Piecewise Continuous Almost Automorphic Functions and Favard’s Theorems for Impulsive Differential Equations in Honor of Russell Johnson

Journal of Dynamics and Differential Equations - We define piecewise continuous almost automorphic (p.c.a.a.) functions in the manners of Bochner, Bohr and Levitan, respectively, to describe almost...     

基于LPC2210和SSD1339的PM-OLED驱动控制电路

为了避免早期无源矩阵有机电致发光器件驱动控制电路的一些缺陷,如:"串扰"和"交叉"效应以及电路连接比较复杂,用两种方法实现了基于飞利浦公司生产的LPC2210控制芯片和晶门科技公司的SSD1339驱动芯片的驱动控制电路。首先介绍了有机电致发光器件的结构和发光原理以及芯片SSD1339和LPC2210的主要的特点;分析和比较了SSD1339的8080系列并行口和LPC2210外部存储控制单元的读写时序;分别利用LPC2210的通用输入输出单元和外部存储控制单元,成功的控制SSD1339驱动128RGB×128点阵有机电致发光屏。实验结果表明:两种方法不仅可以有效地克服早期驱动控制电路的缺陷,而且可以使有机电致发光屏显示出高质量的图片;用外部存储控制单元实现的驱动控制电路,可以实现约80 Hz的驱动帧频;而使用通用输入输出单元实现的驱动控制电路,可以单步跟踪数据的传输,因此它具有方便查错的优势。本次实验为在不同的集成环境下无源矩阵有机电致发光器件驱动控制电路的设计奠定基础。     

Discussion of the protein characterization techniques used in the identification of membrane protein targets corresponding to tumor cell aptamers

Aptamer is an oligonucleotide chain with specific binding ability to protein and other targets,which is widely used in ma ny fields.Because of its ability to screen the premise of unknown targets,it can be used to discover some novel tumor markers,i.e.,membrane proteins that are specifically highly expressed on the surface of tumor cells.Tumor markers can be used in many fields such as early diagnosis and treatment,and a new type of tumor marker proved to be effective can significantly improve the therapeutic effect of such tumors.However,further characterization of newly acquired membrane proteins is essential for their clinical use as tumor markers.This review first briefly introduced the process of obtaining novel tumor markers from nucleic acid aptamers.Next,the commonly used protein characterization methods could be used as a technical means to identify membrane protein targets corresponding to tumor cell aptamers,to clarify the principles,advantages and disadvantages of various means,and to analyze the most suitable situations for various experimental methods.Finally,the outlook was made and the characterization methods that should be used in such experiments were summarized.     

Active Control of Thermo-mechanical Buckling of Composite Laminated Plates Using Piezoelectric Actuators

This paper is concerned with the active control of thermo-mechanical buckling of composite laminated plates using piezoelectric facesheets as actuators.The four...     

动态法测量材料的磁电效应

根据动态法测量磁电效应的基本原理,优化设计并建立了磁电效应测量系统,对其中的一些重要参数对磁电效应测量的影响进行了研究.结果表明,综合考虑测量需要,选择适当的测量参数,对于磁电效应的正确测量十分重要.适当增加线圈长度、减小线圈匝数以及减小交变磁场场强,都是提高磁电效应测量精度、减小测量误差的有效途径.     

高效液相色谱-柱后衍生和质谱联用测定大米中氨基甲酸酯类农药残留量

研究了高效液相色谱-柱后衍生荧光检测和液质联用分别测定大米中14种和16种氨基甲酸酯类农药残留量的方法.二氯甲烷和丙酮的混和溶剂提取后,用柱后衍生荧光检测法和离子阱多级质谱联用法进行测定.柱后衍生荧光检测法检出限为20～50 μg/kg,离子阱多级质谱联用法检出限为10～20 μg/kg,添加平均回收率在70%～92%之间,RSD%在6.7%～12%.     

Multiplex metal-detection based assay (MMDA) for COVID-19 diagnosis and identification of disease severity biomarkers

The ongoing COVID-19 pandemic caused by SARS-CoV-2 highlights the urgent need to develop sensitive methods for diagnosis and prognosis. To achieve this, multidimensional detection of SARS-CoV-2 related parameters including virus loads, immune response, and inflammation factors is crucial. Herein, by using metal-tagged antibodies as reporting probes, we developed a multiplex metal-detection based assay (MMDA) method as a general multiplex assay strategy for biofluids. This strategy provides extremely high multiplexing capability (theoretically over 100) compared with other reported biofluid assay methods. As a proof-of-concept, MMDA was used for serologic profiling of anti-SARS-CoV-2 antibodies. The MMDA exhibits significantly higher sensitivity and specificity than ELISA for the detection of anti-SARS-CoV-2 antibodies. By integrating the high dimensional data exploration/visualization tool (tSNE) and machine learning algorithms with in-depth analysis of multiplex data, we classified COVID-19 patients into different subgroups based on their distinct antibody landscape. We unbiasedly identified anti-SARS-CoV-2-nucleocapsid IgG and IgA as the most potently induced types of antibodies for COVID-19 diagnosis, and anti-SARS-CoV-2-spike IgA as a biomarker for disease severity stratification. MMDA represents a more accurate method for the diagnosis and disease severity stratification of the ongoing COVID-19 pandemic, as well as for biomarker discovery of other diseases.

A MMDA platform is developed by using metal-tagged antibodies as reporting probes combined with machine learning algorithms, as a general strategy for highly multiplexed biofluid assay.     

高效液相色谱法测定动物组织中鱼腥草素同系物含量

以氢氧化钠水解合成鱼腥草素类药物,然后用乙醚提取,除去乙醚后,用HPLC法测定大鼠血样和组织样品中药物的含量。结果表明:碱水解后合成鱼腥草素,形成共轭双键的烯醇式烷酰乙醛,紫外吸收显著增强,HPLC图谱测定物峰分离良好。血液加样标准曲线回归线性良好,r=0.999以上,线性范围为0.1-100mg/L,标准加入回收率为97.3%。利用该方法分析合成鱼腥草素类药物在动物血液、脑、肝、心脏、肾脏和肌肉等组织中的残留完全可行。     

石墨炉原子吸收直接测定蜂胶中铅

1引言蜂胶是蜜蜂从植物幼芽及树干上采集的树脂并混入蜜蜂上腭腺分泌物和蜂蜡等形成的具有粘性的天然胶状物质。蜂胶化学成分复杂,其中主要有黄酮类化合物、酚酸、酯、脂肪酸等化合物,由于蜂胶临床上具有显著抗菌、抗癌、提高机体免疫力等医疗保健作用,在国际上被誉为“紫黄金”。但蜂胶中重金属Pb一直为人们所关心,蜂胶中Pb可能来源于大气、提取及加工过程中。通常检测Pb要先对样品进行消化,本实验研究一种快速、简便和准确的测定蜂胶中Pb的方法,用无水乙醇溶解蜂胶直接用于石墨炉原子吸收测定并与国家标准方法比较测定,结果满意。2实验…     

固相萃取-离子色谱-积分脉冲安培法测定人血清中游离氨基酸

比较了磺基水杨酸和无水甲醇沉淀人血清中蛋白质的效果,优化了国产732型阳离子交换树脂进行固相萃取净化的条件和Dionex-600离子色谱分析系统的梯度淋洗条件。用该离子色谱仪检测了谷氨酰胺等14种氨基酸,结果表明,峰面积-浓度的校正曲线的相关系数为0.9900~0.9995,检出限为0.02~0.59mg/L;峰面积的相对标准偏差为1.9%~10.4%;回收率为80.6%~114.8%。