HPLC法测定利福昔明干混悬剂的含量

采用Kromasil-C8色谱柱(250×4.6mm,5μm),流动相为甲醇-0.075mol/L磷酸二氢钾溶液-1.0mol/L枸橼酸溶液(60:26:4),检测波长254nm,HPLC法测定利福昔明干混悬剂含量.利福昔明干混悬剂在0.048-0.160(mg/mL)浓度范围内,峰面积与浓度呈良好的线性关系(r=0.9999),平均回收率为99.9%.本法简便,专属性及重复性好,可用于利福昔明干混悬剂的含量测定.     

Dynamic synovial fibroblasts are modulated by NBCn1 as a potential target in rheumatoid arthritis

Rheumatoid arthritis (RA) is an autoimmune disease characterized by aggressive fibroblast-like synoviocytes (FLSs) and pannus formation. Various therapeutic strategies have been developed against inflammatory cytokines in RA in recent decades. Based on the migratory features of FLSs, we examined whether modulation of the migratory module attenuates RA severity. In this study, inflamed synovial fluid-stimulated FLSs exhibited enhanced migration and migratory apparatus expression, and sodium bicarbonate cotransporter n1 (NBCn1) was identified in primary cultured RA-FLSs for the first time. The NBC inhibitor S0859 attenuated the migration of FLSs induced with synovial fluid from patients with RA or with TNF-α stimulation. Inhibition of NBCs with S0859 in a collagen-induced arthritis (CIA) mouse model reduced joint swelling and destruction without blood, hepatic, or renal toxicity. Primary FLSs isolated from the CIA-induced mouse model also showed reduced migration in the presence of S0859. Our results suggest that inflammatory mediators in synovial fluid, including TNF-α, recruit NBCn1 to the plasma membrane of FLSs to provide dynamic properties and that modulation of NBCn1 could be developed into a therapeutic strategy for RA.Subject terms: Chemotaxis, Bone, Ion channel signalling, Rheumatoid arthritis, Drug development     

高压静电场促进植物生长技术的研究

高压静电场促进植物生长技术源于自然又高于自然 ,它包括静电种子处理和静电场生长环境等技术 .文章论述了该技术的实施方法 ,并报道了已经取得的初步成果 .     

CeO_2和Y_2Ba_1Cu_1O_(5-δ)共同掺杂对Y_1Ba_2Cu_3O_(7-δ)的超导电性及其晶格结构的影响

采用固相反应法成功制备出一批Y123与Y211的摩尔比为1∶0.47的混合物,并在此混合物的基础上掺入CeO2,掺入的比例x分别为0.5wt%、1.0wt%、2.0wt%、3.0wt%、4.0wt%。采用了X-射线衍射仪对样品的晶格结构进行了分析,测量结果表明:x=1.0wt%时掺杂效果最好,同时也充分说明Y123的晶格结构与其超导电性之间存在着一种内在的必然关联。并通过对其测试临界转变温度以及转变宽度,测量结果也充分验证了上述结论。     

国家类型学说在近代东亚的传播与重构

国家类型学说源于欧美,是关于如何系统地区分和比较不同国家形式的学问,19世纪中叶以降伴随着西学东渐的潮流输入东亚文明圈。相较于中国的进程缓慢和规模有限,明治日本迅速形成了一套内涵丰富的以辨析国体政体关系为主旨的国家类型知识体系。国体政体是否有别、如何区分的争论,虽直接导源于西方的国家形式、政府形式异同问题,但根本症结在于日本以天皇总揽统治权之“体”行立宪政治之“用”后面临君权与民权的纠缠。清季朝野各方从日本引进各类国体政体理论探索改制问题,同样因君权、民权取向的互歧而衍生出彼此互异的国体政体观念。辛亥革命后,因缘君权时代的结束和民主共和的兴起,日本的国体政体学说在中国日趋失去活力,而民初议会政治的破产和主权所有者“民”背后阶级问题的出现,预示着新兴革命力量必须提出真正符合中国新的政治形势和需求的国家类型理论。     

Diels–Alder dimerization of Morita–Baylis–Hillman acetates catalyzed by organocatalysts

DABCO-catalyzed dimerization of Morita–Baylis–Hillman acetates to synthesize a series of 3-alkyl-4-(E)-alkenyl-cyclohex-1-ene-1,4-dicarbonyl compounds in excellent yields with modest to excellent diastereoselectivity is reported. A plausible reaction mechanism is also proposed on the basis of previous literature and preliminary investigation the asymmetric version of the reaction.     

晶态硅中氢化单空位的络合物模型

本文对氢化单空位模型中SiH键间的相互作用对总能量的影响进行了计算。据用全略微分重迭(CNDO/2)方法计算的结果,单空位中四个氢原子有较明显的相互作用,四氢原子络合成组是含氢单晶硅中一种合理的氢相关组态模型。 关键词：