Photolyse des 3-Phenyl-2, 1-benzisoxazols und einiger seiner Derivate in Bromwasserstoffsäure

Photolysis of 3-phenyl-2, 1-benzisoxazole and some derivatives in hydrobromic acid The behaviour of 3-phenylanthranils (3-phenyl-2, 1-benzisoxazoles) towards photolysis in hydrobromic acid differs greatly from that in hydrochloric and sulfuric acid. Thus, reduction and substitution products are obtained. The formation of the reduction products involves hydrogen abstraction by a nitrenium ion species in the triplet state and that of the substitution products can be attributed to a subsequent S_E-bromination.     

The colored Hadwiger transversal theorem in ℝ<Superscript><Emphasis Type="Italic">d</Emphasis></Superscript>

Hadwiger’s transversal theorem gives necessary and suffcient conditions for a family of convex sets in the plane to have a line transversal. A higher dimensional version was obtained by Goodman, Pollack and Wenger, and recently a colorful version appeared due to Arocha, Bracho and Montejano. We show that it is possible to combine both results to obtain a colored version of Hadwiger's theorem in higher dimensions. The proofs differ from the previous ones and use a variant of the Borsuk-Ulam theorem. To be precise, we prove the following. Let F be a family of convex sets in ? ^d in bijection with a set P of points in ? ^d?1. Assume that there is a coloring of F with suffciently many colors such that any colorful Radon partition of points in P corresponds to a colorful Radon partition of sets in F. Then some monochromatic subfamily of F has a hyperplane transversal.     

Structure and conformational properties of N-pentafluorosulfur(sulfuroxide difluoride imide) SF5N=S(O)F2: vibrational analysis, gas electron diffraction, and quantum chemical calculations

The molecular structure and conformational properties of N-pentafluorosulfur(sulfuroxide difluoride imide), SF5N=S(O)F2, have been studied by vibrational spectroscopy (IR (gas) and Raman (liquid)), by gas electron diffraction (GED), and by quantum chemical calculations (MP2 and B3LYP with (6-31G(d) and 6-311+G(2df) basis sets). According to GED, the prevailing conformer possesses a syn structure (N-SF5 bond synperiplanar with respect to the bisector of the SF2 group). Splitting of the symmetric N=S=O stretching vibration in gas and liquid spectra demonstrates the presence of a second conformer (11(5)%) with anticlinal orientation of the N-SF5 bond according to quantum chemical calculations. The geometric structure, conformational properties, and vibrational frequencies are well reproduced by quantum chemical calculations.     

Molecules Present in Plant Essential Oils for Prevention and Treatment of Colorectal Cancer (CRC)

Essential oils (EOs) are a complex mixture of hydrophobic and volatile compounds synthesized from aromatic plants, commonly present in the human diet. In recent years, many in vitro studies have suggested possible anticancer properties of single EO compounds, on colorectal cancer (CRC) cells. However, the majority of these studies did not compare the effects of these compounds on normal and cancer colon cells. By using NCM-460, a normal human mucosal epithelial cell line, Caco-2, a human colon epithelial adenocarcinoma cell line, and SW-620, colon cancer cells derived from lymph node metastatic site, we identified cinnamaldehyde, derived from cinnamon EO and eugenol, derived from bud clove EO, as compounds with a specific anticancer action selectively targeting the transformed colonic cells. Both cinnamaldehyde (75 µM) and eugenol (800 µM), after 72 h of treatment, were capable to induce apoptosis, necrosis and a cell cycle slowdown in Caco-2 and in SW-620, but not in NCM-460 cells. If associated with a targeted delivery to the colon, these two compounds could prove effective in the prevention or treatment of CRC.     

Cold preservation of isolated hepatocytes in UW solution: experimental studies on the respiratory activity at 0 degrees C

To date, little attention has been paid to the role of the gas milieu in preservation solutions and its effect on cell viability. Dissolved O2 in the preservation media may be an important parameter to consider. In this study we polarographically measured the O2 concentration in air-equilibrated UW solution at 0 degrees C, as well as the respiratory activity of isolated hepatocytes cold-preserved in this solution up to 72 hours. To perform measurements at 0 degrees C, it was first necessary to characterize the sensor behavior at low temperatures. We verified that the sensor response is still linear at this temperature but the rate of response is significantly slower. The O2 solubility in UW-air solution at 0 degrees C was determined using a modified physical method and it was 410 microM O2, which, as expected, is lower than the solubility in water at the same temperature (453 microM O2). Isolated hepatocytes cold-stored in UW-air solution retained a measurable respiratory activity during a period of 72 hours. The O2 consumption rate was 0.48 +/- 0.13 nmol/O2/min/10(6) cells, which represents 1% of the control value at 36 degrees C (61.46 +/- 14.61 nmol/O2/min/10(6) cells). The respiratory activity and cell viability were well maintained during the preservation period. At present, preservation conditions need to be improved for cells to remain functionally active. Dissolved O2 may be required for energy re-synthesis but it also leads to an increment in reactive oxygen species. The O2 concentration in the preservation solution should be carefully controlled, reaching a compromise between cell requirement and toxicity.     

Enzymatic Chemoselective Aldehyde–Ketone Cross‐Couplings through the Polarity Reversal of Methylacetoin

The thiamine diphosphate (ThDP) dependent enzyme acetoin:dichlorophenolindophenol oxidoreductase (Ao:DCPIP OR) from Bacillus licheniformis was cloned and overexpressed in Escherichia coli. The recombinant enzyme shared close similarities with the acetylacetoin synthase (AAS) partially purified from Bacillus licheniformis suggesting that they could be the same enzyme. The product scope of the recombinant Ao:DCPIP OR was expanded to chiral tertiary α‐hydroxy ketones through the rare aldehyde–ketone cross‐carboligation reaction. Unprecedented is the use of methylacetoin as the acetyl anion donor in combination with a range of strongly to weakly activated ketones. In some cases, Ao:DCPIP OR produced the desired tertiary alcohols with stereochemistry opposite to that obtained with other ThDP‐dependent enzymes. The combination of methylacetoin as acyl anion synthon and novel ThDP‐dependent enzymes considerably expands the available range of C? C bond formations in asymmetric synthesis.     

Phase analysis of superconducting polycrystalline MgB(2)

Superconducting MgB(2) ceramics were prepared and yield superconducting transition temperatures of about 39 K. For covering the various length scales on which inhomogeneities appear in MgB(2), electron-probe micro-analysis (EPMA) and analytical transmission electron microscopy (TEM) were applied for a phase analysis. Particularly useful were the preliminary electron spectroscopic imaging (ESI) results in the TEM. It could be shown by EPMA that the microstructure consists of a Mg-B-O matrix and boron-rich secondary phases of composition close to MgB(12). It was unclear in which form oxygen was present in the superconducting matrix. By combining the acquisition of B-K and O-K edge jump ratio images and energy-dispersive X-ray spectroscopy in the TEM, we could prove that the matrix consists of superconducting MgB(2) and MgO. Most of the MgO precipitates and grains appear with diameters between 20 and 300 nm. The size distribution of MgO was inhomogeneous and oxygen-rich areas of dimensions >1 microm were also observed. Edge jump ratio images obtained by ESI were analysed for determining the signal values and effects of multiple inelastic scattering.     

Effective quantities and effective rules in two-dimensional ferromagnetic antidot lattices

In this paper the metamaterial properties of two-dimensional arrays of circular antidots (holes) embedded into a ferromagnetic medium of Permalloy are studied according to both micromagnetic and analytical calculations. The periodicity of the arrays and the diameters of the antidots are in the nanometric range. The collective mode dynamics is described by means of effective physical quantities for the scattering geometry with the external magnetic field applied perpendicularly to the Bloch wave vector in the antidot plane. As an example, the definition of an effective field, incorporating the demagnetizing effects due to the holes, permits to describe the dynamical properties of collective modes in terms of effective properties in the travelling regime. An effective wavelength and a small wave vector are introduced both for extended and localized magnonic modes. By means of these effective quantities it is shown that holes play the role of point defects affecting the spin dynamics in the microwave range. Relations between the effective wavelength and the Bloch wavelength and between the corresponding small wave vector and the Bloch wave vector are found. Some effective rules on the dynamic magnetization, based upon the effective wavelength and the corresponding small wave vector, are derived. An application that exploits the definition of the small wave vector is proposed and an experiment based upon the notion of effective wavelength and small wave vector is suggested.