Comparison of crude extract from durio zibethinus M. (durian) leaf waste via ultrasound-assisted extraction and accelerated solvent extraction: antioxidant activity and cytotoxicity

Abstract

The objective of this study was to compare the antioxidant activity and cytotoxicity of Durio zibethinus M. (Durian) leaf extract from two extraction methods. Ultrasound-assisted extraction and Accelerated-solvent extraction were used to produce crude extract. The results revealed that UAE achieved 3× higher in total phenolic content in the leaf extract compared to ASE. DPPH radical scavenging activity was 4.6× higher in leaf extract from ASE. No significant differences reported in ferric reducing power, and total flavonoid content of the leaf extract between the two methods. Cytotoxicity via MTT assay demonstrated no significant differences in cell viability upon exposure to the leaf extract from both methods. This suggested that they were appropriate in producing Durio zibethinus M. leaf extract for end use application in food related product. Both ensured similar level of safety in Durio zibethinus M. leaf extract as a new potential ingredient for the food industry.     

Enantiospecific high-performance liquid chromatographic analysis of 2-phenylpropionic acid, ketoprofen and fenoprofen

A high-performance liquid chromatographic method has been developed for the quantitation of the R- and S-enantiomers of 2-phenylpropionic acid, ketoprofen and fenoprofen. The assay consists of extracting the arylpropionic acid with an internal standard and measuring the total (R + S) concentration of enantiomers by reversed-phase chromatography, derivatising the chromatographic fraction corresponding to the enantiomers to form R- and S, R-2-phenylethylamide distereoisomers which are resolved by normal-phase chromatography in order to calculate the fraction of each enantiomer. The limits of sensitivity of the assay for 2-phenylpropionic acid, ketoprofen and fenoprofen are 6, 0.2 and 2.5 mg/l, respectively.     

Structure property and mesomorphic behaviour of S-shaped liquid crystal oligomers possessing two azobenzene moieties

A new series of symmetrical S-shaped oligomers 4,4?-bis(ω-2-(ω-[(bromophenyl)diazenyl]-alkoxy)phenoxy)hexylbiphenyl consisting of two different spacers (inner -(CH₂)₆- and outer -(CH₂)_n-) have been synthesised. Their physical, thermal and texture observation over various transition temperatures are reported. The outer spacers for these compounds vary from n = 4 to n = 9. The oligomers with even number of members exhibit monotropic phase in which the compound with n = 4 shows nematic (N) phase whilst those with members n = 6 and 8 exhibit N and smectic A (SmA) phases. However, the homologs with odd number of members display enantiotropic phase in which the compound with n = 5 exhibits N and smectic phases whereas the members with n = 7 and 9 are predominantly smectogenic. The temperature range of N phase for even-numbered member decreased with elongation of the outer spacer. The smectic phase stability among the members in the present series increases when the outer spacer n is increased from 5 to 8.     

Covalent immobilization of invertase on PAMAM-dendrimer modified superparamagnetic iron oxide nanoparticles

In this study, polyamidoamine (PAMAM) dendrimer was synthesized on the surface of superparamagnetite nanoparticles to enhance invertase immobilization. The amount of immobilized enzyme on the surface-hyperbranched magnetite nanoparticle was up to 2.5 times (i.e., 250%) as much as that of magnetite nanoparticle modified with only amino silane. Maximum reaction rate (V _max) and Michaelis–Menten constant (K _m) were determined for the free and immobilized enzymes. Various characteristics of immobilized invertase such as; the temperature activity, thermal stability, operational stability, and storage stability were evaluated and results revealed that stability of the enzyme is improved upon immobilization.     

Duty-cycle division multiplexing (DCDM)

A multiplexing technique, which is based on duty-cycle division, is proposed. The channel multiplexing and demultiplexing are performed electrically at the single user bit rate, which is very economic. In a three-user system (3×10 Gb/s), the simulation results show that the best receiver sensitivity value achieved is −30.1 dBm with an optical signal-to-noise ratio (OSNR) of 22.3 dB, while the chromatic dispersion tolerance ranges from 192 to 280 ps/nm. Migration from 30 to 120 Gb/s is achieved with the penalty of 6.4 and 5.2 dB in the receiver sensitivity and OSNR, respectively, for the worst user.     

On the numerical solution of Sturm-Liouville equations in the theory of molecular diffusion

The Sturm-Liouville equation from Budo's Theory of diffusion in the presence of potential wells is solved numerically for the normalised complex polarisability across the complete range of well depth (Vo/kT) from zero to effectively infinite. For Vo/kT = 0 and Vo/kT = ∞ Debye process is recovered, in excellent agreement with available analytical limits. At intermediate Vo/kT the original Debye process for Vo/kT (free diffusion) is supplemented by a further loss process on the high frequency side. The numerical method used allow us to investigate the origin of this process in terms of the eigenvalues and eigenfunctions of the original Sturm-Liouville equation.     

Characterization of atenolol transformation products in ozonation by using rapid resolution high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry

This experiment investigated the transformation pathway of atenolol, a widely used β-blocker, in the ozonation process through the identification of generated intermediate compounds. In order to characterize the contribution of ozone and hydroxyl radical (•OH) in the transformation of atenolol, ozonation were performed at pH 2, 7 and 9. 15 major transformation products have been identified based on the chromatographic behavior of these compounds and the information obtained from accurate mass MS and MS/MS spectra. By comparing to the elution pattern of the identified transformation products and its fragmentation pattern in the MS/MS spectrum, a variety of isomers of the transformation products were characterized.Identified transformation products of atenolol are including its mono-, di and tri-hydroxylated derivatives as well as the aliphatic and aromatic ring breakdown products. Transformation of atenolol in the ozonation involved hydroxylation reaction, aromatic ring opening reaction, oxidation and cleavage of 2-hydroxy-3-(isopropylamino)propoxy group of atenolol. In ozonation, aromatic ring of atenolol was transformed through the reaction with both ozone and •OH whereas the aliphatic chain of atenolol was degraded mainly through the reaction with •OH. The results also indicated that both •OH and ozone involved in the aromatic ring opening reaction.     

Industrial noise levels and annoyance in Egypt

Annoyance and increase of accident risk of workers from industrial noise levels in Egypt were studied. 683 workers from 15 Egyptian sites of industry, ranging from food to metal industry were evaluated. The goals of this study are to carry out measurements to evaluate industrial noise levels, are these levels exceeded the permissible levels set by Egyptian noise standard and policy to protect public health of workers?, to examine worker’s attitudes towards industrial noise, to know the relationship between industrial noise levels and degree of annoyance. Results showed that equivalent continuous noise levels ranged from 70 to 100 dB (A). Annoyance of respondents showed that 47.1% were highly annoyed, 5.8% their hearing were harmed. There was a strong relationship between industrial noise levels and percentage of highly annoyed respondents. By increasing industrial noise level possibility of workers to make accident was also increased. Respondents suggest less maximum daily exposure duration than those set by Egyptian law.