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排序方式: 共有1819条查询结果,搜索用时 15 毫秒
901.
Kaj Backfolk Rob Holmes Petri Ihalainen Petri Sirvi Nick Triantafillopoulos Jouko Peltonen 《Polymer Testing》2007,26(8):1031-1040
The glass transition temperature (Tg) for two latices with different styrene/butadiene compositions was determined by the thermal SPM probe resonance frequency method. The results were compared with the Tg values obtained by differential scanning calorimetry (DSC), dynamical mechanical analysis (DMA), process rheometer (PR) and thermo-mechanical analyzer (TMA) measurements. The Tg values detected by the thermal SPM method agreed well with the Tg values obtained by DSC and calculated by the Fox–Flory equation. DMA, on the other hand, showed a significantly higher Tg value for both latices than those obtained from theoretical calculations, the thermal SPM method and DSC. The Tg obtained from the PR curve was slightly higher for the latex with a low styrene content, whereas good agreement was obtained with the thermal SPM data for the latex with a high styrene content. The glass transition temperature determined by TMA agreed fairly well with the thermal SPM data for the latex with the low styrene content, whilst the value of Tg for the second latex was much less than those obtained by the other methods. The thermal SPM method detects changes in thermal behavior (thermal diffusivity, heat capacity) during heating of the latex films rather than changes in the mechanical properties. Information about the sample history could be seen in the thermal SPM curves, which was further associated with the degree of latex film formation, especially when the roughness of the films was taken into consideration. 相似文献
902.
M. C. Freitas A. M. G. Pacheco 《Journal of Radioanalytical and Nuclear Chemistry》2007,271(1):185-189
Mass and elemental concentrations in PM10 and PM2.5 — airborne particulate matter with an aerodynamic diameter below 10 μm and 2.5 μm, respectively — have been evaluated in
mainland Portugal since 1993. The data herein refer to areas generally impacted by emissions from electrical power plants,
either coal- or oil-fired, which are mostly large, state-of-the-art facilities. Background areas have been sampled as well,
and results from one of them are given for comparison. Through the country, lead levels are well below the European enacted
values, particularly since unleaded fuels became mandatory in the late 1990s. Nickel appears especially concentrated in PM2.5 and, apart from extreme cases (rural sites, coal-fired plants), its levels are not significantly different from place to
place. Highest values for arsenic and mercury have been found in the vicinity of the coal-fired plants. Cadmium data, if only
available for metropolitan Lisbon, seems to confirm the trend for anthropogenic elements appearing mainly in fine particulates. 相似文献
903.
The HIV-1 Dimerization Initiation Site (DIS) is an intriguing, yet underutilized, viral RNA target for potential antiretroviral therapy. To study the recognition features of this target and to provide a quantitative, rapid, and real-time tool for the discovery of new binders, a fluorescence-based assay has been constructed. It relies on strategic incorporation of 2-aminopurine, an isosteric fluorescent adenosine analogue, into short hairpin RNA constructs. These oligomers self-associate to form a kissing loop that thermally rearranges into a more stable extended duplex, thereby mimicking the association and structural features of the native RNA sequence. We demonstrate the ability of two fluorescent DIS constructs, DIS272(2AP) and DIS273(2AP), to report the binding of known DIS binders via changes in their emission intensity. Binding of aminoglycosides such as paromomycin to DIS272(2AP) results in significant fluorescence enhancement, while ligand binding to DIS273(2AP) results in fluorescence quenching. These observations are rationalized by comparison to the sequence-analogous bacterial A-site, where the relative emission of the fluorescent probe is dependent on the placement of the flexible purine residues inside or outside the helical domain. Analysis of binding isotherms generated using DIS272(2AP) yields submicromolar EC50 values for paromomycin (0.5 +/- 0.2 microM) and neomycin B (0.6 +/- 0.2 microM). Other neomycin-family aminoglycosides are less potent binders with neamine, the core pharmacophore, displaying the lowest affinity of 21 +/- 1 microM. Screening of additional aminoglycosides and their derivatives led to the discovery of new, previously unreported, aminoglycoside binders of the HIV DIS RNA, among them butirosin A (5.5 +/- 0.6 microM) and apramycin (7.6 +/- 1.0 microM). A conformationally constrained neomycin B analogue displays a rather high affinity to the DIS (1.9 +/- 0.2 microM). Among a series of nucleobase aminoglycoside conjugates, only the uracil derivatives display a measurable affinity using this assay with EC50 values in the 2 microM range. In addition, similarity between the solution behavior of HIV-1 DIS and the bacterial decoding A-site has been observed, particularly with respect to the intra- and extra-helical residence of the conformationally flexible A residues within the bulge. Taken together, the observations reported here shed light on the solution behavior of this important RNA target and are likely to facilitate the design of new DIS selective ligands as potential antiretroviral agents. 相似文献
904.
Human very-low-density lipoprotein (VLDL) particles were immobilised on the inner wall of electrochromatographic fused-silica capillaries, and the applicability of these capillary columns in oxidation studies was investigated. Capillaries coated with radiolabelled VLDL particles showed a coating efficiency of 97%, and allowed estimation of the amount of VLDL present in a capillary. Radioactivity measurements and atomic force microscopy with tapping mode confirmed the presence of VLDL particles as a monolayer. The pI determined for the VLDL was 4.7-4.8 varying with the human source. The effects of VLDL concentration, coating time and pH on the coating stability were clarified, and the stability was examined in terms of the repeatability of EOF and retention factors of selected steroids. The repeatability of run-to-run and the coating-to-coating reproducibility ranged from 2.6 to 4.9% and 3.2 to 6.6%, respectively. The lifetime of a coating was at least 7 days or 84 consecutive runs. The in situ copper-mediated VLDL oxidation carried out in the capillary with optimised VLDL coating showed that, during the oxidation of VLDL particles, the negative charges of the particles are increased, leading to enhanced EOF mobilities. Several oxidation parameters, including copper sulfate concentration, amount of EDTA needed to stop the reaction, pH and the oxidation procedure, were examined. Effect of the oxidation process on the stability of the coating in one capillary, and in five different capillaries ranged between 0.4-4.1% and 0.8-6.6%, respectively. The in situ oxidation of VLDL particles was compared with that of low-density lipoproteins. 相似文献
905.
Two unusual flavonoids, 3,5,4',5'-tetrahydroxy-7-methoxy-6-[1-(p-hydroxy-phenyl)ethyl]flavanone (1) and 3,5,7,4',5'-pentahydroxy-6-[1-(p-hydroxy-phenyl)ethyl] flavanone (2), were isolated from the kino of Eucalyptus citriodora. Structural elucidation of the new compounds were established on the basis of spectral data, particularly by the use of 1D NMR and several 2D shift-correlated NMR pulse sequences ((1)H, (1)H-COSY, HMQC, HMBC). 相似文献
906.
Colnaghi Simionato AV da Silva DS Lambais MR Carrilho E 《Journal of mass spectrometry : JMS》2007,42(10):1375-1381
Xylella fastidiosa (X.f.) is a plant pathogen with high levels of genomic similarity to Xanthomonas campestris pv. campestris (X.c.c.). It has been shown that X. fastidiosa synthesizes a putative diffusible signal factor (X.f.-DSF) that activates regulation of pathogenicity factor (rpf) genes in a X.c.c. reporter system, which might be involved in the regulation of pathogenesis associated genes as in X.c.c., as well as in quorum-sensing. The nature of the X.f.-DSF is not known, whereas the X.c.c.-DSF has been identified as cis-11-methyl-2-dodecenoic acid. In this work, the chemical nature of a putative X.f.-DSF molecule, able to restore endoglucanase activity in a X.c.c. rpfF mutant, was investigated as if it was a fatty acid derivative. Bioassays with X.c.c. reporter bacterium and X.f. culture extracts, based on endoglucanase restoration activity, were also carried out in order to confirm the DSFs molecules similarities. For this reason, a gas chromatography-mass spectrometry method was developed with standard fatty acids methyl esters mixtures. The retention time, as well as the fragmentation patterns, of each standard was used to identify the DSF molecule synthesized by X.f. in the culture medium. Typical ester fragmentation patterns (the derivatized analyte) were observed, such as: McLafferty rearrangement and migration of the Hdelta followed by 1,4-hydrogen shift and cleavage of the bond Cbeta--Cgamma, confirming the nature of this molecule. This confirmation was corroborated by the common peaks in both spectra. Besides, the observed retention time reinforces our conclusion since it corresponds to a methyl ester with 15 carbons. Since the X.f.-DSF molecule was tentatively identified as 12-methyl-tetradecanoic acid (by mass spectra library comparison), this standard compound was also analyzed, strongly suggesting that this is the identification of such a molecule. To our knowledge, this is the first time a DSF produced by X.f. has been characterized. 相似文献
907.
pH-biased isoelectric trapping was used to separate proteins from egg white at the preparative level (80 mg), into discrete protein fractions based on isoelectric point. The problems of isoelectric precipitation that are common for the separation of complex protein mixtures under isoelectric conditions were mitigated by using single-component isoelectric buffers within the sample separation compartments. This combined with the mild process conditions of the Gradiflow unit that was modified for binary isoelectric trapping separations, ensured that biological activity was maintained. This was verified by measurement of the trypsin protease inhibitory activity of the extract and separated fractions. Furthermore, the high resolving power of this system under preparative conditions was demonstrated by separation of three protein isoforms using isoelectric membranes with differences of 0.025 pH units from each other. 相似文献
908.
Cássia Alves de Freitas Ticiane Sanches Valera Adriana Martinelli Catelli de Souza Nicole Raymonde Demarquette 《Macromolecular Symposia》2007,247(1):260-270
In this work, the evolution of the morphology of polypropylene/polystyrene/poly(methyl metacrylate) (PP/PS/PMMA) blends to which graft copolymers polypropylene-graft-polystyrene (PP-g-PS) of 2 compositions (55/45 and 70/30), polypropylene-graft-poly(methyl metacrylate) (PP-g-PMMA), or styrene-block-(ethylene- co-butadiene)-block-styrene (SEBS) was added has been studied. The ternary blends morphologies were predicted using phenomenological models that predict the morphology of ternary blends as a function of the interfacial tension between the blend components (spreading coefficient and free energy minimization). All blends studied presented a core-shell morphology with PS as shell and PMMA as core. The addition of PP-g-PS or SEBS resulted in a reduction of the size of the PS shell phase and, the addition of PP-g-PMMA did not seem to have any effect on the diameter of PMMA. The difference observed between the different morphologies relied on the number of droplets of core within the shell. All the phenomenological models predictions corroborated the experimental results, except when PP-g-PMMA was added to the blend. 相似文献
909.
910.
Soares KV Masini JC Torresi RM Carmona-Ribeiro AM Petri DF 《Langmuir : the ACS journal of surfaces and colloids》2005,21(18):8515-8519
The synthesis of hybrid particles was carried out by emulsion polymerization of styrene in complexes formed by carboxymethyl cellulose (CMC), a polyanion, and a cationic surfactant, cetyltrimethylammonium bromide (CTAB). CMC chains with variable molecular weights and degrees of substitution were tested. The polymerization condition chosen was that corresponding to CMC chains fully saturated with CTAB and to the onset of pure surfactant micelle formation, namely, at the critical aggregation concentration. The hybrid particles were characterized by zeta potential and light scattering measurements. The period of colloidal stability in the ionic strength of 2.0 mol L(-)(1) NaCl was observed visually. Upon increasing the CMC chain length, the particle characteristics remained practically unchanged, but the colloid stability was increased. The increase in the CMC degree of substitution led to particles with more negative zeta potential values. The adsorption of copper ions (Cu(2+)) on the surface of hybrid particles could be described by the Langmuir model, as determined by potentiometric measurements. The increase in the mean zeta potential values and X-ray absorption near-edge spectra evidenced the immobilization of Cu(2+) ions on the hybrid particles. 相似文献