have used a novel phase-referenced heterodyne dual-beam low-coherence interferometer to perform what we believe are the first noncontact measurements of surface motion in a nerve bundle during the action potential. Nerve displacements of approximately 5-nm amplitude and approximately 10-ms duration are measured without signal averaging. This interferometer may find general application in measurement of small motion in cells and other weakly scattering samples. 相似文献
99.
The current status of the search for localized domains of disoriented chiral condensates (DCC) in Pb+Pb collisions at 158A GeV/c are presented. A new method based on the discrete wavelet transformation (DWT) technique along with an analysis of correlation between charged and neutral particles has been used for the search of localized DCC domains. We show that both the methods indicate the presence of non-statistical fluctuations in data. 相似文献
100.
Yang C Wax A Georgakoudi I Hanlon EB Badizadegan K Dasari RR Feld MS 《Optics letters》2000,25(20):1526-1528
We describe a new scanning microscopy technique, phase-dispersion microscopy (PDM). The technique is based on measuring the phase difference between the fundamental and the second-harmonic light in a novel interferometer. PDM is highly sensitive to subtle refractive-index differences that are due to dispersion (differential optical path sensitivity, 5 nm). We apply PDM to measure minute amounts of DNA in solution and to study biological tissue sections. We demonstrate that PDM performs better than conventional phase-contrast microscopy in imaging dispersive and weakly scattering samples. 相似文献