Development of a novel microfluidic immunoassay for the detection of Helicobacter pylori infection

The miniaturization of laboratory processes offers substantial advantages over traditional techniques in terms of cost, speed, and potential for multistage automation. To date, only a few studies have reported successful microfluidics-based immunoassays, most of which rely on fluorescence detection technologies. The goal of this study was to develop a poly(dimethylsiloxane) microfluidics-based immunoassay methodology and a versatile colorimetric quantification scheme for the detection of visual colour changes resulting from immune reactions in microchannels. The novel immunoassay technique was applied towards the detection of Helicobacter pylori infection using 20 human serum samples of known infection status, and results compared with conventional nitrocellulose membrane-based dot-ELISA. The microchannel immunoassay reliably detected H. pylori antigens in quantities on the order of 10 ng, which provides a sensitivity of detection comparable to conventional dot-blot assays. Sensitivity was 100%, specificity was 90%, positive predictive value 91%, and negative predictive value 100%, with an overall accuracy of 95%. The software developed generated results that were consistent with visual observations and by automatically taking into account background intensity changes, the software minimized subjectivity. Volumes of solutions used were 100-fold less compared with conventional immunoassays. Miniaturization of the ELISA using this technique provides a means for the accurate diagnosis of microbial infection while minimizing waste production.     

Temperatures and stresses in ablating hemispheres

Hypersonic wind-tunnel tests of thirteen 7 3/4-in.-diam Plexiglas and nylon hemispheres were designed and conducted at the Polytechnic Institute of Brooklyn utilizing a shrouded-model technique to simulate Mach 15 to 20 flight. The models were instrumented with thermocouples ans strain gages from which continous time histories of temperature and strain were recorded. In addition, ablation measurements were made after themodels were tested. The experimental results are compared with theoretical calculations of strain, temperature and ablation. Using a point-symmetric heat-balance method to determine the ablation and temperature histories, a thermal stress analysis is conducted in which variations in the modulus of elasticity with temperature are approximated by a concentric-layer technique. The effects of axisymmetric pressure distributions, as well as the effects of the boundary condition imposed by the model support, are considered in an axisymmetric alyer analysis. A comparison between theroy and experiment indicates good qualitative results and good-to-poor quantitative results. The details of the analyses, and the comparison between the theoretical predictions and experiment are discussed.     

Stability of oval cylindrical shells

Elastic buckling under aial compression of finite, oval cylindrical shells with clamped boundaries was investigated experimentally. The determination of the buckling strength was made on a series of oval shells made of Mylar A. The test results indicated that the discrepancy between theoretical and experimental initial buckling loads for the ovals is similar to that of the circular cylindrical shells. However, in contrast to the circular case, a collapse load significantly exceeding the initial buckling load is observed in the case of ovals with moderate-to-large eccentricity.     

Lipoide

Ohne Zusammenfassung     

Interactions of poly(amidoamine) dendrimers with Survanta lung surfactant: the importance of lipid domains

The interaction of generation 5 (G5) and 7 (G7) poly(amidoamine) (PAMAM) dendrimers with mica-supported Survanta bilayers is studied with atomic force microscopy (AFM). In these experiments, Survanta forms distinct gel and fluid domains with differing lipid composition. Nanoscale defects are induced by the PAMAM dendrimers. The positively charged dendrimers remove lipid from the fluid domains at a significantly greater rate than for the gel domains. Dendrimer accumulation on lipid edges and terraces preceding lipid removal has been directly imaged. Immediately following lipid removal, the mica surface is clean, indicating that lipid defects are not induced by dendrimers binding to the mica substrate and displacing the lipid.     

Mechanical Stimulation of Growth Plate Chondrocytes: Previous Approaches and Future Directions

Growth plate cartilage resides near the ends of long bones and is the primary driver of skeletal growth. During growth, both intrinsically and extrinsically generated mechanical stresses act on chondrocytes in the growth plate. Although the role of mechanical stresses in promoting tissue growth and homeostasis has been strongly demonstrated in articular cartilage of the major skeletal joints, effects of stresses on growth plate cartilage and bone growth are not well established. Here, we review the literature on mechanobiology in growth plate cartilage at macroscopic and microscopic scales, with particular emphasis on comparison of results obtained using different methodological approaches, as well as from whole animal and in vitro experiments. To answer these questions, macroscopic mechanical stimulators have been developed and applied to study mechanobiology of growth plate cartilage and chondrocytes. However, the previous approaches have tested a limited number of stress conditions, and the mechanobiology of a single chondrocyte has not been well studied due to limitations of the macroscopic mechanical stimulators. We explore how microfluidics devices can overcome these limitations and improve current understanding of growth plate chondrocyte mechanobiology. In particular, microfluidic devices can generate multiple stress conditions in a single platform and enable real-time monitoring of metabolism and cellular behavior using optical microscopy. Systematic characterization of the chondrocytes using microfluidics will enhance our understanding of how to use mechanical stresses to control the bone growth and the properties of tissue-engineered growth plate cartilage.

     

Kinetic collision detection between two simple polygons

We design a kinetic data structure for detecting collisions between two simple polygons in motion. In order to do so, we create a planar subdivision of the free space between the two polygons, called the external relative geodesic triangulation, which certifies their disjointness. We show how this subdivision can be maintained as a kinetic data structure when the polygons are moving, and analyze its performance in the kinetic setting.     

Dense Point Sets Have Sparse Delaunay Triangulations or “... But Not Too Nasty”

The spread of a finite set of points is the ratio between the longest and shortest pairwise distances. We prove that the Delaunay triangulation of any set of $n$ points in~$\Real^3$ with spread $\Delta$ has complexity $O(\Delta^3)$. This bound is tight in the worst case for all $\Delta = O(\sqrt{n})$. In particular, the Delaunay triangulation of any dense point set has linear complexity. We also generalize this upper bound to regular triangulations of $k$-ply systems of balls, unions of several dense point sets, and uniform samples of smooth surfaces. On the other hand, for any $n$ and $\Delta = O(n)$, we construct a regular triangulation of complexity $\Omega(n\Delta)$ whose $n$ vertices have spread $\Delta$.