Eun Seo Choi  Jihyeon Song  Yoon Young Kang  Hyejung Mok 《Macromolecular bioscience》2019,19(7)

The surface of bovine serum‐derived exosomes (EXOs) are modified with α‐d ‐mannose for facile interaction with mannose receptors on dendritic cells (DCs) and for efficient delivery of immune stimulators to the DCs. The surface of the EXOs is modified with polyethylene glycol (PEG) without particle aggregation (≈50 nm) via the incorporation of 1,2‐distearoyl‐sn‐glycero‐3‐phosphoethanolamine (DSPE) into the lipid layer of the EXO, compared to chemical conjugation by N‐hydroxysuccinimide activated PEG (NHS‐PEG). PEG modification onto the exosomal surface significantly decreases the non‐specific cellular uptake of the EXOs into the DCs. However, the EXOs with mannose‐conjugated PEG‐DSPE (EXO‐PEG‐man) exhibit excellent intracellular uptake into the DCs and boost the immune response by the incorporation of adjuvant, monophosphoryl lipid A (MPLA) within the EXO. After an intradermal injection, a higher retention of EXO‐PEG‐man is observed in the lymph nodes, which could be used for the efficient delivery of immune stimulators and antigens to the lymph nodes in vivo.  相似文献   

    

Inseon Kim  Seunghun S. Lee  Seung Hyun L. Kim  Sunghoon Bae  Hoyon Lee  Nathaniel S. Hwang 《Macromolecular bioscience》2019,19(5)

Bone is a vascularized tissue that is comprised of collagen fibers and calcium phosphate crystals such as hydroxyapatite (HAp) and whitlockite (WH). HAp and WH are known to elicit bone regeneration by stimulating osteoblast activities and osteogenic commitment of stem cells. In addition, vascular endothelial growth factor (VEGF) is shown to promote osteogenesis and angiogenesis which is considered as an essential process in bone repair by providing nutrients. In this study, VEGF‐secreting human adipose‐derived stem cells (VEGF‐ADSCs) are developed by transducing ADSCs with VEGF‐encoded lentivirus. Additionally, WH‐reinforced gelatin/heparin cryogels (WH‐C) are fabricated by loading WH into gelatin/heparin cryogels. VEGF‐ADSC secrete tenfold more VEGF than ADSC and show increased VEGF secretion with cell growth. Also, incorporation of WH into cryogels provides a mineralized environment with ions secreted from WH. When the VEGF‐ADSCs are seeded on WH‐C, sustained release of VEGF is observed due to the specific affinity of VEGF to heparin. Finally, the synergistic effect of VEGF‐ADSC and WH on osteogenesis is successfully confirmed by alkaline phosphatase and real‐time polymerase chain reaction analysis. In vivo bone formation is demonstrated via implantation of VEGF‐ADSC seeded WH‐C into mouse calvarial bone defect model, resulted in enhanced bone development with the highest bone volume/total volume.  相似文献   

    

Byunghyuck Jung  Jungkyu K. Lee  Jungnam Kim  Eunhye K. Kang  Sang Yeong Han  Hee‐Yoon Lee  Insung S. Choi 《化学:亚洲杂志》2019,14(21):3749-3762

(?)‐Cannabidiol ((?)‐CBD), a non‐psychoactive phytocannabinoid from Cannabis, and its structural analogs have received growing attention in recent years because of their potential therapeutic benefits, including neuroprotective, anti‐epileptic, anti‐inflammatory, anxiolytic, and anti‐cancer properties. (?)‐CBD and its analogs have been obtained mainly based on extraction from the natural source; however, the conventional extraction‐based methods have some drawbacks, such as poor quality control along with purification difficulty. Chemical‐synthetic strategies for (?)‐CBD could tackle these issues, and, additionally, generate novel (?)‐CBD analogs that exhibit advanced biological activities. This review concisely summarizes the historic and recent milestones in the synthetic strategies for (?)‐CBD and its analogs.  相似文献   

    

Ji‐Hye Jung  Aruna Kumar Mohanty  Jihwa Ye  Taeheon Lee  Junyoung Ahn  Yeong‐Gweon Lim  Taihyun Chang  Hyun‐Jong Paik 《Journal of polymer science. Part A, Polymer chemistry》2017,55(24):4020-4026

Covalent fixed multicyclic polystyrene conformers were synthesized from inconvertible polystyrene conformers based on p‐tert‐butylthiacalix[4]arene by azidation and subsequent click coupling. Selective 1D NOESY analyses confirmed that the spatial structures of star polystyrene precursors and multicyclic polystyrenes were preserved during the azidation and click coupling processes. The conformation of star polymers affected degree of decrease in the hydrodynamic volume after cyclization but had little effect on glass transition behavior. The rigid core of star polymer increased the T_g but in the case of multicyclic polymer, this influence of core structure was reduced. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017 , 55, 4020–4026  相似文献   

    

Deok‐Hwang Kwon  Seung Ran Lee  Yong Seok Choi  Seung‐Beom Son  Kyu Hwan Oh  Kookrin Char  Miyoung Kim 《固体物理学:研究快报》2017,11(5)

  相似文献   

    

Thanh Danh Nguyen  Min Seok Song  Nguyìn Hong Ly  So Yeong Lee  Sang‐Woo Joo 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(9):2736-2740

Multiple sharp‐edged gold nanostars were efficiently assembled on nanopipette tips through electrostatic interactions for use as a potent intracellular hypoxia‐sensing Raman probe. Colloidal stability and surface immobilization were checked using scanning electron microscopy, light scattering, and zeta potential measurements. Site‐specific intracellular hypoxia levels can be estimated in vitro and in vivo using Raman lancets (RL). Distinct Raman spectral changes for the nitro‐(NO₂) functional group of the redox marker 4‐nitrothiophenol (4NTP) can be quantified according to the intracellular oxygen (O₂) content, ranging from 1 % to 10 %. Redox potential changes in mitochondrial respiration were also examined through serial injections of inhibitors. 3D‐cultured cells and in vivo tests were used to validate our method, and its application in the assessment of the aggressiveness of cancer cells by differentiating spectral changes between malignant and benign cells was demonstrated.  相似文献   

    

Jeong Eun Yoo  Marco Altomare  Mohamed Mokhtar  Abdelmohsen Alshehri  Shaeel A. Al‐Thabaiti  Anca Mazare  Patrik Schmuki 《physica status solidi (a)》2016,213(10):2733-2740

  相似文献   

    

Jeongin Lee  Dr. Min-Ho Kim  Prof. Hosik Lee  Dr. Jonghak Kim  Jeongwoo Seo  Prof. Hyun-Wook Lee  Dr. Chihyun Hwang  Prof. Hyun-Kon Song 《Angewandte Chemie (International ed. in English)》2023,62(48):e202312928

High-capacity Li-rich layered oxides using oxygen redox as well as transition metal redox suffer from its structural instability due to lattice oxygen escaped from its structure during oxygen redox and the following electrolyte decomposition by the reactive oxygen species. Herein, we rescued a Li-rich layered oxide based on 4d transition metal by employing an organic superoxide dismutase mimics as a homogeneous electrolyte additive. Guaiacol scavenged superoxide radicals via dismutation or disproportionation to convert two superoxide molecules to peroxide and dioxygen after absorbing lithium superoxide on its partially negative oxygen of methoxy and hydroxyl groups. Additionally, guaiacol was decomposed to form a thin and stable cathode-electrolyte interphase (CEI) layer, endowing the cathode with the interfacial stability.  相似文献   

    

Dr. Sang Youn Chae  Noyoung Yoon  Dr. Oh shim Joo  Prof. Eun Duck Park 《Angewandte Chemie (International ed. in English)》2023,62(7):e202215227

The electrochemical activation of CuInS₂/MoS_x for photoelectrochemical (PEC) H₂ production was revealed for the first time through in operando Raman spectroscopy. During the activation process, the initial metallic MoS_x phase was transformed to semiconducting MoS_x, which facilitates charge carrier transfer between CuInS₂ and MoS_x. Ex situ X-ray photoelectron spectroscopy and Raman spectroscopy suggest the existence of MoO₃ after the activation process. However, apart from contradicting these results, in operando Raman spectroscopy revealed some of the intermediate steps of the activation process.  相似文献   

    

Muhammad Waseem Hafiz  Seong Oun Hwang 《Frontiers of Physics》2023,18(5):51304

The phenomenal progress of quantum information theory over the last decade has substantially broadened the potential to simulate the superposition of states for exponential speedup of quantum algorithms over their classical peers. Therefore, the conventional and modern cryptographic standards (encryption and authentication) are susceptible to Shor’s and Grover’s algorithms on quantum computers. The significant improvement in technology permits consummate levels of data protection by encoding classical data into small quantum states that can only be utilized once by leveraging the capabilities of quantum-assisted classical computations. Considering the frequent data breaches and increasingly stringent privacy legislation, we introduce a hybrid quantum-classical model to transform classical data into unclonable states, and we experimentally demonstrate perfect state transfer to exemplify the classical data. To alleviate implementation complexity, we propose an arbitrary quantum signature scheme that does not require the establishment of entangled states to authenticate users in order to transmit and receive arbitrated states to retrieve classical data. The consequences of the probabilistic model indicate that the quantum-assisted classical framework substantially enhances the performance and security of digital data, and paves the way toward real-world applications.  相似文献