The degree of C?C bond activation in the asymmetric bioreduction of α,β-unsaturated carboxylic esters by ene-reductases was studied, and general recommendations to render these "borderline-substrates" more reactive towards enzymatic reduction are proposed. The concept of "supported substrate activation" was developed. In general, an additional α-halogenated substituent proved to be beneficial for enzymatic activity, whereas β-alkyl or β-aryl substituents were detrimental for the reactivity of nonhalogenated substrates, and α-cyano groups showed little effect. The alcohol moiety of the ester functionality was found to have a strong influence on the reaction rate. Overall, activities were determined by both steric and electronic effects. 相似文献
A class of cationic bottle‐brush polymers that show ionic strength‐dependent stimuli responsiveness is prepared. Brush polymers with norbornene as backbone and quaternary ammonium (QA)‐containing polycaprolactone copolymers as side chains are synthesized by a combination of ring‐opening metathesis polymerization, ring‐opening polymerization, and click reaction. In water with low ionic strength, brush polymers are soluble due to the strong electrostatic repulsion between cationic QA groups. As the addition of salt to increase ionic strength, single brush polymers undergo a transition from extended conformation to collapsed state and finally become insoluble in solution due to the screening effect of salts that yield the once‐dominant electrostatic interactions among QA species to hydrophobic–hydrophobic interactions.
Often the rate of passage of gaseous molecules through model zeolites is too small to be computed directly. An estimate for the rate of passage of CCl4 through the 8-ring window in a model of zeolite A has been obtained by combining a direct evaluation of the free energy profile and an adaptation of the rare events method. First the free energy profile is found from a direct evaluation of the canonical partition function at high dilution and the transition state theory rate constant obtained. The dynamic correction factor is then estimated from molecular dynamics runs and used to compute the actual rate keff. The method is used to estimate the rate of passage through the 8-ring window in a rigid model of zeolite A, and the results are compared with those obtained from rigid models with expanded windows and from the flexible model. Even a small expansion in the 8-ring window diameter increases the rate significantly, but the changes associated with a flexible cage are small. 相似文献
We study the BPS spectra of ${\mathcal{N}=2}$ N = 2 complete quantum field theories in four dimensions. For examples that can be described by a pair of M5 branes on a punctured Riemann surface we explain how triangulations of the surface fix a BPS quiver and superpotential for the theory. The BPS spectrum can then be determined by solving the quantum mechanics problem encoded by the quiver. By analyzing the structure of this quantum mechanics we show that all asymptotically free examples, Argyres-Douglas models, and theories defined by punctured spheres and tori have a chamber with finitely many BPS states. In all such cases we determine the spectrum. 相似文献
FLASH at DESY in Hamburg, the first and currently only free-electron laser for VUV and soft X-ray radiation, started user operation in the summer of 2005. Currently it covers a wavelength range from 13 nm to 50 nm with GW peak power and pulse durations between 10 fs and 50 fs. Approximately 20 weeks of beamtime are provided per year for scientific experiments that are not possible on any other radiation source. 相似文献
Pure samples of cis,cis- and trans,trans-1,4-difluorobutadiene-2-d1 have been synthesized, and high-resolution (0.0015 cm?1) infrared spectra have been recorded for these nonpolar molecules in the gas phase. For the cis,cis isomer, the rotational structure in two C-type bands at 775 and 666 cm?1 and one A-type band at 866 cm?1 has been analyzed to yield a combined set of 2020 ground state combination differences (GSCDs). Ground state rotational constants fit to these GSCDs are A0 = 0.4195790(4), B0 = 0.0536508(8), and C0 = 0.0475802(9) cm?1. For the trans,trans isomer, three C-type bands at 856, 839, and 709 cm?1 have been investigated to give a combined set of 1624 GSCDs. Resulting ground state rotational constants for this isomer are A0 = 0.9390117(8), B0 = 0.0389225(4), and C0 = 0.0373778(3) cm?1. Small inertial defects confirm the planarity of both isomers in the ground state. Upper state rotational constants have been determined for most of the transitions. The ground state rotational constants for the two isotopologues will contribute to the data set needed for determining semiexperimental equilibrium structures for the nonpolar isomers of 1,4-difluorobutadiene. 相似文献
Summary Measurements have been made of the electron lateral-distribution age parameter for cosmic-ray showers with sea-level sizes
from ∼105 to 6·106 particles. It is found that, in the size range from ∼6·105 particles to ∼6·106 particles, the shower age decreases rapidly with size corresponding to a rapid change in the equivalent development depth
with shower size. Measurements of the age distribution of the showers with sizes below ∼5·105 show a selection bias towards young showers.
Riassunto Sono state fatte misurazioni del parametro di età della distribuzione laterale degli elettroni per sciami di raggi cosmici
con grandezza a livello del mare da ∼105 a 6·106 particelle. Si è trovato che, nell’intervallo di grandezza da ∼6·105 a ∼6·106 particelle, l’età dello sciame diminuisce rapidamente con la grandezza in corrispondenza di un rapido cambiamento della profondità
di sviluppo equivalente con la grandezza dello sciame. Le misurazioni della distribuzione in età degli sciami con grandezze
al di sotto di ∼5·105 mostrano un errore sistematico di selezione verso gli sciami giovani.
Резюме Были проведены измерения параметра распределения возраста для ливней космических лучей с размерами на уровне моря от ∼105 до 6·106 частиц. Получено, что в области размеров от ∼6·105 частиц до ∼6·106 частиц возраст ливней быстро уменьшается с размером, соответствующим быстрому изменению в эквивалентной глубине развития
с размером ливня. Измерения распределения возрастов ливней с размерами ниже ∼5·105 обнаруживают селективное смещение в сторону ?молодых? ливней.
Photosensitization mediated by Photofrin II (PFII) was found to be mutagenic at the heterozygous thymidine kinase (tk) locus in mouse L5178Y lymphoma strains LY-S1 and LY-R16 but not in strain LY-R83 which is hemizygous at the tk locus. After treatments yielding 37% survival, the mutagenicity of photosensitization with PFII in strain LY-S1 was similar to that of other mutagenic agents including x-radiation, ethyl methanesulfonate, and photosensitization with chloroaluminum phthalocyanine (AlPcCl). Although both strain LY-S1 and strain LY-R16 were mutagenized by photosensitization with PFII, only strain LY-S1 was mutagenized by photosensitization with AlPcCl. The non-mutability of strain LY-R83 following photodynamic treatment with either sensitizer may be because of the poor recovery of mutants with intergenic mutations in this TK+/0 hemizygous strain, whereas the non-mutability of strain LY-R16 subjected to photodynamic treatment with AlPcCl may be because LY-R16 cells sustaining mutagenic damage do not survive for reasons other than the loss of an essential gene. 相似文献
Summary A new method for the calculation of partial cross sections in the time-dependent quantum theory of molecular reactive scattering processes is discussed. Preliminary calculations are presented which clearly illustrate the power of the method. They show how all the partial cross sections associated with a single initial quantum state may be computed over a very wide energy range from a single propagation of a prepared wavepacket. The resonance behaviour is obtained without difficulty and the energies of the reactive scattering resonances are exactly reproduced. 相似文献
Dispiro[3.1.3.1]decan-5,10-dione was selectively difluorinated at room temperature by diethylaminosulfur trifluoride (DAST) to give 10,10-difluorodispiro[3.1.3.1]decan-5-one. The latter was reduced to 5,5-difluorodispiro[3.1.3.1]decane and also reacted with excess DAST to give 5,5,10,10-tetrafluorodispiro[3.1.3.1]decane. Proton and fluorine magnetic resonance studies on 5,5-difluorodispiro[3.1.3.1]decane did not show significant cross-ring coupling (< 0.5 Hz) between geminal fluorines on C-5 and geminal hydrogens on C-10. The fluorine magnetic resonance spectrum of 5,5,10,10-tetrafluorodispiro[3.1.3.1]decane was a single sharp line which establishes that through-space coupling between geminal fluorines at C-5 and C-10 and geminal hydrogens on other carbons does not exist. A study of the infrared and ultraviolet spectra of dispiro[3.1.3.1]-decane-5,10-dione indicate the cross-ring carbonyl groups interact significantly. 相似文献