Model-based sensitivity analysis of a fluidised-bed bioreactor for mercury uptake by immobilised Pseudomonas putida cells

A model-based sensitivity analysis was performed in order to evaluate the importance of the individual operating parameters of a three-phase fluidised-bed biological reactor used for removing mercury ions from wastewater. The parameters analysed involve the immobilised biomass load (bacteria P. putida) on alginate beads, particle size, inlet flow-rate, mercury ion loads in the fed wastewater, and the solid fraction in the reactor. Predictions were generated by using pseudo-first-order, Michaelis-Menten, or pseudo-Haldane kinetic models. The results highlight the major influence of the biomass/solid load and of the liquid residence time on the reactor efficiency. Also, the resultant significant differences in the model predictions underline the importance of using a more accurate kinetic model for process design and control purposes.     

Electrochemical Behaviour and Square Wave Voltammetry of the Rice Herbicides Molinate,Bensulfuron-Methyl,Mefenacet and Thiobencarb

Abstract

Cyclic, differential pulse and square wave voltammetry were applied to develop an electroanalytical procedure for the determination of a group of herbicides used to treat rice crops: molinate, bensul-ruron-methyl, mefenacet and thiobencarb. The oxidation mechanism causes adsorption problems and consequent poisoning of the electrode surface by the products of the electrochemical reaction. Parameters such as pH, frequency and electrochemical electrode surface treatment were optimized. The analytical methodology developed using square wave voltammetry was applied to the determination of molinate and bensulfuron-methyl in simple or mixed commercial products.     

Ancient silver extraction in the Montevecchio mine basin (Sardinia, Italy): micro-chemical study of pyrometallurgical materials

Different pyrometallurgical materials such as slags, refractory materials and thermally treated lead ores likely related to smelting and extractive processes and chronologically related to Punic and Roman periods (IV–III BC) have been found at Bocche di Sciria and Conca e Mosu in the Montevecchio mine basin (south western Sardinia, Italy), where archaeological findings and classical authors locate extractive metallurgy activities since pre-Roman times. By means of the combined use of X-ray diffraction (XRD), scanning electron microscopy (SEM) combined with energy-dispersive X-ray spectrometry (EDS), selected-area X-ray photoelectron spectroscopy (XPS) and optical microscopy (OM), micro-chemical and micro-structural investigations have been carried out in order to identify the nature of the pyrometallurgical materials, to decipher the processes carried out there and their technological steps and to determine the technological level of competence reached by the ancient metallurgists. The results confirm that the findings can be associated with smelting and extractive processes carried out close to the metal ore deposits first for the argentiferous lead production and, then, for the silver recovery via a cupellation process. Finally, the results disclose the high level of technological competence of the ancient metallurgists able to carry out complex high-temperature processes to treat the argentiferous lead ores and to recover low amounts of silver via high-temperature lead-selective oxidation.     

A theoretical investigation of the mechanism of formation of a simplified analog of the green fluorescent protein (GFP) from a peptide model

Theoretical calculations at the B3LYP/6-311++G(d,p) level have been carried out on the reaction path connecting a dipeptide to an imidazolinone as a model for the formation of GFP. In addition, we have studied the hydration effects on the processes, adding a water molecule to assist the cyclization. The solvent effects have been taken into account by introducing the monohydrated molecules into a solvent cavity with a polarized continuum model. Significant reductions of the energy barriers for the reaction path can be observed within the water-assisted processes. The solvent effects account for a barrier lowering of 4–5 kJ mol^?1.     

Do halogen and methyl substituents have electronic effects on the structures of simple disilanes? An experimental and theoretical study of the molecular structures of the series X3SiSiMe3 (X = H, F, Cl and Br)

The gas-phase molecular structures of a series of halogen-substituted disilanes [X₃SiSiMe₃ (X = H, F, Cl and Br)], 1,1,1-trimethyldisilane (H₃SiSiMe₃), 1,1,1-trifluoro-2,2,2-trimethyldisilane (F₃SiSiMe₃), 1,1,1-trichloro-2,2,2-trimethyldisilane (Cl₃SiSiMe₃) and 1,1,1-tribromo-2,2,2-trimethyldisilane (Br₃SiSiMe₃), have been determined in the gas phase by electron diffraction. Ab initio calculations at the HF and MP2 level were used to support the experimental investigation using the SARACEN method. All of the investigated structures were determined to adopt a staggered structure with C _3v symmetry. The effect of substitution on the Si–Si bond and the Si–Si–X bond angle was investigated and these results were compared to results obtained from a recent study of halogen-substituted disilanes [X₃SiSiXMe₂ (X = F, Cl, Br and I)] to consider the effect of the methyl groups on the substituted disilanes.     

π-Bridge Substitution in DASAs: The Subtle Equilibrium between Photochemical Improvements and Thermal Control**

Donor–acceptor Stenhouse adducts (DASAs) are playing an outstanding role as innovative and versatile photoswitches. Until now, all the efforts have been spent on modifying the donor and acceptor moieties to modulate the absorption energy and improve the cyclization and reversion kinetics. However, there is a strong dependence on specific structural modifications and a lack of predictive behavior, mostly owing to the complex photoswitching mechanism. Here, by means of a combined experimental and theoretical study, the effect of chemical modification of the π-bridge linking the donor and acceptor moieties is systematically explored, revealing the significant impact on the absorption, photocyclization, and relative stability of the open form. In particular, a position along the π-bridge is found to be the most suited to redshift the absorption while preserving the cyclization. However, thermal back-reaction to the initial isomer is blocked. These effects are explained in terms of an increased acceptor capability offered by the π-bridge substituent that can be modulated. This strategy opens the path toward derivatives with infra-red absorption and a potential anchoring point for further functionalization.