锌制剂“维宝康”治疗儿童缺锌综合征的临床研究

用维宝康治疗小儿缺锌综合征９８例,结果表明,服用维宝康一个疗程后,患者临床症状明显改善发锌值升高,总有效率为９６％,未见毒副作用,是较好的补锌制剂。     

Converse theorems for multidimensional Kantorovich operators

L _p [0, l]. . . - .

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Supported by National Science Foundation, Zhejiang Provincial Science Foundation of China, and Alexander von Humboldt Foundation of Germany.     

Novel reference gene, High-mobility-group protein I/Y, used in qualitative and real-time quantitative polymerase chain reaction detection of transgenic rapeseed cultivars

With the development of transgenic crops, regulations to label the genetically modified organisms (GMOs) and their derived products have been issued in many countries. Polymerase chain reaction (PCR) methods are thought to be reliable and useful techniques for qualitative and quantitative detection of GMOs. These methods are generally needed to amplify the transgene and compare the amplified results with that of a corresponding reference gene to get the reliable results. Specific primers were developed for the rapeseed (Brassica napus), high-mobility-group protein I/Y(HMG-I/Y) single-copy gene and PCR cycling conditions suitable for the use of this sequence as an endogenous reference gene in both qualitative and quantitative PCR assays. Both methods were assayed with 15 different rapeseed varieties, and identical amplified products were obtained with all of them. No amplification was observed when templates were the DNA samples from the other species of Brassica genus or other species, such as broccoli, stem mustard, cauliflower, Chinese cabbage, cabbage, sprouts, Arabidopsis thaliana, carrot, tobacco, soybean, mung bean, tomato, pepper, eggplant, plum, wheat, maize, barley, rice, lupine, and sunflower. This system was specific for rapeseed. Limits of detection and quantitation in qualitative and quantitative PCR systems were about 13 pg DNA (about 10 haploid genomes) and about 1.3 pg DNA (about 1 haploid genome), respectively. To further test the feasibility of this HMG-I/Y gene as an endogenous reference gene, samples containing transgenic rapeseed GT73 with the inserted glyphosate oxidoreductase (GOX) gene were quantitated. These demonstrated that the endogenous PCR detection systems were applicable to the qualitative and quantitative detection of transgenic rapeseed.     

等色染料离子对萃取光度法研究：Ⅰ.溴酚蓝·亚甲蓝体系

研究了溴酚蓝（ＢＰＢ）·亚甲蓝（ＭＢ）等色染料离子对萃取光度测定银、汞和铜的方法。首先将被测金属离子形成［Ｍｅ（ｐｈｅｎ）３］ＢＰＢ的二氯乙烷萃取液，用ＫＣＮ解析并反萃入水相，而后加入和溴酚蓝等色的亚甲蓝溶液继续萃取。由于等色染料离子对和Ｍｅ（ＣＮ）ｎＢＰＢ三元络合物同时进入二氯乙烷中而提高了灵敏度、测定Ａｇ、Ｈｇ和Ｃｕ的摩尔吸光系数均在１０５Ｌ·ｍｏｌ（－１）·ｃｍ（－１）以上。