Improving the precision of fluorescence lifetime measurement using a streak camera

Streak camera has high temporal resolution and high sensitivity, and is a powerful tool in biomedical study to measure fluorescence lifetime and perform fluorescence lifetime imaging. However, nonuniformity of the gain in the streak tube and nonlinearity of the sweeping speed limit the precision of fluorescence lifetime measurement, particularly when fluorescence lifetimes are short. We have constructed a two-photon excitation fluorescence lifetime measurement system that is based on a synchroscan streak camera and have developed accordingly a method to correct the effect of gain nonuniformity and nonlinearity of sweeping speed on the measurement precision. A continuous-wave laser of high stability is used to calibrate the gain of the streak camera, and a Fabry-Perot etalon is used to calibrate the nonlinearity of the sweeping speed. Fitting algorithms are used to correct the gain of the streak camera and nonlinearity of the sweeping speed respectively, which significantly improves the measurement precision of the system, as characterized through the fluorescence lifetime of the short-lived fluorescence dye, Rose Bengal. Experimental results show that the measurement fluctuation of the lifetime has been improved from more than 10% to 2% after correcting the effects of gain nonuniformity and sweeping speed nonlinearity.     

正交小波变换在散斑图象相关计算中的应用

本文在散斑图象的相关计算处理技术中引入了二维离散正交小波变换,详细叙述了其原理,给出了实验计算的结果,并与原始的空域相关计算进行了比较,从中可以看出:无论是相关计算精度,还是相关搜索速度,小波相关都具有明显的优越性.     

多模虚共轭相干态与多模真空态组成的叠加态光场的等阶N次方H压缩

构造了由多模虚共轭相干态|{iZ_j^*}〉_q与多模真空态|{0_j}〉_q两者的线性叠加所组成的两态叠加多模叠加态光场|{Ψ_q⁽²⁾}〉_q.利用多模压缩态理论,首次对该态的等阶N次方H压缩特性进行了详细研究.结果发现:态|{Ψ_q⁽²⁾}〉_q是一种典型的多模非经典光场,它可呈现出周期性变化的、任意阶的等阶N次方H压缩效应.     

一种新型微透镜阵列成象式投影光刻系统的研究

从理论和实验两方面入手,系统地研究了微透镜阵列的形成原理和制作工艺,分析了微透镜阵列的面形结构,得到了联系微透镜阵列矢高与制作工艺参量的经验公式.并且将四片微透镜阵列耦合,形成一个深紫外光刻的1:1成象的多孔径微小光刻系统.从几何光学理论出发,分析了这个光刻系统综合成象的特点,讨论了这种光刻系统的光学性能,并给出了实验测量方法.     

共焦扫描显微成象系统的点扩散函数再商讨

针对共焦扫描显微成象中物体相对于被扫描点的位置矢量是反向移动的特性,探讨了物体移动后的物函数在此基础上,根据成象的理论进行了计算,得到新的系统点扩散函数表达式,最后根据计算结果分析成象的性质,得出了系统成象的相干性.     

高阶PMD统计特性研究

讨论了三阶偏振模色散(PMD)的统计特性及其对于高阶PMD补偿系统的影响.数值模拟结果表明,随着即时差分群时延(DGD)的增加,二阶PMD单位矢量旋转速率的统计覆盖范围的变化趋势与主偏振态(PSP)旋转斜率的相应变化正好相反.此外,随着即时DGD的增加,二阶PMD模值的统计覆盖范围不断增加,使得高速光通信系统中高阶PMD补偿的实现将更加困难.     

Photolyses of mammalian carboxy-hemoglobin studied by photoacoustic calorimetry

The enthalpy and conformational volume changes in the photolyses of carboxy-hemoglobin (HbCO) of human, bovine, pig, horse and rabbit are investigated by photoacoustic calorimetry. Considering the time scales of the exciting laser pulse and the receiving ultrasound transducers (PVDF films and PZT ceramics), as well as the reaction lifetimes in the photolysis processes of HbCO, the measured results are related to the geminate recombination and tertiary relaxation in photolyses of HbCO. Moreover, the quantum yields of the five mammals are also measured by laser pump-probe technique. The results show that the dynamic parameters, such as enthalpy and conformational volume changes, differ between the processes of the geminate recombination and tertiary relaxation. Also, the dynamic parameters differ among the five mammals although some of them may be consistent with each other.     

荧光层析法研究由胶束溶液分离出的两种抗菌素

喹诺酮药物是一类重要的化学合成抗菌素 ,其临床应用正在不断扩大[1] 。氧氟沙星 (OFLX)和环丙沙星 (CPLX)结构非常相似。本文采用胶束薄层色谱法分离了两种药物。加入 1%氨水的 0 0 0 15mol·L-1十六烷基三甲基溴化铵 (CTAB)和 1%十二烷基磺酸钠 (SDS)的等体积水溶液作为展开剂 ,聚酰胺薄膜作为固定相。考查了CTAB浓度 ,SDS浓度及氨水对氧氟沙星和环丙沙星Rf 值的影响。在选定条件下 ,Rf 值分别为 0 87和 0 76 ,在激发波长为 2 80nm处对其进行了荧光测定。测定了该方法的稳定性和灵敏度。本文首次使用胶束溶液分离了氧氟沙星和环丙沙星     

含Eu3+离子的Na2O-K2 O-SiO2-Al2O3系统玻璃的制备及其变温发射光谱与结构特性

通过高温熔融法制备了含Eu^3 离子的Na2O-K2O-SiO2-Al2O3系统玻璃。在488nm波长光的激发下，系统地研究了上述玻璃从77K到700K温度范围的变温发光特征。发现总的荧光强度首先随温度的升高而大幅度升高，然后随温度的升高而减少，用声子辅助吸收与热激活过程及温度淬灭效应定性地解释了上述强度的变化，同时测量与分析了Eu^3 离子的晶格场参量。结果表明，Eu^3 离子与氧离子的距离随着温度升高而变短，而Eu^3 离子的配位数不随温度而变化。     

温度对应变式位移传感系统灵敏系数的影响

应变式位移传感系统的工作原理是基于电阻应变效应,该系统具有精度高﹑稳定性好等优点,但温度尤其是极端低温环境将影响该系统的灵敏系数,进而影响其测量精度.本文介绍了一种可用于低温环境的应变式位移传感系统,理论分析和实验研究了温度对该系统灵敏系数的影响,研究结果为其在低温环境中的应用提供了一定基础。