Several benzofuroxans were obtained by photolysis of crystalline o-nitrophenylazides at ambient temperature. In this particular case, the solid matrix favored the elimination of nitrogen and exclusive formation of heterocyclic benzofuroxan. However, this reaction gives quantitative yields only with crystalline o-nitrophenylazides with a high melting point (above 50 °C). 相似文献
The reactivity of both the ester and amine functions present in β-amino esters was tested in order to obtain the synthesis of enantiopure αvβ3 and α5β1 integrin ligands. CaLB successfully catalyzed both the enantioselective transesterification and the N-acylation of racemic β-amino esters, allowing the isolation of intermediates for the preparation of Arg-Gly-Asp (RGD) mimetic compounds. In particular, a CaLB-catalyzed amidation reaction with unprotected p-aminobenzylamine reduced the number of synthetic steps, thus avoiding protection and deprotection of the intermediate compounds. Following this procedure, RGD mimetics were isolated with high yields and enantiomeric purities. 相似文献
An electronic measurement of high-voltages of several ten kV with accuracy as required for precision experiments is currently only feasible using highly sophisticated voltage dividers. Collinear laser spectroscopy can provide a direct and precise measurement of high-voltages using the Doppler shift of accelerated ions. Although proposed already in 1982, a measurement with relative accuracy better than 10?4 was not reported so far. To improve this accuracy, a dedicated new setup for high-voltage measurements will be installed at the Technische Universität Darmstadt. A two-chamber approach will be used to remove uncertainties due to the insufficiently known starting potential inside the ion source. Here we present a demonstration of the pump-and-probe technique performed in preparatory studies at the TRIGA-LASER experiment in Mainz. 相似文献
Platinum-rich CoPt films have been electrodeposited with the aim of preparing hard magnetic films on silicon-based substrates
without the need for subsequent annealing. Electrodeposition conditions have permitted the crystalline structure of the films
to be controlled. Pt percentages of up to 60–65 wt.% have been attained while maintaining the hexagonal Co phase, leading
to CoPt films with moderate coercivity and good corrosion resistance. However, when low deposition potentials were used, CoPt
films with higher Pt percentages were obtained, but in this case, the films exhibited an fcc structure, having lower coercivity
and less corrosion resistance. The presence of hypophosphite in the solution limited the platinum percentage in the deposited
CoPtP films, but an hexagonal close-packed (hcp) structure was always observed in this case. The incorporation of P into the
deposits led to increases in both the coercivity of the films and the corrosion resistance of the coatings, with respect to
pure CoPt. The highest coercivity was obtained for hcp CoPtP deposits with 40 wt.% of Pt. 相似文献
In developing tissues, proteins and signaling molecules present themselves in the form of concentration gradients, which determine the fate specification and behavior of the sensing cells. To mimic these conditions in vitro, we developed a microfluidic device designed to generate stable concentration gradients at low hydrodynamic shear and allowing long term culture of adhering cells. The gradient forms in a culture space between two parallel laminar flow streams of culture medium at two different concentrations of a given morphogen. The exact algorithm for defining the concentration gradients was established with the aid of mathematical modeling of flow and mass transport. Wnt3a regulation of β-catenin signaling was chosen as a case study. The highly conserved Wnt-activated β-catenin pathway plays major roles in embryonic development, stem cell proliferation and differentiation. Wnt3a stimulates the activity of β-catenin pathway, leading to translocation of β-catenin to the nucleus where it activates a series of target genes. We cultured A375 cells stably expressing a Wnt/β-catenin reporter driving the expression of Venus, pBARVS, inside the microfluidic device. The extent to which the β-catenin pathway was activated in response to a gradient of Wnt3a was assessed in real time using the BARVS reporter gene. On a single cell level, the β-catenin signaling was proportionate to the concentration gradient of Wnt3a; we thus propose that the modulation of Wnt3a gradients in real time can provide new insights into the dynamics of β-catenin pathway, under conditions that replicate some aspects of the actual cell-tissue milieu. Our device thus offers a highly controllable platform for exploring the effects of concentration gradients on cultured cells. 相似文献
When mass spectrometry is not combined to separation techniques, the evaluation of signal and noise in a complex mass spectrum
is not trivial. The tuning of the spectrometer based only on the increase of the signal of a selected number of m/z values does not ensure the achievement of the best experimental conditions: signal could improve and noise could increase
as well. The scope of this work is the development of a function separating signal and noise (for evaluating the S/N) from
complex mass spectra for potential use as target function for the automatic tuning of the instrument. Two different methods
were applied: the first is based on the separation of a pool of m/z values attributable to the signal from the m/z values due to the noise, while the second is based on the application of principal component analysis to separate the signal
(present in the significant components) from the noise (present in the residuals). The comparison of the two methods was carried
out by the evaluation of the stability of the signal and the target functions obtained, and the evaluation of the variation
of the target functions as a function of concentration. 相似文献
An anti trihydroxycarbenium ion is revealed to be the gas‐phase structure of protonated carbonic acid by IR multiple‐photon dissociation spectroscopy (see picture for calculated structure and comparison of experimental and computed spectra). Deprotonation yields anti‐H2CO3 with a nominal gas‐phase basicity of 724 kJ mol?1.