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111.
ULTRAVIOLET-INDUCED CELL DEATH IS INDEPENDENT OF DNA REPLICATION IN RAT KANGAROO CELLS 总被引:1,自引:0,他引:1
Rat kangaroo (Potorous tridactylus) cells have an efficient repair system for photoreactivation of lethal lesions induced by 254 nm UV. However, this ability is lost with increasing time after UV, being completely ineffective after 24 h. Critical events leading to UV-induced cell death must occur within this period of time. DNA synthesis was inhibited by the DNA polymerase inhibitor aphidicolin and the loss of the capability to photorepair lethal lesions was maintained as for replicating cells. Similar data were obtained in synchronized cells UV irradiated immediately before S phase. Under the same conditions, the ability to remove cyclobutane pyrimidine dimers by photoreactivation in these cells remained unchanged 24 h after irradiation. These data indicate that the critical events responsible for UV-induced cell death occur in the absence of DNA replication. 相似文献
112.
113.
Quantification of nitrogen compounds in diesel fuel samples by comprehensive two‐dimensional gas chromatography coupled with quadrupole mass spectrometry 下载免费PDF全文
Gabriela P. S. Maciel Maria E. Machado Michele E. da Cunha Eliane Lazzari Juliana M. da Silva Rosângela A. Jacques Laiza C. Krause Jamily A. S. Barros Elina B. Caramão 《Journal of separation science》2015,38(23):4071-4077
Although several methods for the analysis of nitrogen compounds in diesel fuel have been described in the literature, the demand for rapid, sensitive, and robust analyses has increased in recent years. In this study, a comprehensive two‐dimensional gas chromatographic method was developed for the identification and quantification of nitrogen compounds in diesel fuel samples. The quantification was performed using the standard addition method and the analysis was conducted using comprehensive two‐dimensional gas chromatography coupled with fast quadrupole mass spectrometry. This study is the first to report quantification of nitrogen compounds in diesel fuel samples using the standard addition method without fractionation. This type of analysis was previously performed using many laborious separation steps, which can lead to errors and losses. The proposed method shows good linearity for target nitrogen compounds evaluated (m‐toluidine, 4‐ethylaniline, indole, 7‐methylindole, 7‐ethylindole, carbazole, isoquinoline, 4‐methylquinoline, benzo[h]quinolone, and acridine) over a range from 0.05 to 2.0 mg/L, and limits of detection and quantification of <0.06 and 0.16 mg/L, respectively, for all nitrogen compounds studied. 相似文献
114.
115.
Marlène Bras Jean-Pierre Cloarec François Bessueille Eliane Souteyrand Jean-René Martin Jean-Paul Chauvet 《Journal of fluorescence》2000,10(3):247-247
A new methodology for the analysis of DNA polymorphisms has been developed using specific oligonucleotide strand arrays bound to a solid silicon support recovered by a thin layer of silica. Arrays of directly synthesized oligonucleotides covalently fixed on Si/SiO2 wafers have been designed at the macroscopic scale. Using suitable nucleotide-labeled units, the fluorescence emission technique has been used as an experimental control of the molecular network bound to the support and as a method for analyzing the hybridizing abilities of the corresponding oligonucleotide array. Fluorescein has allowed us to control the molecular density of the DNA strand resulting from a complete synthetic growing process. A specific protocol using both complementary and noncomplementary units labeled with two probes, Cy3 and Cy5, was used to distinguish clearly nucleotide units fixed on the array either as hybridized sequences or by the unavoidable adsorption process. The present performance of this fluorescence detection procedure will now be used with a scanning fluorescence device to perform the analysis at the microscopic scale. 相似文献
116.
Flávia C. de Souza Janaina C. da Rocha Eduardo Vieira Sergio A. S. Machado Eliane D'Elia 《Electroanalysis》2009,21(20):2277-2280
Many factors can affect the quality of diesel oil, in particular the degradation processes that are directly related to some organosulfur compounds. During the degradation process, these compounds are oxidized into their corresponding sulfonic acids, generating a strong acid content during the process. p‐Toluene sulfonic acid analysis was performed using the linear sweep voltammetry technique with a platinum ultramicroelectrode in aqueous solution containing 3 mol L?1 potassium chloride. An extraction step was introduced prior to the voltammetric detection in order to avoid the adsorption of organic molecules, which inhibit the electrochemical response. The extraction step promoted the transference of sulfonic acid from the diesel oil to an aqueous phase. The method was accurate and reproducible, with detection and quantification limits of 5 ppm and 15 ppm, respectively. Recovery of sulfonic acid was around 90%. 相似文献
117.
Eliane Teixeira Sousa Frederico de M. Rodrigues Cludio C. Martins Fabio Santos de Oliveira Pedro Afonso de P. Pereira Jailson B. de Andrade 《Microchemical Journal》2006,82(2):142-149
Peppers are used not only in cookery, but also in many other applications, like cosmetic, pharmaceutical and nourishing industry. The chemical composition of peppers is quite complex and several volatile and non-volatile substances contribute to their flavor, which is an important sensorial propriety. In this work a headspace/solid phase microextraction/gas chromatography coupled to mass spectrometry method was developed to evaluate the profiles of volatile compounds that contribute to the aroma of red, yellow and purple varieties of Capsicum chinense sp. peppers. The optimization of the extraction conditions was carried out using multivariate strategies such as factorial design and response surface methodology. The GC-MS analysis allowed the tentative identification of 34 compounds, with similarities higher than 85%, in accordance with the NIST mass spectral library. The data obtained by the analysis of volatile compounds, according to the proposed method, were treated with PCA chemometrics tool in order to group different varieties of C. chinense sp. peppers with similar VOC profiles. Amongst the most abundant VOCs, hexyl ester of pentanoic acid, dimethylcyclohexanols, humulene and esters of butanoic acid were found. Principal component analysis turned possible to visualize the grouping tendencies of the studied varieties of pepper, as well as the identification of the volatile compounds responsible for discriminating the three groups. Considering the fact that many species of peppers are used as human food, the significance of this work is further emphasized by its applicability to the study of food quality indicators, and as a tool for investigations on the composition of the pepper sources. 相似文献
118.
rico Marlon de Moraes Flores Fabiana E. Barcelos da Silva Eliane Pereira dos Santos Fvero Reisdorfer Paula Juliano Smanioto Barin Renato Zanella Valderi Luiz Dressler Celso Figueiredo Bittencourt 《Spectrochimica Acta Part B: Atomic Spectroscopy》2002,57(12):2095-2102
A procedure for the determination of arsenic by batch hydride generation atomic absorption spectrometry (HG AAS) in commercial samples of injectable drugs, containing high concentrations of Sb(V), is described. The procedure is based on the complexing effect for Sb of citric, oxalic and acetic acids as reaction media. Aqua regia was used for sample digestion prior to As determination by HG AAS. The following experimental conditions for the determination of total As, as As(V), were evaluated: the acid medium and its concentration, sodium tetrahydroborate concentration, purge time, and influence of the different oxidation states of As. The effect of the delay time after mixing of sample and acid solution was also studied. Optimized conditions were: 10% (m/v) citric acid, 1.5% (m/v) sodium tetrahydroborate solution and 30 s for purge time. A delay time of 1 h was required after the digested sample had been mixed with citric acid, before As determination could be carried out. No interference on As(III) and As(V) signals was observed in the presence of up to 1 mg Sb(V). The tolerance limits for Ni(II), Cu(II) and Pb(II) were 1 mg, 100 μg and 100 μg, respectively. Recovery tests for As(III) and As(V) resulted in values between 97 and 101%. Characteristic mass and detection limit (3σ), using the recommended conditions, were 0.52 and 0.8 ng, respectively, for total As. 相似文献
119.
João Carlos C.B. Soares de Mello Lidia Angulo Meza Juliana Quintanilha da Silveira Eliane Gonçalves Gomes 《European Journal of Operational Research》2013
It will be shown in this paper that the input oriented DEA BCC model can generate negative efficiencies that are usually hidden in the model. The impact of these negative efficiencies becomes obvious when using input oriented Cross Evaluation models. With the help of an example with one input and one output, the conditions for the possible occurrence of negative efficiencies will be shown. Furthermore, we will show that a small intuitive change in the BCC multipliers model, previously presented in other papers, corrects this situation. We show why this change is used and compared it with an alternative formulation, which avoid negative efficiencies, namely the Non-Decreasing Returns to Scale (NDRS) model. We also show that the formulation studied in this paper is less restrictive than the NDRS model. The study of this variation in the DEA BCC model will be complemented with the formulation of the dual envelope model. This model changes the original frontier. Using the concept of non-observed DMUs, those variations can be graphically analyzed. We have also carried out some algebraic studies concerning benchmarks, multipliers and returns to scale. 相似文献
120.
Sarra Zid Matthieu Zinet Eliane Espuche 《Journal of Polymer Science.Polymer Physics》2019,57(1):51-61
The optimization of polymer barrier properties is currently of crucial importance for a wide range of applications from packaging to building or even energy applications. To meet the requirements of these applications, polymer matrices are often combined with impermeable (nano) fillers. Different nanofiller natures, shapes, and contents have been experimentally used and a large range of barrier materials has been obtained. In the meantime, several numerical approaches have been developed to model gas diffusion properties of nanocomposite materials. However, these approaches often considered bidimensional systems. The aim of this work is to develop 3D Finite Element Model which would be used to predict gas barrier properties of nanocomposites for disk‐shaped nanofillers. The model thus obtained is valid in a wide range of fillers volume fraction values as well as aspect ratios, which makes it possible to go from diluted regimes to semidiluted or even concentrated ones. Furthermore, an analytical equation which describes gas diffusion through nanocomposites films has been built and validated with our finite element modeling model. © 2018 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2019 , 57, 51–61 相似文献