Application of microcolumnion chromatography using octadecylsilica immobilized with bovine serum albumin as stationary phase for the determination of inorganic anions

Microcolumn ion chromatography of inorganic anions has been studied using octadecylsilica immobilized with bovine serum albumin as a stationary phase. The analytes were monitored with a conductivity detector. The detection limits for chloride, nitrate and thiocyanate at S/N = 3 were 0.8, 1.0 and 3.0 μmol L^–1, respectively. The present system was applied to the determination of inorganic anions in tap water and serum samples. Received: 29 May 1996 / Accepted: 12 August 1996     

Methodology for analysis of displacement using digital holography

Digital holography is applied to make microdisplacement determination of a steel bar and an aluminum sheet. The displacement of aluminum sheet is made under immersion conditions in water and air. An experimental installation is presented for digital hologram registration with plane reference beam, using a CCD camera as detector. The “HOLODIG” computing system was developed for digital reconstruction of the image using the direct method, the intensity and phase image and interferograms were obtained. Taking into account the phase interferogram and making the unwrapping, the phase was determined for every point of the study surface. As examples of method application, the displacement of a square section steel bar, tested in air, and an aluminum sheet, tested in air and submerged in water, was determined. Experimental values of the displacement obtained agree with the applied ones. In the case of aluminum plate submerged in water, the magnitude of the measured displacement is proportional to water refraction index.