Three novel oligosaccharides synthesized using Thermoanaerobacter brockii kojibiose phosphorylase

Background  

Recently synthesized novel oligosaccharides have been produced primarily by hydrolases and glycosyltransferases, while phosphorylases have also been subject of few studies. Indeed, phosphorylases are expected to give good results via their reversible reaction. The purpose of this study was to synthesis other novel oligosaccharides using kojibiose phosphorylase.     

Spectrofluorometric determination of hydrogen peroxide based on oxidative catalytic reactions of p-hydroxyphenyl derivatives with metal complexes of thiacalix[4]arenetetrasulfonate on a modified anion-exchanger.

The peroxidase-like catalytic activity of metal complexes of thiacalix[4]arenetetrasulfonate (TCAS[4]) on a modified anion-exchanger (Me(n+)-TCAS[4]A-500; Me(n+) = H2, Fe3+, Fe2+, Mn3+, Co3+, Co2+, Cu2+, Zn2+, Ni2+) for the oxidation of p-hydroxyphenyl derivatives to produce fluorescent substances in the presence of hydrogen peroxide has been investigated. Among the Me(n+)-TCAS[4]A-500 tested, Fe(3+)-TCAS[4]A-500 exhibited the highest level of catalytic activity for the oxidation of p-acetoamidophenol in a carbonate buffer solution of pH 10. The catalytic activity of Fe(3+)-TCAS[4]A-500 was then used for the spectrofluorometric determination of hydrogen peroxide. The calibration curve for the Fe(3+)-TCAS[4]A-500 method was linear over a range spanning from 0.1 to 5.0 microg of hydrogen peroxide in a 1.0 ml sample solution.     

Status of bubble detectors for high-energy heavy ions

Five types of bubble detectors (T-15, T-34, T-12, T-24 and T-14) of large volume and different sensitivities were developed in the China Institute of Atomic Energy for the purpose of heavy ion research. Calibrations were carried out with beams of high-energy protons, He, C, Si, Ar, Fe, Kr and Xe, at accelerators in the energy region 150–650 MeV/u. The bubble detectors were demonstrated to be a family of detectors for recording tracks of high-energy heavy ions with atomic numbers from Z=1 (proton) to all the numbers in the whole periodic table of elements. The threshold levels of the detectors differ from each other with values 0.05±0.01 (T-15), 1.62±0.05 (T-34), 1.68±0.03 (T-12), 2.24±0.06 (T-24) and 6.04±0.80 (T-14) MeVmg⁻¹ cm², respectively, which are about the same or even lower than the levels of plastic track detectors for recording heavy ions. The distinguishing features of bubble detectors are high sensitivity, active recording, real-time display, volume registration and background discriminating capacities. Bubble detectors are a new type of high sensitivity detectors and are very promising for detection of heavy ions, neutrons and exotic particles.     

Study of poly(methylphenylsilylenemethylene) by elastic thermalization lifetime analysis

A new elastic thermalization lifetime analysis (ETLA) is applied to lifetime spectra of polymethylphenylsilenemethylene (PMPSM) measured as a function of time after quick and slow cooling from room to liquid nitrogen temperature. The results show that in this material the efficiency of positronium yield is extremely high (90%). It is found that at low temperature the efficiency is changed by the positron source irradiation. A structural relaxation is observed when the sample is cooled slowly, which is indicated by a gradual decrease in the mean size of the free-volume holes.     

In situ size measurement of Si nanoparticles and formation dynamics after laser ablation

We have developed a technique to detect Si nanoparticles selectively and to measure size in situ. Applying the technique, we have investigated formation process of Si nanoparticles after pulsed laser ablation of Si targets in Ar gas. Time-resolved photoluminescence (PL) spectroscopy revealed that PL only from Si nanoparticles is observed below 2.4 eV while PL from Si nanoparticles as well as defects in SiO₂ is observed above 2.4 eV. Therefore, Si nanoparticles can be detected selectively by excitation light with a photon energy below 2.4 eV. It is found that the onset of the PL from Si nanoparticles is delayed by approximately 0.3 ms from that of the defects and smaller Si nanoparticles. A size can be estimated by a band gap, which is roughly equal to the lowest photon energy at which Si nanoparticles can be excited. Thus, we estimated the sizes of growing Si nanoparticles. PACS 61.46.+w; 78.66.w; 07.60.Yi     

π~0 Photoproduction on Deuteron for Photon Energies from 0.6 to 1.15 GeV

Differential and total cross sections of the γp→π~0 p and the γp→π~0 pn reaction were measured for photon energies between 640 and 1 150 MeV.The data have been compared with SAID and MAID model calculations.π~0 photoproduction on the deuteron shows a strong suppression of the 2nd resonance which is clearly seen in the γp→π~0 p reaction.     

Characterizations of Positive Linear Volterra Integro-differential Systems

We first give a criterion for positivity of the solution semigroup of linear Volterra integro-differential systems. Then, we offer some explicit conditions under which the solution of a positive linear Volterra system is exponentially stable or (robustly) lies in L²[0,+∞). The first and last author are supported by the Japan Society for Promotion of Science (JSPS) ID No. P 05049.     

Selective determination of cadmium(II) from divalent metal ions in environmental samples by capillary electrophoresis using in-capillary complexation with a lacunary Keggin-type [PW11O39]7- complex.

A novel capillary electrophoretic (CE) method, based on in-capillary complexation with [PW(11)O(39)](7-), was developed for the determination of cadmium(II) in natural water samples. When a sample solution is injected into a capillary containing 0.20 mM [PW(11)O(39)](7-) and 0.10 M malonate buffer (pH 3.0), the ternary Keggin-type complex, [P(Cd(II)W(11))O(39)](5-), which possesses high molar absorbtivities in the UV region, is formed in the capillary, and its migration toward the anode gives a well-defined migration peak in the electropherogram. An advantage of this method is that many divalent metal ions do not interfere. The proposed method was successfully applied to the determination of Cd(II) in environmental samples. The detection limits were 1 x 10(-7) and 5 x 10(-7) M for river-water and seawater samples, respectively (signal-to-noise ratio = 3).     

Autoparametric resonances in a structure/fluid interaction system carrying a cylindrical liquid tank

The nonlinear-coupled vibrations of an elastic structure and liquid sloshing in a cylindrical container are investigated. The behavior of the liquid surface is governed by a kind of the Mathieu equation because the structure is subjected to a vertical and sinusoidal excitation. Modal equations for liquid sloshing governing the coupled motions are derived when the natural frequency of the structure is equal to twice the natural frequency of an anti-symmetric mode of sloshing. The theoretical resonance curves are determined by using van der Pol's method. The influences of a liquid level and a detuning parameter on the theoretical resonance curves are investigated when only the excitation frequency is selected as a control parameter. The inclination of a frequency response curve depends on the liquid level. Furthermore, a small deviation of the tuning condition may cause amplitude- and phase-modulated motions and chaotic vibrations. This deviation also leads to separate the occurrence region of the coupled vibration into two regions of the excitation frequency. The theoretical resonance curves are quantitatively in agreement with the experimental data. Lastly, the amplitude- and phase-modulated motions and chaotic vibrations were observed in experiments.     

Asymptotic behavior of solutions of functional difference equations

For linear functional difference equations, we obtain some results on the asymptotic behavior of solutions, which correspond to a Perron-type theorem for linear ordinary difference equations. We also apply our results to Volterra difference equations with infinite delay.