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61.
An enzymatic solution to the problem of obtaining 13C/15N-labeled nucleotides that are deuterated uniquely at the H2' ' position within the ribose ring is presented. Selective deuteration occurs with an overall yield of >80%. The deuteron at the H2' ' position allows measurement of the scalar and residual dipolar couplings for the bond vectors attached to the C2' carbon of each ribose sugar. These data allow the accurate determination of sugar conformation. Interesting DNA double helices of 2-3 turns are now within the reach of solution NMR spectroscopy. As an example, these labeled nucleotides are incorporated uniquely at positions 6-14 in a 20-bp DNA sequence containing the adenovirus major late promoter. 相似文献
62.
Most common diseases appear to result from complex, poorly understood interactions between genetic and environmental factors. Relatively few factors have been unequivocally linked with disease risk or outcome. Evidence from various studies using different experimental approaches has been interpreted as showing that, apart from its harmful effects on the pathogenesis of the common skin cancers, ultraviolet radiation (UVR) may exert a beneficial effect on development of various internal cancers and other pathologies. This concept is supported by parallel studies showing that hypovitaminosis D is linked with increased risk of various diseases including insulin resistance and multiple sclerosis. These findings suggest that, first, host factors such as skin pigmentation that affect UVR-induced synthesis of vitamin D and, second, polymorphism in genes that mediate the effectiveness of vitamin D action are susceptibility candidates for a variety of diseases. Collectively, these data suggest the hypothesis that, via effects on vitamin D synthesis, UVR exposure has beneficial effects on susceptibility and outcome to a variety of complex diseases. We describe evidence from studies in various diseases, but mainly from prostate cancer patients, that supports this hypothesis, but we emphasize that, although supportive data are available, the concept is unproven. Indeed, other explanations are possible. However, given the potentially important public health implications of the hypothesis and the potential for the development of novel therapeutic modalities, we believe the concept is worthy of further investigation. 相似文献
63.
Uranium is determined via its 239U nuclide (74.0 keV, t = 23.5 min) in natural waters down to 0.03 ng U ml-1 after preconcentration with activated carbon and oxine; 30-min irradiation and counting times are used. No preconcentration is required for samples containing more than 4 ng U ml-1 with 10-min irradiation and counting times. Uranium in urine can be determined under a boron shield at the 5 ng ml-1 level after 30-min irradiation and counting. 相似文献
64.
Conductance measurements at 25°C have been carried out on Pr4NBr, Bu4NBr,n-Pe4NPr, Pr4NI, Bu4NI,n-Pe4NI, Bu4NClO4, Bu4NBF4, and Bu4PClO4 in isobutyl alcohol and on Bu4NBr and Bu4NClO4 in isopentyl alcohol. The main purpose of the measurements was to investigate the effect of increased branching in the alkyl portion of the alcohol on both ionic mobilities and ionic association. Comparison is made with the two isodielectric solvents butanone andn-butyl alcohol. Branching appears to produce little change in ionic mobilities and approximately a 10% increase in association. 相似文献
65.
Kihachiro Takahara Masahide Sasaki Arthur J. Tomisek Samuel Natelson 《Microchemical Journal》1974,19(3):319-329
A reagent is developed for increasing the sensitivity of the direct o-toluidine procedure for glucose so that the reaction may be carried out at temperatures as low as 37 °C.The sensitivity is achieved by eliminating all water from the system, except for a minute percentage introduced with the samples and by using a high boric acid concentration. Under these conditions protein does not precipitate and lipids remain dissolved. At 55 °C, interference from bilirubin at a concentration of 20 mg/100 ml is 3.5%.Blood may be collected with sodium fluoride since it does not interfere in the procedure. The rate of color development is slower for aqueous standards than it is for serum at the lower temperatures. This may be corrected by preparing the standards in a 6% albumin solution.Results obtained by applying the reagent with the continuous flow system of analysis (Technicon), and with the discrete sample analyzers, Beckman DSA, Lars Jungberg Autolab (Sweden), Robot Chemist, and RaBA (Japan) are reported.The reagent is useful as a spray reagent for sugar identification on thin-layer chromatography plates. Heating at different temperatures permits the differentiation between certain sugars with similar Rf values. 相似文献
66.
A non-destructive method for the quantification of eleven elements in bone samples is described. The analytical scheme is based on short (30 s) irradiation with thermal neutrons followed immediately (decay time 10 s) by counting fluorine-20 for 30 s and, after a total waiting time of 150 s, by 10-min γ-spectrometry counting, which give data for Ca, Cl, Mg, Mn, Na, and V. Use of a boron carbide shield for a second set of irradiations with epithermal neutrons permits the additional determination of bromine and strontium and calculation of the contribution of aluminium and phosphorus to the total activity of 28Al. 相似文献
67.
A heated injection system for a microprocessor-controlled GC has been developed for the (GC)2 analysis of the volatile phase of whole smoke of a cigarette. Effects of injection port temperature and the presence of a Cambridge filter pad are demonstrated. Chromatograms are shown for smoke samples with and without a Cambridge Filter with the sample valve oven at 25°, 165° and 205°C. The use of a flame ionization and a nitrogen-phosphorous detector is illustrated. 相似文献
68.
Hans Zimmer Adel Amer Douglas Ho R. Palmer-Sungail 《Journal of heterocyclic chemistry》1991,28(6):1501-1510
The reaction pathway of 4-aroyl-3-hydroxy-2(5H)-furanones 1 with diamines depends on the nature of the amine as well as on the applied reaction conditions. Thus, the reaction of 1a-d with 5,6-diamino-1,3-dimethyluracil 5 led to the formation of two isomeric Schiff bases 7a-d and 8a-d . Conversely type 1 compounds reacted with 4,5-diaminopyrimidine 9 or 2,3-diaminopyridine 10 to form the mono acid-base adducts 11a and 11b respectively. When type 1 compounds were reacted with aliphatic diamines 13a-d or p-phenylenediamine and p-xylenediamine, respectively also an immediate formation of acid-base adducts 15a-f was observed. The reaction of a number of O-methylated type 1 compounds with 1,2-ethylenediamine afforded the novel seven-membered ring compounds 18a-d in good yields. The analogous reaction of O-alkylated 1a with o-phenylenediamine 2 or 2,3-diaminonaphthalene gave the expected tricyclic ring systems 19 or 20 . 相似文献
69.
Samuel Natelson 《Microchemical Journal》1982,27(4):466-483
The existence of homoserine lactone in Pisum sativum seedlings is demonstrated. L-Homoserine lactone reacts with hydroxylamine, at neutral or alkaline pH, to form homoserine hydroxamic acid. Procedures are described for preparing L-homoserine lactone and L-homoserine hydroxamic acid. The hydroxamic acid yields a color with maximum absorbance at 492 nm with Fe3+ in 0.25 N HCl. This reaction permitted assay for total homoserine and homoserine lactone. Six-day old Pisum sativum seedlings, with cotyledons removed, were extracted with 90% ethanol. Evaporation of the ethanol and addition of Na2SO4 solution and toluene and centrifugation removed protein lipids and esters. After clarification with activated charcoal, homoserine lactone content was estimated by reaction with NH2OH and Fe3+ reagents. For total homoserine, protein precipitation was with 2 N HCl and toluene. Evaporation to dryness at 60 °C under vacuum converted all homoserine to the lactone. The values found for total homoserine (μmols/g, wet weight) and preformed lactone (%) with the various growth media used were as follows: nitrate 87.4 (14.7%), NH2OH 75.2 (6.3%), water 70.5 (7.9%), urea 56.4 (18.9%). Acetic anhydride added to homoserine hydroxamic acid forms acetohydroxamic acid, which yields a color with maximum absorbance at 505 nm with Fe3+. This color reaction is seven times as sensitive as the reaction of Fe3+ with homoserine hydroxamic acid itself. 相似文献
70.
Juliet D Risner Thomas P Nolan James Bentley Erol Girt Samuel D Harkness Robert Sinclair 《Microscopy and microanalysis》2007,13(2):70-79
For this analytical TEM study, nonmagnetic oxygen-rich boundaries were introduced into Co-Pt-alloy perpendicular recording media by cosputtering Co and Pt with TiO2. Increasing the TiO2 content resulted in changes to the microstructure and elemental distribution within grains and boundaries in these films. EFTEM imaging was used to generate composition maps spanning many tens of grains, thereby giving an overall depiction of the changes in elemental distribution occurring with increasing TiO2 content. Comparing EFTEM with spectrum-imaging maps created by high-resolution STEM with EDXS and EELS enabled both corroboration of EFTEM results and quantification of the chemical composition within individual grain boundary areas. The difficulty of interpreting data from EDXS for these extremely thin films is discussed. Increasing the TiO2 content of the media was found to create more uniformly wide Ti- and O-rich grain boundaries as well as Ti- and O-rich regions within grains. 相似文献