Paclitaxel‐Loaded Stabilizer‐Free Poly(D,L‐lactide‐co‐glycolide) Nanoparticles Conjugated with Quantum Dots for Reversion of Anti‐Cancer Drug Resistance and Cancer Cellular Imaging

We prepared the PLGA‐loaded anti‐cancer drug and coated it with quantum dots to make it a dual‐function nanoparticles, and analyzed its potential use in cellular imaging and curing cancers. Two cancer cell lines, paclitaxel‐sensitive KB and paclitaxel‐resistant KB paclitaxel‐50 cervical carcinoma cells, were the relativistic models for analysis of the cytotoxicity of free paclitaxel and paclitaxel‐loaded PLGA conjugated with quantum‐dot nanoparticles. The paclitaxel‐loaded PLGA conjugated with quantum dots nanoparticles were significantly more cytotoxic than the free paclitaxel drug in paclitaxel‐resistant KB paclitaxel‐50 cells. This might have been because the cancer cells developed multi‐drug resistance (MDR), which hampered the action of free paclitaxel by pumping its molecules to extracellular areas. Addition of verapamil, a P‐glycoprotein inhibitor, reversed the MDR mechanism and significantly reduced KB paclitaxel‐50 cell viability. As a result, KB paclitaxel‐50 was highly associated with MDR on the cell membrane. The cytotoxicity results indicated that PLGA nanoparticles served as drug carriers and protected the drugs from MDR‐accelerated efflux. Combined quantum dots with PLGA nanoparticles allowed additional functionality for cellular imaging.     

Positive Charges in the Brace Region Facilitate the Membrane Disruption of MLKL-NTR in Necroptosis

Necroptosis is a type of programmed cell death executed through the plasma membrane disruption by mixed lineage kinase domain-like protein (MLKL). Previous studies have revealed that an N-terminal four-helix bundle domain (NBD) of MLKL is the executioner domain for the membrane permeabilization, which is auto-inhibited by the first brace helix (H6). After necroptosis initiation, this inhibitory brace helix detaches and the NBD can integrate into the membrane, and hence leads to necroptotic cell death. However, how the NBD is released and induces membrane rupture is poorly understood. Here, we reconstituted MLKL_2–154 into membrane mimetic bicelles and observed the structure disruption and membrane release of the first brace helix that is regulated by negatively charged phospholipids in a dose-dependent manner. Using molecular dynamics simulation we found that the brace region in an isolated, auto-inhibited MLKL_2–154 becomes intrinsically disordered in solution after 7 ns dynamic motion. Further investigations demonstrated that a cluster of arginines in the C-terminus of MLKL_2–154 is important for the molecular conformational switch. Functional mutagenesis showed that mutating these arginines to glutamates hindered the membrane disruption of full-length MLKL and thus inhibited the necroptotic cell death. These findings suggest that the brace helix also plays an active role in MLKL regulation, rather than an auto-inhibitory domain.     

表面绝缘铝平面丝阵Z箍缩实验研究

在"强光一号"装置(1.3 MA,100 ns)上进行了镀2μm聚酰亚胺绝缘膜平面铝丝阵与普通铝丝阵Z箍缩对比实验研究.实验结果表明,表面绝缘能够影响Z箍缩内爆动力学和辐射特性.通过简单理论分析,表面绝缘可能会增加初始阶段的能量沉积和参与内爆的质量;表面绝缘对于抑制平面丝阵多峰现象有明显作用,然而在所进行的实验中,并未观察到对X射线产额的改善作用.     

On linear and semidefinite programming relaxations for hypergraph matching

The hypergraph matching problem is to find a largest collection of disjoint hyperedges in a hypergraph. This is a well-studied problem in combinatorial optimization and graph theory with various applications. The best known approximation algorithms for this problem are all local search algorithms. In this paper we analyze different linear and semidefinite programming relaxations for the hypergraph matching problem, and study their connections to the local search method. Our main results are the following:

We consider the standard linear programming relaxation of the problem. We provide an algorithmic proof of a result of Füredi, Kahn and Seymour, showing that the integrality gap is exactly ${k-1+\frac{1}{k}}$ for k-uniform hypergraphs, and is exactly k ? 1 for k-partite hypergraphs. This yields an improved approximation algorithm for the weighted 3-dimensional matching problem. Our algorithm combines the use of the iterative rounding method and the fractional local ratio method, showing a new way to round linear programming solutions for packing problems.

We study the strengthening of the standard LP relaxation by local constraints. We show that, even after linear number of rounds of the Sherali-Adams lift-and-project procedure on the standard LP relaxation, there are k-uniform hypergraphs with integrality gap at least k ? 2. On the other hand, we prove that for every constant k, there is a strengthening of the standard LP relaxation by only a polynomial number of constraints, with integrality gap at most ${\frac{k+1}{2}}$ for k-uniform hypergraphs. The construction uses a result in extremal combinatorics.

We consider the standard semidefinite programming relaxation of the problem. We prove that the Lovász ${\vartheta}$ -function provides an SDP relaxation with integrality gap at most ${\frac{k+1}{2}}$ . The proof gives an indirect way (not by a rounding algorithm) to bound the ratio between any local optimal solution and any optimal SDP solution. This shows a new connection between local search and linear and semidefinite programming relaxations.

     

Dynamics of locally rotationally symmetric Bianchi type VIII cosmologies with anisotropic matter

This paper is a study of the effects of anisotropic matter sources on the qualitative evolution of spatially homogenous cosmologies of Bianchi type VIII. The analysis is based on a dynamical system approach and makes use of an anisotropic matter family developed by Calogero and Heinzle which generalises perfect fluids and provides a measure of deviation from isotropy. Thereby the role of perfect fluid solutions is put into a broader context. The results of this paper concern the past and future asymptotic dynamics of locally rotationally symmetric solutions of type VIII with anisotropic matter. It is shown that solutions whose matter source is sufficiently close to being isotropic exhibit the same qualitative dynamics as perfect fluid solutions. However a high degree of anisotropy of the matter model can cause dynamics to differ significantly from the vacuum and perfect fluid case.     

Xe对NO(X)微分截面和非弹性散射中的碰撞诱导旋转准直

本文对NO(X)-Xe碰撞系统在碰撞能量为519 cm^-1,测量了完全?-双峰分解的微分截面和碰撞引起的旋转准直力矩. 同时结合初始量子态选择,使用六极杆的非均质电场,借助量子态分辨的测量,利用(1+1'')共振增强的多光子电离和速度离子成像. 结果显示,微分截面以及偏振相关的微分截面均显示与从头算势能面上进行的量子力学散射计算[J. K?os etal. J. Chem. Phys. 137, 014312 (2012)]一致. 通过与准经典轨迹、硬壳势能的量子力学散射以及运动近端模型的比较,评估了势能对所测微分截面和碰撞引起的旋转准直力矩的影响.     

van der Waals状态方程与溶液理论

阐明了van der Waals状态方程与溶液理论间的密切关系。由van der Waals状态方程中的内压力和自由体积简捷地导得Scatchard-Hildebrand正规溶液理论、无热溶液理论和Flory-Huggins聚合物溶液理论。表明这些理论都是建立在van der Waals流体的基础上,为进一步改进这些理论提供了一条切实可行的思路。     

Kinetics and mechanism of electron-induced splitting of a cyclobutane pyrimidine dimer with or without an electron acceptor

DNA repair has received heightened attention in recent years as ozone depletion threatens to significantly increase DNA damage by UVB radiation[1—6]. The major lesions formed in DNA by this radiation are cis-syn cyclobutane pyrimidine dimers, which are created by the linkage of two neighboring pyrimidine bases in DNA via C5-C5 and C6-C6 atoms by [2+2] cycloaddition[2,5—8]. This potentially lethal or mutagenic damage can be repaired either by the removal of the damaged bases by excisio…     

VESTA软件在典型晶体结构教学中的应用

晶体结构的基本概念既多又抽象,没有较强的空间想象力,很难理解和辨析清楚。二维图缺乏空间感;实物模型只做出一个晶胞,其重复规律不直观。VESTA晶体结构软件能够根据需要构建多个三维晶胞,即超胞,可以很直观地显示出晶体结构的特点。本文通过几个教学实例,说明VESTA软件如何帮助学生深入理解晶体结构的基本概念,结构特点与性能的关系,以及拓展晶体结构的认知领域,提高学习兴趣与探索能力。     

Scalable Wet Deposition of Zeolite AEI with a High Degree of Preferred Crystal Orientation

Producing zeolite films with controlled preferred orientation on an industrial scale is a long‐standing challenge. Herein we report on a scalable approach to the direct wet deposition of zeolite thin films and membranes while maintaining a high degree of control over the preferred crystal orientation. As a proof of concept, thin films comprising aluminophosphate zeolite AEI were cast on silicon wafer or porous alumina substrates. Electrical properties and separation performance of the zeolite thin films/membranes were engineered through controlling degree of preferred crystal orientation.