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Mixed dissociation constants of four drug acids, i.e. silychristin, silybinin, silydianin and mycophenolate at various ionic strengths I of range 0.01 and 0.30 and at temperatures of 25 and 37 °C were determined using the SQUAD(84) regression analysis program applied to pH-spectrophotometric titration data. The proposed strategy of an efficient experimentation in a protonation constants determination, followed by a computational strategy for the chemical model with a protonation constants determination, is presented on the protonation equilibria of silychristin. The thermodynamic dissociation constant pKaT was estimated by non-linear regression of {pKa, I} data at 25 and 37 °C: for silychristin pKa,1T=6.52(16) and 6.62(1), pKa,2T=7.22(13) and 7.41(5), pKa,3T=8.96(9) and 8.94(9), pKa,4T=10.17(7) and 10.03(8), pKa,5T=11.89(4) and 11.63(7); for silybin pKa,1T=7.00(4) and 6.86(5), pKa,2T=8.77(11) and 8.77(3), pKa,3T=9.57(8) and 9.62(1), pKa,4T=11.66(3) and 11.38(1); for silydianin pKa,1T=6.64(7) and 7.10(6), pKa,2T=7.78(5) and 8.93(1), pKa,3T=9.66(9) and 10.06(11), pKa,4T=10.71(7) and 10.77(7), pKa,5T=12.26(5) and 12.14(5); for mycophenolate pKaT=8.32(1) and 8.14(1). Goodness-of-fit tests for various regression diagnostics enabled the reliability of parameter estimates to be found. 相似文献
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A flow-injection system for detection of alkaline phosphatase (ALP) activity in human serum samples has been developed. As a specific and inexpensive ALP substrate for this kinetic assay monofluorophosphate (MFP) was applied. For detection of fluoride ions, generated in the course of the biocatalytic hydrolysis of MFP, conventional fluoride ion-selective electrode based on LaF3-crystalline membrane was applied. After optimization the system allows analysis of human serum with high selectivity and relatively short time of analysis (5–6 samples h−1). Volume of serum required for analysis is 0.05 mL. The system is useful for determination of the enzyme activity in human serum samples at physiological and pathological levels as well as for detection of isoenzymatic forms of ALP. 相似文献
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Dominika Sersenov Zdenko Machala Vanda Repisk Helena Gbelcov 《Molecules (Basel, Switzerland)》2021,26(14)
Plasma medicine is a new field focusing on biomedical and clinical applications of cold gas plasmas, including their anticancer effects. Cold plasmas can be applied directly or indirectly as plasma-activated liquids (PAL). The effects of plasma-activated cell growth medium (PAM) and plasma-activated phosphate buffered saline (PAPBS) were tested, using a plasma pen generating streamer corona discharge in ambient air, on different cancer cell lines (melanoma A375, glioblastoma LN229 and pancreatic cancer MiaPaCa-2) and normal cells (human dermal fibroblasts HDFa). The viability reduction and apoptosis induction were detected in all cancer cells after incubation in PAL. In melanoma cells we focused on detailed insights to the apoptotic pathways. The anticancer effects depend on the plasma treatment time or PAL concentration. The first 30 min of incubation in PAL were enough to start processes leading to cell death. In fibroblasts, no apoptosis induction was observed, and only PAPBS, activated for a longer time, slightly decreased their viability. Effects of PAM and PAPBS on cancer cells showed selectivity compared to normal fibroblasts, depending on correctly chosen activation time and PAL concentration, which is very promising for potential clinical applications. This selectivity effect of PAL is conceivably induced by plasma-generated hydrogen peroxide. 相似文献
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&#x;ukasz Sobczak Dominika Ko&#x;odziej Krzysztof Gory&#x;ski 《Molecules (Basel, Switzerland)》2021,26(15)
Octadecyl (C18) groups are arguably the most popular ligands used for preparation of solid phase microextraction (SPME) devices. However, conventional C18-bonded silica particles are not fully compatible with the nearly 100% aqueous composition of typical biological samples (e.g., plasma, saliva, or urine). This study presents the first evaluation of thin-film SPME devices coated with special water-compatible C18-bonded particles. Device performance was assessed by extracting a mixture of 30 model compounds that exhibited various chemical structures and properties, such as hydrophobicity. Additionally, nine unique compositions of desorption solvents were tested. Thin-film SPME devices coated with C18-bonded silica particles with polar end-capping groups (10 µm) were compared with conventional trimethylsilane end-capped C18-bonded silica particles of various sizes (5, 10, and 45 µm) and characteristics. Polar end-capped particles provided the best extraction efficacy and were characterized by the strongest correlations between the efficacy of the extraction process and the hydrophobicity of the analytes. The results suggest that the original features of octadecyl ligands are best preserved in aqueous conditions by polar end-capped particles, unlike with conventional trimethylsilane end-capped particles that are currently used to prepare SPME devices. The benefits associated with this improved type of coating encourage further implementation of microextractraction as greener alternative to the traditional sample preparation methods. 相似文献
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Tomas Jasenovec Dominika Radosinska Marta Kollarova Peter Balis Kristina Ferenczyova Barbora Kalocayova Monika Bartekova Lubomira Tothova Jana Radosinska 《Molecules (Basel, Switzerland)》2021,26(16)
Diabetes mellitus is characterized by tissue oxidative damage and impaired microcirculation, as well as worsened erythrocyte properties. Measurements of erythrocyte deformability together with determination of nitric oxide (NO) production and osmotic resistance were used for the characterization of erythrocyte functionality in lean (control) and obese Zucker diabetic fatty (ZDF) rats of two age categories. Obese ZDF rats correspond to prediabetic (younger) and diabetic (older) animals. As antioxidants were suggested to protect erythrocytes, we also investigated the potential effect of quercetin (20 mg/kg/day for 6 weeks). Erythrocyte deformability was determined by the filtration method and NO production using DAF-2DA fluorescence. For erythrocyte osmotic resistance, we used hemolytic assay. Erythrocyte deformability and NO production deteriorated during aging—both were lower in older ZDF rats than in younger ones. Three-way ANOVA indicates improved erythrocyte deformability after quercetin treatment in older obese ZDF rats only, as it was not modified or deteriorated in both (lean and obese) younger and older lean animals. NO production by erythrocytes increased post treatment in all experimental groups. Our study indicates the potential benefit of quercetin treatment on erythrocyte properties in condition of diabetes mellitus. In addition, our results suggest potential age-dependency of quercetin effects in diabetes that deserve additional research. 相似文献
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Amanda Grylewicz Kacper Szyma&#x;ski Dominika Darowna Sylwia Mozia 《Molecules (Basel, Switzerland)》2021,26(9)
Ultrafiltration polyethersulfone (PES) membranes were prepared by wet phase inversion. Commercial halloysite nanotubes (HNTs) in the quantities of 0.5 wt% vs. PES (15 wt%) were introduced into the casting solution containing the polymer and different solvents: N,N-dimethylformamide (DMF), N,N-dimethylacetamide (DMA), or 1-methyl-2-pyrrolidinone (NMP). The type of solvent influenced the membranes’ morphology and topography, as well as permeability, separation characteristics, and antifouling and antibacterial properties. The membranes prepared using DMA exhibited the loosest cross-section structure with the thinnest skin and the roughest surface, while the densest and smoothest were the DMF-based membranes. The advanced contact angles were visibly lower in the case of the membranes prepared using DMF compared to the other solvents. The highest water permeability was observed for the DMA-based membranes, however, the most significant effect of the modification with HNTs was found for the NMP-based series. Regardless of the solvent, the introduction of HNTs resulted in an improvement of the separation properties of membranes. A noticeable enhancement of antifouling performance upon application of HNTs was found only in the case of DMF-based membranes. The study of the antibacterial properties showed that the increase in surface roughness had a positive effect on the inhibition of E. coli growth. 相似文献
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Dominika Kozon Jolanta Mierzejewska Tomasz Kobiela Agnieszka Grochowska Ksenia Dudnyk Agnieszka G&#x;ogowska Anna Sobiepanek Aleksandra Ku
mi&#x;ska Tomasz Ciach Ewa Augustynowicz‐Kope&#x; Dominik Ja&#x;czewski 《Macromolecular bioscience》2019,19(12)
Polycations, mimicking activity of antibacterial peptides, belong to an important class of molecules investigated as a support or as an alternative to antibiotics. In this work, studies of modified linear amphiphilic statistical polymethyloxazoline (PMOX) and polyethyleneimine copolymers (PMOX_PEI) series are presented. Variation of PEI content in the structure results in controllable changes of polymeric aggregates zeta potential. The structure with the highest positive charge shows the best antimicrobial activity, well visible in tests against model Gram‐positive and Gram‐negative bacteria, fungi, and mycobacterium strains. The polymer toxicity is evaluated with MTT and hemolysis assay as a reference. Quartz crystal microbalance (QCM‐D) is used to investigate interaction between polycations and a model lipid membrane. Polymer activity correlates well with molecular structure, showing that amphiphilic component is altering polymer behavior in contact with the lipid bilayer. 相似文献
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Dr. Dominika Wawrzyńczyk Dr. Urszula Bazylińska Dr. Łukasz Lamch Dr. Julita Kulbacka Anna Szewczyk Dr. Artur Bednarkiewicz Prof. Kazimiera A. Wilk Prof. Marek Samoć 《ChemSusChem》2019,12(3):706-719
Multilayer nanocarriers loaded with optically activated payloads are gaining increasing attention due to their anticipated crucial role for providing new mechanisms of energy transfers in the health-oriented applications, as well as for energy storage and environmental protection. The combination of careful selection of optical components for efficient Förster resonance energy transfer, and surface engineering of the nanocarriers, allowed us to synthesize and characterize novel theranostic nanosystems for diagnosis and therapy of deep-seated tumors. The cargo, constrained within the oil core of the nanocapsules, composed of NaYF4:Tm+3, Yb+3 up-converting nanoparticles together with a second-generation porphyrin-based photosensitizing agent—Verteporfin, assured requisite diagnostic and therapeutic functions under near-IR laser excitation. The outer polyaminoacid shell of the nanocapsules was functionalized with a ligand—poly(l -glutamic acid) functionalized by PEG-ylated folic acid—to ensure both a “stealth” effect and active targeting towards human breast cancer cells. The preparation criteria of all nanocarrier building blocks meet the requirements for sustainable and green chemistry practices. The multifunctionality of the proposed nanocarriers is a consequence of both the surface-functionalized organic exterior part, which was accessible for selective accumulation in cancer cells, and the hydrophobic optically active interior, which shows phototoxicity upon irradiation within the first biological window. 相似文献
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