Strategies for proteomics with incompletely characterized genomes: the proteome of Bos taurus serum

A reference map for Bos taurus serum was obtained using proteomic tools: 21 proteins, plus several serum albumin fragments, have been identified in 47 spots. One of the major acute-phase reactants, haptoglobin, was also detected in a pathological serum. A number of technical problems had to be solved. (i) Spot resolution in two-dimensional electrophoresis (2-DE) is not easily optimized, as several proteins have similar molecular mass; different polyacrylamide concentration gradients were used for the analysis of various size ranges. (ii) Identification of proteins through mass spectrometry (MS) procedures is also difficult as the genome of Bos taurus is incompletely characterized. The program FASTS proved particularly useful, since it allows simultaneous searching of several unordered sequence fragments, which may be individually too short to provide a statistically valid match using BLAST.     

Penalty methods for minimal surfaces with obstacles

Summary In this paper, we discuss an approach to the obstacle problem for minimal boundaries via penalty techniques. After investigating some classes of penalized problems, a general method is introduced, based on the minimization of a suitable functional containing an extra term related to the mean curvature of the given obstacle.     

The Chow group of the moduli space of marked cubic surfaces

Naruki gave an explicit construction of the moduli space of marked cubic surfaces, starting from a toric variety and proceeding with blow-ups and contractions. Using his result, we compute the Chow groups and the Chern classes of this moduli space. As an application we relate a recent result of Freitag on the Hilbert polynomial of a certain ring of modular forms to the Riemann–Roch theorem for the moduli space. Dedicated to the memory of our friend Fabio BardelliMathematics Subject Classification (2000) 14J15     

Assembling process of charged nonanuclear cationic lanthanide(III) clusters assisted by dichromium decacarbonyl hydride

The reaction of Ln(acac)(3).3H(2)O (Ln = Sm, Eu, Gd, Dy, Yb) with K[Cr(2)(CO)(10)(micro-H)] at different molar ratios and solvents leads to the formation of nonanuclear lanthanide hydroxo acetylacetonate complexes of general formula [Ln(9)(OH)(10)(acac)(16)][HCr(2)(CO)(10)]. The compounds are isomorphous, and the common cationic cluster core consists of a novel square antiprismatic arrangement of nine Ln atoms connected by micro(3), micro(4) hydroxo bridges and/or by acetylacetonate ligands as it results from the single-crystal X-ray analysis of the Sm derivative for which the most suitable crystals were obtained.     

Monitoring the redox-driven assembly/disassembly of a dicopper(I) helicate with an auxiliary fluorescent probe

The assembly/disassembly of a dicopper(I) helicate with a bis-bidentate imine-quinoline ligand is driven by the Cu(II)/Cu(I) redox change and is signaled by a fluorescent probe bearing a -COO(-) group (coumarine 343). The probe coordinates the Cu(II) center of the monomeric complex, which quenches its emission (fluorescence off), and is released upon reduction and formation of the Cu(I) helicate (fluorescence on).     

Polymer-supported haloarene chromium dicarbonyl isonitrile complexes: a study of their synthesis and reactivity

Different arene Cr(CO)(3) complexes were supported on a polystyrene isonitrile resin by photochemical-promoted replacement of a chromium carbonyl ligand by the NC group. The supported complexes proved to be stable and were successfully used for further transformations. In particular, the reactivity of dichlorobenzene complexes to different nucleophiles was investigated and found to be comparable with that of the parent Cr(CO)(3) complexes.     

Infinite Memory Expectations in a Dynamic Model with Hyperbolic Demand

In this study we will research the dynamics shown by a cobweb-type model with hyperbolic demand, sigmoidal supply and with backward-looking mechanism of expectation creation, whereby the new state of the system is obtained from all the previous states observed by weighted arithmetical mean with exponentially decreasing weights in the region. The study herewith presented aims at confirming the existence of a stabilising effect due to the presence of infinite memory since, with all the other conditions begin the same, a memory rate > exists at which market equilibrium is a sink. An unstable system, therefore, becomes stable in the presence of sufficiently resistant expectations with infinite historical memory, although this transition to stability is accompanied by the onset of chaos. The resulting effect, therefore is one of qualitative destabilisation, that is with reference to the qualitative dynamic performance produced, associated to a quantitative stabilisation, that is to say with reference to the decreasing width of the invariant sets within which relevant dynamics occur.     

The reaction of 2,3-diamino-butadienes with 1,2-diimmonium dibromides: A direct synthesis of 1,2,4-triamino benzenes

A new benzene ring synthesis is reported: 1,2-diimmonium dibromides react in mild conditions with 2,3-diamino-butadienes affording 1,2,4-triamino benzenes with yields of preparative interest.