Forced Degradation Study on Gliclazide and Application of Validated Stability-Indicating HPLC-UV Method in Stability Testing of Gliclazide Tablets

Forced degradation study on gliclazide was conducted under the conditions of hydrolysis, oxidation, dry heat and photolysis and an isocratic stability-indicating HPLC-UV method was developed and validated. All the seven degradation products (I–VII) formed under different conditions were optimally resolved on a C₁₈ column with mobile phase composed of 40% acetonitrile and 60% ammonium acetate solution (0.025 M, pH 3.5) at a flow rate of 0.25 mL min^?1 using 235 nm as detection wavelength. The method was linear between 5–500 μg mL^?1 drug concentrations. The %RSD of intra- and inter-day precision studies was <1 and <2% respectively. Excellent recoveries (99.81–100.97%) proved the method sufficiently accurate. Each peak resolved always with a resolution of >1.90 indicating the method to be rugged enough. The method was used to study the drug degradation behaviour under the forced conditions. Four degradation products (I–IV) were formed in 0.1 N HCl and water whereas only I and III were formed in 3% H₂O₂. Two new products V and VI in addition to I, III and IV were formed in 0.1 N NaOH. The drug was stable to thermal and photolytic decomposition. The degradation behaviour in water and 0.1 N NaOH was similar under dark and light conditions but a new product VII was formed in 0.01 N HCl in light. In general, the rate of degradation was accelerated by the light. The method was applied successfully in stability testing of gliclazide tablets.     

Selective filling for patterning in microfluidic channels

The ability to pattern different polymers in microfluidic channels is indispensable for the development of multifunctional, "lab-on-a-chip" devices. A simple method, based on the concept of selective filling, is described for introducing different polymers at defined locations in microfluidic channels. Selective filling is based on the difference in the free energy of filling between an open and a covered part of the channel. It is implemented by covering part of the channel, along its length, with a temporary poly(dimethylsiloxane) (PDMS) slab. Preferential filling is related to the contact angle of the liquid solution on the chip surface. An expression for the critical contact angle is derived, and its dependence on the geometry of the channel is established. It is further shown that a trapezoidal geometry of the cross-section of the channel is optimal for selective filling. Experimental verification of the applicability of the critical contact angle in predicting selective filling is demonstrated by introducing liquid prepolymer solutions of different contact angles in the glass channel that was etched using photolithography and wet etching. Finally, patterning of two different polymers along the axial direction of the microfluidic channel is demonstrated using this selective filling technique.     

Formation of Dimers of Some 2‐Substituted Indan‐1‐one Derivatives during Base‐Mediated Cross‐Aldol Condensation

Unexpected dimers of some 2‐substituted indan‐1‐one derivatives were isolated during aldol condensation of indan‐1‐one with various aldehydes in the presence of KOH (see Scheme). Monomeric products, usually expected from aldol condensation, further underwent a base‐catalyzed nucleophilic addition reaction to their dimeric form in some cases. The structures of these dimers were characterized by using various spectral techniques and in one case, structural details were determined from a high‐resolution crystallographic analysis.     

Studies of the thiadiazine, taurolidine-I. Identification of the molecular species present in aqueous solutions by (1)H- and (13)C-NMR spectroscopy

The equilibria in deuterium oxide solutions of the diamine, 4,4'-methylenebis(tetrahydro-1,2,4-thiadiazine-1,1-dioxide), were studied using highfield (1)H- and (13)C-NMR with the aid of solutions of tetrahydro-2H-1,2,4-thiadiazine-1,1-dioxide (taurultam), its two N-methyl detivatives and methylene glycol. Comparison of the (1)H-NMR spectrum of taurolidine with the one obtained from a mixture of taurultam and methylene glycol indicated that the same equilibria exists in both these solutions. It was concluded that taurolidine, taurultam and its 4-hydroxymethyl adduct and methylene glycol are the major components present. To facilitate the interpretation of the (13)C-spectra, (13)C-enriched methylene glycol was added to solutions of taurultam. The (13)C-studies confirmed the (1)H-NMR study.     

Why Is CO2 so soluble in imidazolium-based ionic liquids?

Experimental and molecular modeling studies are conducted to investigate the underlying mechanisms for the high solubility of CO2 in imidazolium-based ionic liquids. CO2 absorption isotherms at 10, 25, and 50 degrees C are reported for six different ionic liquids formed by pairing three different anions with two cations that differ only in the nature of the "acidic" site at the 2-position on the imidazolium ring. Molecular dynamics simulations of these two cations paired with hexafluorophosphate in the pure state and mixed with CO2 are also described. Both the experimental and the simulation results indicate that the anion has the greatest impact on the solubility of CO2. Experimentally, it is found that the bis(trifluoromethylsulfonyl)imide anion has the greatest affinity for CO2, while there is little difference in CO2 solubility between ionic liquids having the tetrafluoroborate or hexafluorophosphate anion. The simulations show strong organization of CO2 about hexafluorophosphate anions, but only small differences in CO2 structure about the different cations. This is consistent with the experimental finding that, for a given anion, there are only small differences in CO2 solubility for the two cations. Computed and measured densities, partial molar volumes, and thermal expansion coefficients are also reported.     

Inclusive and exclusive measurements in the projectile breakup of 7Li

The inclusive and exclusive measurements were carried out for ⁷Li projectile breakup on ²⁷Al target at 48 MeV. In the inclusive data we have observed a broad peak around the beam velocity for alphas and tritons. The exclusive data for alpha-triton coincidences show good agreement with the post-form DWBA theory of breakup reactions.     

Kobalt und Nickel

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