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491.
Measurement of subcellular texture by optical Gabor-like filtering with a digital micromirror device
We demonstrate an optical Fourier processing method to quantify object texture arising from subcellular feature orientation within unstained living cells. Using a digital micromirror device as a Fourier spatial filter, we measured cellular responses to two-dimensional optical Gabor-like filters optimized to sense orientation of nonspherical particles, such as mitochondria, with a width around 0.45 microm. Our method showed significantly rounder structures within apoptosis-defective cells lacking the proapoptotic mitochondrial effectors Bax and Bak, when compared with Bax/Bak expressing cells functional for apoptosis, consistent with reported differences in mitochondrial shape in these cells. By decoupling spatial frequency resolution from image resolution, this method enables rapid analysis of nonspherical submicrometer scatterers in an undersampled large field of view and yields spatially localized morphometric parameters that improve the quantitative assessment of biological function. 相似文献
492.
Dimitris Alexandropoulos Hercules Simos Spiros Mikroulis Dimitris Syvridis 《Optics Communications》2008,281(3):421-425
Polarization properties of active semiconductor micro-ring structures are studied theoretically emphasizing on the prospects for equalising the structure response for the two polarizations. The evaluation is performed with the help of a scheme for polarization insensitive operation of active MR that, unlike previous approaches, addresses both the waveguide and the heterostructure polarization properties. A self-consistent rate equation model is used to assess the spectral response of the polarization insensitive structures. Based on these findings the inherent spectral limitations for polarization insensitive performance imposed by the highly resonant nature of micro-ring are discussed and compared to that of conventional structures. 相似文献
493.
Dimitris I. Ioannou Prof. Dr. Luca Capaldo Jiri Sanramat Prof. Dr. Joost N. H. Reek Prof. Dr. Timothy Noël 《Angewandte Chemie (International ed. in English)》2023,62(52):e202315881
Electrophotocatalytic transformations are garnering attention in organic synthesis, particularly for accessing reactive intermediates under mild conditions. Moving these methodologies to continuous-flow systems, or flow ElectroPhotoCatalysis (f-EPC), showcases potential for scalable processes due to enhanced irradiation, increased electrode surface, and improved mixing of the reaction mixture. Traditional methods sequentially link photochemical and electrochemical reactions, using flow reactors connected in series, yet struggle to accommodate reactive transient species. In this study, we introduce a new flow reactor concept for electrophotocatalysis (EPC) that simultaneously utilizes photons and electrons. The reactor is designed with a transparent electrode and employs cost-effective materials. We used this technology to develop an efficient process for electrophotocatalytic heteroarylation of C(sp3)−H bonds. Importantly, the same setup can also facilitate purely electrochemical and photochemical transformations. This reactor represents a significant advancement in electrophotocatalysis, providing a framework for its application in flow for complex synthetic transformations. 相似文献
494.
Bertsimas Dimitris Hertog Dick den Pauphilet Jean Zhen Jianzhe 《Mathematical Programming》2023,200(2):877-918
Mathematical Programming - Robust convex constraints are difficult to handle, since finding the worst-case scenario is equivalent to maximizing a convex function. In this paper, we propose a new... 相似文献