Glycopeptide and Oligosaccharide Libraries

Despite the burgeoning interest in the various biological functions and consequent therapeutic potential of the vast number of oligosaccharides found in nature on glycoproteins and cell surfaces, the development of combinatorial carbohydrate chemistry has not progressed as rapidly as expected. The reason for this imbalance is rooted in the difficulty of oligosaccharide assembly and analysis that renders synthesis a rather cumbersome endeavor. Parallel approaches that generate series of analogous compounds rather than real libraries have therefore typically been used. Since generally low affinity is obtained for interactions between carbohydrate receptors and modified oligosaccharides designed as mimetics of natural carbohydrate ligands, glycopeptides have been explored as alternative mimics. Glycopeptides have been proven in many cases to be superior ligands with higher affinity for a receptor than the natural carbohydrate ligand. High-affinity glycopeptide ligands have been found for several types of receptors including the E-, P-, and L-selectins, toxins, glycohydrolases, bacterial adhesins, and the mannose-6-phosphate receptor. Furthermore, the assembly of glycopeptides is considerably more facile than that of oligosaccharides and the process can be adapted to combinatorial synthesis with either glycosylated amino acid building blocks or by direct glycosylation of peptide templates. The application of the split and combine approach using ladder synthesis has allowed the generation of very large numbers of compounds which could be analyzed and screened for binding of receptors on solid phase. This powerful technique can be used generally for the identification and analysis of the complex interaction between the carbohydrates and their receptors.     

Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis

A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single‐chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer‐controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3 mg L^?1 of O₂ in the bulk solution, decreasing to 0 mg L^?1 in the cathodic biofilm. The MFC generated power in the range of 0–0.08 mW, associated to the oxygen content.     

ZnS(111)表面掺杂Mn的电子结构和磁性的第一性原理研究

应用基于密度泛函理论的第一性原理,研究Mn原子掺杂在ZnS(111)表面的电子结构和磁性.对于单原子的掺杂组态,替位表面第一层的Zn原子时体系形成能最低,说明该层是最稳定的掺杂位置.体系总磁矩取决于Mn原子的局域环境.而对于双掺杂组态,当Mn与Mn之间呈短程铁磁耦合作用时体系最稳定.这可由Mn原子和近邻S原子的p-d杂化作用解释.此时,体系的居里温度估算值为469 K,明显高于室温,具有理论指导意义.Mn原子和受主半导体之间的相互作用是自旋极化产生的主要原因.计算结果表明,该掺杂材料可以很好的用来制作稀磁半导体,具有良好的应用前景.     

Sensitive method for the determination of rocilinostat in small volume mouse plasma by LC‐MS/MS and its application to a pharmacokinetic study in mice

A highly sensitive, specific and rapid LC‐ESI‐MS/MS method has been developed and validated for the quantification of rocilinostat in small volume mouse plasma (20 μL) using vorinostat as an internal standard (IS) as per regulatory guidelines. Sample preparation was accomplished through a protein precipitation procedure with acetonitrile. Chromatography was achieved on Prodigy ODS‐2 column using a binary gradient using mobile phase A (0.2% formic acid in water) and B (acetonitrile) at a flow rate of 0.38 mL/min. The total chromatographic run time was 4.1 min and the elution of rocilinostat and IS occurred at ~3.2 and 2.9 min, respectively. A linear response function was established in the concentration range of 0.28–1193 ng/mL in mouse plasma. The intra‐ and inter‐day accuracy and precisions were in the ranges of 3.12–8.93 and 6.41–11.6%, respectively. This novel method has been applied to a pharmacokinetic study in mice. Copyright © 2016 John Wiley & Sons, Ltd.     

Prion protein self-peptides modulate prion interactions and conversion

Background  

Molecular mechanisms underlying prion agent replication, converting host-encoded cellular prion protein (PrP^C) into the scrapie associated isoform (PrP^Sc), are poorly understood. Selective self-interaction between PrP molecules forms a basis underlying the observed differences of the PrP^C into PrP^Sc conversion process (agent replication). The importance of previously peptide-scanning mapped ovine PrP self-interaction domains on this conversion was investigated by studying the ability of six of these ovine PrP based peptides to modulate two processes; PrP self-interaction and conversion.     

对二维分离流涡黏性系数非线性分布的新认识

以弱非线性涡黏性模型为出发点,对Delery分离流动实验结果进行分析并获得了非平衡态分离区涡黏性系数与形状因子J之间的非线性关系. 该非线性关系显示在分离起始阶段,涡黏性系数较平衡态先减小,后增大;再附阶段,涡黏性系数较平衡态数值逐渐增大,并在再附点位置接近最大,而后又逐渐减小,恢复到平衡态水平. 总结涡黏性系数的这种非线性发展数学关系式,并将它应用于BL模型,在不添加微分方程的情况下发展出一种适用于分离流动的改进代数湍流模型. 对低速平板流动,跨声速,超声速以及高超声速分离流动的计算结果表明,该改进湍流模型可以较准确地模拟各类复杂分离流动,计算精度明显优于传统代数模型以及一些两方程模型,而计算工作量仍与BL模型相当. 这表明所提出的涡黏性系数非线性发展规律是正确的,且应用在二维分离流动中具有一定的普适性.      

MQX-RTOS底层设备驱动构件可复用性研究

针对MQX实时操作系统下如何有效复用已经成熟的底层设备驱动构件,提高操作系统下底层设备驱动软件的开发速度、效率和质量等问题;在详细分析和研究MQX实时操作系统IO设备驱动三层管理体系构架基础上,将底层设备驱动和操作系统剥离,提出了MQX四层设备驱动管理体系框架;通过设计底层设备驱动接口构件,成功地复用了已有的底层设备驱动构件,避免底层驱动软件重复开发,有效缩短嵌入式软件开发的周期,并通过实例证明了该体系框架的可行性。 