Periodic Fermentor Yield and Enhanced Product Enrichment from Autonomous Oscillations

Four decades of work have clearly established the existence of autonomous oscillations in budding yeast culture across a range of operational parameters and in a few strains. Autonomous oscillations impact substrate conversion to biomass and products. Relatively little work has been done to quantify yield in this case. We have analyzed the yield of autonomously oscillating systems, grown under different conditions, and demonstrate that it too oscillates. Using experimental data and mathematical models of yeast growth and division, we demonstrate strategies to increase the efficient recovery of products. The analysis makes advantage of the population structure and synchrony of the system and our ability to target production within the cell cycle. While oscillatory phenomena in culture have generally been regarded with trepidation in the engineering art of bioprocess control, our results provide further evidence that autonomously oscillating systems can be a powerful tool, rather than an obstruction.     

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Eine Modifikation der Niclouxschen Methode zur Bestimmung von ?thylalkohol

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Is analytical chemistry a self-generating science or depending upon problems and ideas from other scientific areas?

Summary Some aspects on the situation of analytical chemistry as science are presented. Two examples can illustrate the influence of other sciences on the progress of analytical chemistry.     

Interaction of SWCNT and PPTA with sulfuric acid—Compatibilization of two materials in a common solvent

The results of a calorimetric study on the melting and crystallization behavior of concentrated sulfuric acid containing dispersed single wall carbon nanotubes (SWCNT), dissolved water, and dissolved poly‐p‐phenyleneterphthalate (PPTA) polymer are presented. The measured reduction of the heat of crystallization is caused by the build‐up of an associated layer of structured acid molecules around SWCNT and PPTA. The freezing point depression is related to the number of dissolved species such as ions. It is shown that this theory accurately describes the experimental data for dissolved water and sulfur trioxide, but not for large molecules such as SWCNT. A mechanism for the interaction between sulfuric acid and SWCNT is proposed, based on preferred adsorption of sulfur trioxide and dissociation of sulfuric acid, leading to an increased number of dissolved low‐molecular‐weight species. © 2008 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 46: 1914–1922, 2008