全文获取类型
收费全文 | 253篇 |
免费 | 2篇 |
国内免费 | 1篇 |
专业分类
化学 | 197篇 |
力学 | 2篇 |
数学 | 30篇 |
物理学 | 27篇 |
出版年
2022年 | 6篇 |
2021年 | 6篇 |
2020年 | 4篇 |
2019年 | 4篇 |
2018年 | 2篇 |
2017年 | 2篇 |
2016年 | 1篇 |
2015年 | 1篇 |
2014年 | 5篇 |
2013年 | 13篇 |
2012年 | 16篇 |
2011年 | 19篇 |
2010年 | 8篇 |
2009年 | 10篇 |
2008年 | 12篇 |
2007年 | 20篇 |
2006年 | 17篇 |
2005年 | 15篇 |
2004年 | 11篇 |
2003年 | 15篇 |
2002年 | 9篇 |
2001年 | 5篇 |
2000年 | 1篇 |
1999年 | 3篇 |
1997年 | 3篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1990年 | 5篇 |
1989年 | 5篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1979年 | 1篇 |
1978年 | 4篇 |
1977年 | 4篇 |
1975年 | 1篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1972年 | 1篇 |
1968年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有256条查询结果,搜索用时 15 毫秒
61.
C Chirivì G Fontana D Monti G Ottolina S Riva B Danieli 《Chemistry (Weinheim an der Bergstrasse, Germany)》2012,18(33):10355-10361
Laccase-catalysed oxidation of ergot alkaloids in the absence of chemical mediators allowed the unexpected isolation of the mono-hydroxylated derivatives of compounds 2-7. Structure determination by NMR techniques clearly indicated that hydroxylation took place at the C-4 benzylic position. Quite notably, the proposed protocol allowed, for the first time, functionalisation at the C-4 position of the ergoline skeleton. Depending on the absence or on the presence of a C-10 α-methoxy substituent, hydroxylation was either stereoselective (furnishing C-4α OH derivatives) or gave rise to a C-4α/C-4β OH mixture in a 2:1 ratio, respectively. 相似文献
62.
Li Y Gridley CL Jaeger J Sweasy JB Schlick T 《Journal of the American Chemical Society》2012,134(24):9999-10010
Mutations in DNA polymerase β (pol β) have been associated with approximately 30% of human tumors. The E295K mutation of pol β has been linked to gastric carcinoma via interference with base excision repair. To interpret the different behavior of E295K as compared to wild-type pol β in atomic and energetic detail, we resolve a binary crystal complex of E295K at 2.5 ? and apply transition path sampling (TPS) to delineate the closing pathway of the E295K pol β mutant. Conformational changes are important components in the enzymatic pathway that lead to and ready the enzyme for the chemical reaction. Our analyses show that the closing pathway of E295K mutant differs from the wild-type pol β in terms of the individual transition states along the pathway, associated energies, and the active site conformation in the final closed form of the mutant. In particular, the closed state of E295K has a more distorted active site than the active site in the wild-type pol β. In addition, the total energy barrier in the conformational closing pathway is 65 ± 11 kJ/mol, much higher than that estimated for both correct (e.g., G:C) and incorrect (e.g., G:A) wild-type pol β systems (42 ± 8 and 45 ± 7 kJ/mol, respectively). In particular, the rotation of Arg258 is the rate-limiting step in the conformational pathway of E295K due to unfavorable electrostatic and steric interactions. The distorted active site in the closed relative to open state and the high energy barrier in the conformational pathway may explain in part why the E295K mutant is observed to be inactive. Interestingly, however, following the closing of the thumb but prior to the rotation of Arg258, the E295K mutant complex has a similar energy level as compared to the wild-type pol β. This suggests that the E295K mutant may associate with DNA with similar affinity, but it may be hampered in continuing the process of chemistry. Supporting experimental data come from the observation that the catalytic activity of wild-type pol β is hampered when E295K is present: this may arise from the competition between E295K and wild-type enzyme for the DNA. These combined results suggest that the low insertion efficiency of E295K mutant as compared to wild-type pol β may be related to a closed form distorted by unfavorable electrostatic and steric interactions between Arg258 and other key residues. The active site is thus less competent for proceeding to the chemical reaction, which may also involve a higher reaction barrier than the wild-type or may not be possible in this mutant. Our analysis also suggests further experiments for other mutants to test the above hypothesis and dissect the roles of steric and electrostatic factors on enzyme behavior. 相似文献
63.
Japaridze NJ Okuneva VG Qsovreli MG Surmava AG Lordkipanidze TG Kiladze MT Zhvania MG 《Micron (Oxford, England : 1993)》2012,43(9):948-953
Porosomes are the universal secretory machinery in cells, where membrane-bound secretory vesicles transiently dock and fuse to release intravesicular contents to the outside of the cell during cell secretion. Studies using atomic force microscopy, electron microscopy, electron density and 3D contour mapping, provided rich nanoscale information on the structure and assembly of proteins within the neuronal porosome complex in normal brain. However it remains uncertain whether pathological conditions that alter process of neurotransmission, provoke alterations in the porosome structure also. To determine if porosomes are altered in disease states, the current study was undertaken for first time using high resolution electron microscope. One of pathologies that produce subtle alteration at the presynaptic terminals has been demonstrated to be hypokinetic stress. The central nucleus of amygdale is the brain region, where such alterations are mostly expressed. We have examined the width and depth of the neuronal porosome complex and their alterations provoked by chronic hypokinetic stress in above mentioned limbic region. Specifically, we have demonstrated that despite alterations in the presynaptic terminals and synaptic transmission provoked by this pathological condition in this region, the final step/structure in neurosecretion--the porosome--remains unaffected: the morphometric analysis of the depth and diameter of this cup-shaped structure at the presynaptic membrane point out to the heterogeneity of porosome dimensions, but with unchanged fluctuation in norm and pathology. 相似文献
64.
We define relative Goursat categories and prove relative versions of the equivalent conditions defining regular Goursat categories. These include 3-permutability of equivalence relations, preservation of equivalence relations under direct images, a condition on so-called Goursat pushouts, and the denormalised 3×3 Lemma. This extends recent work by Gran and Rodelo on a new characterisation of Goursat categories to a relative context. 相似文献
65.
Dr. Nivesh Kumar Nadim Eghbarieh Dr. Tamar Stein Dr. Alexander I. Shames Prof. Dr. Ahmad Masarwa 《Chemistry (Weinheim an der Bergstrasse, Germany)》2020,26(24):5360-5364
The use of gem-diborylalkenes as radical-reactive groups is explored for the first time. These reactions provide an efficient and general method for the photochemical conversion of gem-diborylalkenes to rapidly access 1,1-bisborylalkanes. This method exploits a novel photoredox decarboxylative radical addition to gem-diborylalkenes to afford α-gem-diboryl carbon-centered radicals, which benefit from additional stability by virtue of an interaction with the empty p-orbitals on borons. The reaction offers a highly modular and regioselective approach to γ-amino gem-diborylalkanes. Furthermore, EPR spectroscopy and DFT calculations have provided insight into the radical mechanism underlying the photochemistry reaction and the stability of the bis-metalated radicals, respectively. 相似文献
66.
67.
Danieli B Giovanelli P Lesma G Passarella D Sacchetti A Silvani A 《Journal of combinatorial chemistry》2005,7(3):458-462
A library of biologically relevant 6-hydroxy-tetrahydro-beta-carbolines (6-OH-THBCs) based on the L-5-OH-tryptophan scaffold was prepared. A solid-phase synthesis was developed, utilizing aminomethyl polystyrene resin and solid-phase-optimized reactions, such as Pictet-Spengler condensation. The library was designed such that three points of diversity would be readily introduced, making the strategy potentially suitable for generation of a large number of compounds. 相似文献
68.
Charge transport in photofunctional nanoparticles self-assembled from zinc 5,10,15,20-tetrakis(perylenediimide)porphyrin building blocks 总被引:3,自引:0,他引:3
van der Boom T Hayes RT Zhao Y Bushard PJ Weiss EA Wasielewski MR 《Journal of the American Chemical Society》2002,124(32):9582-9590
Molecules designed to carry out photochemical energy conversion typically employ several sequential electron transfers, as do photosynthetic proteins. Yet, these molecules typically do not achieve the extensive charge transport characteristic of semiconductor devices. We have prepared a large molecule in which four perylene-3,4:9,10-tetracarboxydiimide (PDI) molecules that both collect photons and accept electrons are attached to a central zinc 5,10,15,20-tetraphenylporphyrin (ZnTPP) electron donor. This molecule self-assembles into ordered nanoparticles both in solution and in the solid-state, driven by van der Waals stacking of the PDI molecules. Photoexcitation of the nanoparticles results in quantitative charge separation in 3.2 ps to form ZnTPP(+)PDI(-) radical ion pairs, in which the radical anion rapidly migrates to PDI molecules that are, on average, 21 A away, as evidenced by magnetic field effects on the yield of the PDI triplet state that results from radical ion pair recombination. These nanoparticles exhibit charge transport properties that combine important features from both photosynthetic and semiconductor photoconversion systems. 相似文献
69.
70.
The effect of a lanthanide shift reagent Eu(dpm)3 on the spectra of 3 diazacyclophanes (Ia-c) has been studied together with the effect of a fluorinated shift reagent En(fod)3d27 on the nmr spectra of three dithiacyclophanes (IIa-c). The shift reagents remove certain unexpected accidental degeneracies in the original spectra and permit the assignment of the peaks in the expanded spectra to the individual methylene groups. The comparison of the chemical shifts for the methylene groups above the aromatic rings with those of related compounds provides evidence of an upfield shift deriving from the aromatic ring current. The synthesis of two new dithiacyclophanes (IIb-c) is reported. 相似文献