Synthesis of dual‐functional poly(6‐azidohexylmethacrylate) brushes by a RAFT agent carrying carboxylic acid end groups

Novel types of dual‐functional surface‐attached polymer brushes were developed by interface‐mediated reversible addition‐fragmentation chain transfer (RAFT) polymerization of 6‐azidohexylmethacrylate using the surface‐immobilized RAFT agent and the free initiator. The interface‐mediated RAFT polymerization produced silicon substrate coated with dual‐functional (azido groups from monomer and carboxylic acid groups from RAFT agent) poly(6‐azidohexylmethacrylate) [poly (AHMA)] with a grafting density as high as 0.59 chains/nm². Dual‐functional polymer brushes can represent an attractive chemical platform to deliberately introduce other molecular units at specific sites. The azido groups of the poly(AHMA) brushes can be modified with alkyl groups via click reaction, known for their DNA hybridization, while the carboxylic acid end groups can be reacted with amine groups via amide reaction, known for their antifouling properties. © 2015 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2015 , 53, 1696–1706     

高分子担载席夫碱及其Cr（Ⅲ）配合物：合成、表征、抗菌和抗真菌性能评价

用胺甲基树脂与5-氟-2-羟基苯甲醛,5-氟-3-氯-2-羟基苯甲醛,5-氟-3-甲基-2-羟基苯甲醛反应合成了3种新颖的高分子担载席夫碱和它们Cr（Ⅲ）配合物,并用元素分析、磁化率、IR、1HNMR、UV-Vis、TG/DTA对其进行了表征。就一些革兰氏阳性菌（芽孢杆菌属腊状芽孢杆菌（Bacillus cereus sp）,单核细胞增生李斯特菌4b（Listeria monocytogenes 4b）,藤黄微球菌（Micrococcus luteus）,金黄色葡萄球菌（Staphylococcus aureus）表皮葡萄球菌（Staphylococcus epidermis））和革兰氏阴性菌（布鲁氏菌（Brucella abortus）,大肠杆菌（Escherichia coli）,恶臭假单胞菌SP（Pseudomonas putidasp.）,痢疾志贺氏菌10（Shigella dysenteria type 10）,伤寒杆菌H（Salmonella typhi H）和酵母菌（念珠菌）（yeast（Candida albicans））用井扩散法对高分子担载席夫碱和它们Cr（Ⅲ）配合物的生物学活性进行了筛选。     

The copolymerization of allyl glycidyl ether with acrylonitrile initiated by gamma-rays

Copolymers of allyl glycidyl ether (AGE) with acrylonitrile (AN) have been prepared by bulk polymerization of their monomers with gamma rays. Copolymers thus obtained were characterized by Fourier transform infrared (FTIR), and ultraviolet (UV) spectroscopic techniques. The composition of the copolymers is determined indirectly by FTIR, UV, and directly by elemental analysis. The results obtained by different methods are compared. The reactivity ratios of monomer pairs (AGE + AN) which copolymerized heterogeneously were calculated by using different methods of determination. Among the three experimental methods used for the analysis of compositions and two theoretical methods of computations, the elemental analysis technique and the application of nonlinear least-squares method gave the most reliable reactivity ratios. These are found to be 1.86 and 0.21 for acrylonitrile and allyl glycidyl ether, respectively. © 1996 John Wiley & Sons, Inc.     

A New Series of Indeno[1,2-c]pyrazoles as EGFR TK Inhibitors for NSCLC Therapy

Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related death throughout the world. Due to the shortcomings of traditional chemotherapy, targeted therapies have come into prominence for the management of NSCLC. In particular, epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) therapy has emerged as a first-line therapy for NSCLC patients with EGFR-activating mutations. In this context, new indenopyrazoles, which were prepared by an efficient microwave-assisted method, were subjected to in silico and in vitro assays to evaluate their potency as EGFR TK-targeted anti-NSCLC agents. Compound 4 was the most promising antitumor agent towards A549 human lung adenocarcinoma cells, with an IC₅₀ value of 6.13 µM compared to erlotinib (IC₅₀ = 19.67 µM). Based on its low cytotoxicity to peripheral blood mononuclear cells (PBMCs), it can be concluded that compound 4 exerts selective antitumor action. This compound also inhibited EGFR TK with an IC₅₀ value of 17.58 µM compared to erlotinib (IC₅₀ = 0.04 µM) and induced apoptosis (56.30%). Taking into account in silico and in vitro data, compound 4 stands out as a potential EGFR TKI for the treatment of NSCLC.     

Electrochemical sensor based on a sepiolite clay nanoparticle-based electrochemical sensor for ascorbic acid detection in real-life samples

Ionics - A new electrode composed of sepiolite clay (SC) carbon paste (CP) was developed and used for the adsorptive stripping differential pulse voltammetric quantification of ascorbic acid (AA)....     

Modified gold surfaces by poly(amidoamine) dendrimers and fructose dehydrogenase for mediated fructose sensing

An electrochemical biosensor for detection of fructose in food samples was developed by immobilization of fructose dehydrogenase (FDH) on cysteamine and poly(amidoamine) dendrimers (PAMAM)-modified gold electrode surface. Electrochemical analysis was carried out by using hexacyanoferrate (HCF) as a mediator and the response time was 35 s at +300 mV vs. Ag/AgCl. Moreover, some parameters such as pH, enzyme loading and type of PAMAM (Generations 2, 3 and 4) were investigated. Then, the FDH biosensor was calibrated for fructose in the concentration range of 0.25–5.0 mM. To evaluate its utility, the FDH biosensor was applied for fructose analysis in real samples. Finally, obtained data were compared with those measured with HPLC as a reference method.     

Phase transition in Cu2+-doped RbH2 PO4 as observed by EPR

The electron paramagnetic resonance (EPR) spectra of Cu²⁺-doped RbH₂ PO₄ at elevated temperatures indicate a phase transition at 358 K. The EPR-silent state at this temperature is attributed to a so-called polymeric phase transition. After the transition when the temperature is lowered to 293 K, the EPR signal does not appear; therefore, the transition is irreversible. This result seems to be in agreement with the other observations. The EPR spectra for the sample indicate the presence of two sites for Cu²⁺, and the values of EPR parameters are in accord with the literature on Cu²⁺-doped single crystals. Any other phase transitions could not to be observed at low temperatures down to 113 K.     

Poly(p‐phenylene) with Poly(ethylene glycol) Chains and Amino Groups as a Functional Platform for Controlled Drug Release and Radiotherapy

Conventional cancer treatments such as chemotherapy, radiotherapy, or combination of these two result in side effects, which lower the quality of life of the patients. To overcome problems with these methods, altering the drug properties by conjugating them to carrier polymers has emerged. Such polymeric carriers also hold the potential to make tumor cells more sensitive to radiation therapy. Herein, poly(p‐phenylene) (PPP) polymer with poly(ethylene glycol) (PEG) chains and primary amino groups (PPP‐NH₂‐g‐PEG) is synthesized and conjugated with anticancer drug Doxorubicin (DOX). pH dependent drug release experiments are performed at pH 5.3 and pH 7.4, respectively. Cell viability studies on human cervix adenocarcinoma cells show that lower doses of DOX inhibit cell proliferation when conjugated with nontoxic doses of PPP‐NH₂‐g‐PEG polymer. Additionally, PPP‐NH₂‐g‐PEG/Cys/DOX bioconjugate significantly increases radiosensitive properties of DOX. It is possible to use lower doses of DOX when conjugated to PPP‐NH₂‐g‐PEG in combination with radiotherapy.