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91.
Removal of 2,4-dichlorophenoxyacetic acid by calcined Zn-Al-Zr layered double hydroxide 总被引:2,自引:0,他引:2
The adsorption equilibrium, kinetics, and thermodynamics of removal of 2,4-dichlorophenoxy-acetic acid (2,4-D) from aqueous solutions by a calcined Zn-Al layered double hydroxide incorporated with Zr(4+) were studied with respect to time, temperature, pH, and initial 2,4-D concentration. Zr(4+) incorporation into the LDH was used to enhance 2,4-D uptake by creating higher positive charges and surface/layer modification of the adsorbent. The LDH was capable of removing up to 98% of 2,4-D from 5 to 400 ppm aqueous at adsorbent dosages of 500 and 5000 mg L(-1). The adsorption was described by a Langmuir-type isotherm. The percentage 2,4-D removed was directly proportional to the adsorbent dosage and was optimized with 8% Zr(4+) ion content, relative to the total metals (Zr(4+)+Al(3+)+Zn(2+)). Selected mass transfer and kinetic models were applied to the experimental data to examine uptake mechanism. The boundary layer and intra-particle diffusion played important roles in the adsorption mechanisms of 2,4-D, and the kinetics followed a pseudo-second order kinetic model with an enthalpy, ΔH(ads) of -27.7±0.9 kJ mol(-1). Regeneration studies showed a 6% reduction in 2,4-D uptake capacity over six adsorption-desorption cycles when exposed to an analyte concentration of 100 ppm. 相似文献
92.
Enzyme immunoassay for semicarbazide--the nitrofuran metabolite and food contaminant 总被引:4,自引:0,他引:4
Semicarbazide (SEM), the marker residue for the banned nitrofuran veterinary antibiotic nitrofurazone (NFZ), has been detected regularly in foods (47% of recent nitrofuran EU Rapid Alerts involve SEM). However, the validity of SEM as a definitive marker for NFZ has been undermined by SEM arising from other sources including azodicarbonamide, a plastics blowing agent and flour treatment additive. An inexpensive screening test for SEM in food matrices is needed—all SEM testing currently uses expensive LC-MS/MS instrumentation. We now report the first production of antibodies against derivatised SEM. A novel carboxyphenyl SEM derivative was used to raise a polyclonal antibody that has been incorporated into a semi-quantitative microtitre plate ELISA, validated according to the criteria set out in Commission Decision 2002/657/EC, for use with chicken muscle. The antibody is highly specific for derivatised SEM, cross-reactivity being 1.7% with NFZ and negligible with a wide range of other nitrofurans and poultry drugs. Samples are derivatised with o-nitrobenzaldehyde and simultaneously protease digested before extraction by cation exchange SPE. The ELISA has a SEM detection capability (CCβ) of 0.25 μg kg−1 when a threshold of 0.21 μg kg−1 is applied to the selection of samples for confirmation (lowest observed 0.25 μg kg−1 fortified sample, n = 20), thus satisfying the EU nitrofurans’ minimum required performance limit of 1 μg kg−1. NFZ-incurred muscles (12) containing SEM at 0.5-5.0 μg kg−1 by LC-MS/MS, all screened positive by this ELISA protocol which is also applicable to egg and chicken liver. 相似文献
93.
94.
Jeanne Brygier Michel Gelbcke Claude Guermant Michelle Nijs Danielle Baeyens-Volant Yvan Looze 《Applied biochemistry and biotechnology》1993,42(2-3):127-135
Carbonylimidazol-l-yl-mPEG is obtained quantitatively by reacting methoxypoly(ethyleneglycol) (mPEG) with 1.1 Eq of N,N′-carbonyldiimidazole
in the presence of a stoechiometric amount of 4-dimethyl-aminopyridine used as hypernucleophilic acylation catalyst. Carbonylimidazol-l-yl-mPEG is quite stable in aqueous solutions with half-lives up to 70 h in pHs ranging from 6.0 to 7.0 at 25‡C. From reactivity
studies toward amines with various nucleophilic strengths, it is suggested that carbonylimidazol-l-yl-mPEG may be best used to modify α-amino terminal function of proteins selectively or to introduce amino function on PEG chains. 相似文献
95.
This paper presents a partial classification for C∞ type-changing symplectic Monge-Ampère partial differential equations (PDEs) that possess an infinite set of first-order intermediate PDEs. The normal forms will be quasi-linear evolution equations whose types change from hyperbolic to either parabolic or to zero. The zero points can be viewed as analogous to singular points in ordinary differential equations. In some cases, intermediate PDEs can be used to establish existence of solutions for ill-posed initial value problems. 相似文献
96.
Kohut-Svelko N Reynaud S Dedryvère R Martinez H Gonbeau D François J 《Langmuir : the ACS journal of surfaces and colloids》2005,21(4):1575-1583
A simple way to obtain a conducting nanocomposite is described, and the conducting particles are characterized. Core-shell particles [polystyrene-polyaniline (PANI)] have been obtained by the dispersion process from three types of polystyrene latexes: a no-cross-linked core stabilized by a nonylphenolethoxylate (NP40) and two cross-linked cores stabilized by NP40 and a mixture NP40/Surfamid (a surfactant bearing an amide group). The surface of these particles has been extensively characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy, and scanning electron microscopy. A maximum coverage of 94% was obtained for the high PANI content as revealed by XPS analysis. A better coverage was obtained for the cross-linked polystyrene latex stabilized by the Surfamid. The amide group of this surfactant allows the H-bonding formation with the PANI backbone and, thus, improves the conductivity. It was shown that a uniform coverage of the core particles was not required to ensure a good conductivity. 相似文献
97.
Conformational changes of proteins modulate their function. In allosteric control, the conformational change is induced by the binding of a signaling molecule. Here we insert a "molecular spring" on the enzyme guanylate kinase, to control the conformation of this protein. The stiffness of the spring can be varied externally, which allows one to exert a controlled mechanical tension between the two points on the protein's surface where the spring is attached. We show that by applying and releasing the tension we can reversibly turn the enzyme off and on. 相似文献
98.
We demonstrate the allosteric control of a protein based on mechanical tension. When substrate binding is accompanied by a significant change of conformation of the protein, a mechanical tension favoring one or the other conformation will alter the binding affinity for the substrate. We have constructed a chimera where the two lobes of the maltose-binding protein are covalently coupled to the ends of a DNA oligomer. The mechanical tension on the protein is controlled externally by exploiting the difference in stiffness between single stranded and double stranded DNA. We report that the binding affinity of the protein for its substrates is significantly altered by the tension. 相似文献
99.
100.
Ronald Lafrance Jean-Pierre Aycard Jeanne Berger Hubert Bodot 《Magnetic resonance in chemistry : MRC》1976,8(2):95-101
The 1H NMR spectra of a series of cis and trans-3R,4 X-cyclohexanones (-2,2,6,6-d4) are analysed. By comparison of their 3J coupling constants with those of cyclohexane homologues we obtain information about the chair–chair equilibrium constants for R = CH3, X = CN, the chair structure of cis isomers with an equatorial t-butyl group, and a conformational heterogeneity with trans (CH3)3C and CN groups. This latter situation is analysed by means of a simplified but controlled Karplus relationship, on the basis of a mixture of two conformers; this involves a diequatorial chair and a boat form with a dihedral angle Φ34 of about ?6°. 相似文献