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31.
Separation of negatively charged molecules, such as plasmid DNA (pDNA), RNA and endotoxin forms a bottleneck for the development of pDNA vaccine production process. The use of affinity interactions of transition metal ions with these molecules may provide an ideal separation methodology. In this study, the binding behaviour of pDNA, RNA and endotoxin to transition metal ions, either in immobilised or free form, was investigated. Transition metal ions: Cu2+, Ni2+, Zn2+, Co2+ and Fe3+, typically employed in the immobilised metal affinity chromatography (IMAC), showed very different binding behaviour depending on the type of metal ions and their existing state, i.e. immobilised or free. In the alkaline cell lysate, pDNA showed no binding to any of the IMAC chemistries tested whereas RNA interacted significantly with Cu2+-iminodiacetic acid (IDA) and Ni2+-IDA but showed no substantial binding to the rest of the IMAC chemistries. pDNA and RNA, however, interacted to varying degrees with free metal ions in the solution. The greatest selectivity in terms of pDNA and RNA separation was achieved with Zn2+ which enabled almost full precipitation of RNA while keeping pDNA soluble. For both immobilised and free metal ions, ionic strength of solution affected the metal ion-nucleic acid interaction significantly. Endotoxin, being more flexible, was able to interact better with the immobilised metal ions than the nucleic acids and showed binding to all the IMAC chemistries. The specific interactions of immobilised and/or free metal ions with pDNA, RNA and endotoxin showed a good potential, by selectively removing RNA and endotoxin at high efficiency, to develop a simplified pDNA purification process with improved process economics. 相似文献
32.
This paper applies the numerical simulation techniques based on the generalized conservation of circulation (GCC) method to investigate the effects of momentum injection by a leading-edge moving surface on flow past a two-dimensional aerofoil at a Reynolds number of 1000. The stream function and vorticity contours obtained together with the animated flow visualization show that the stall flow region is highly unsteady and consist mainly of large vortices being shed alternately. They are confined to a narrow region near the upper surface of aerofoil asC u (the ratio of the speed of the moving surface to the free stream velocity) is raised. The proximity of vortices to the upper surface of aerofoil at highC u is caused by the ability of free stream to negotiate around the leading edge since the leading-edge moving surface suppresses the growth of boundary layer by reducing the relative between the inviscid flow and the wall. As well-formed large scale vortices are associated with low pressure regions, their proximity to the aerofoil leads to increase in lift as speed ratio increases 相似文献
33.
Hemalatha Murugaiah Chow Lun Teh Kai Chew Loh Ahmad Ramli Mohamad Yahya Nur Asshifa Md Noh Noor Hana Hanif Abu Bakar Daruliza Kernain Rokiah Hashim Yazmin Bustami 《Molecules (Basel, Switzerland)》2021,26(21)
Here, we report the extracellular biosynthesis of silver nanoparticles (AgNPs) and determination of their antibacterial and anticancer properties. We also explore the efficacy of bioAgNPs incorporated in cellulose nanocrystals (CNCs) and alginate (Alg) for the formation of an antibacterial hydrogel film. Streptomyces sp. PBD-311B was used for the biosynthesis of AgNPs. The synthesized bioAgNPs were characterized using UV-Vis spectroscopy, TEM, XRD, and FTIR analysis. Then, the bioAgNPs’ antibacterial and anticancer properties were determined using TEMA and cytotoxicity analysis. To form the antibacterial hydrogel film, bioAgNPs were mixed with a CNC and Alg solution and further characterized using FTIR analysis and a disc diffusion test. The average size of the synthesized bioAgNPs is around 69 ± 2 nm with a spherical shape. XRD analysis confirmed the formation of silver nanocrystals. FTIR analysis showed the presence of protein capping at the bioAgNP surface and could be attributed to the extracellular protein binding to bioAgNPs. The MIC value of bioAgNPs against P. aeruginosa USM-AR2 and MRSA was 6.25 mg/mL and 3.13 mg/mL, respectively. In addition, the bioAgNPs displayed cytotoxicity effects against cancer cells (DBTRG-0.5MG and MCF-7) and showed minimal effects against normal cells (SVG-p12 and MCF-10A), conferring selective toxicity. Interestingly, the bioAgNPs still exhibited inhibition activity when incorporated into CNC/Alg, which implies that the hydrogel film has antibacterial properties. It was also found that bioAgNP-CNC/Alg displayed a minimal or slow release of bioAgNPs owing to the intermolecular interaction and the hydrogel’s properties. Overall, bioAgNP-CNC/Alg is a promising antibacterial hydrogel film that showed inhibition against the pathogenic bacteria P. aeruginosa and MRSA and its application can be further evaluated for the inhibition of cancer cells. It showed benefits for surgical resection of a tumor to avoid post-operative wound infection and tumor recurrence at the surgical site. 相似文献
34.
Leong M. Gan Chwee H. Chew Stig E. Friberg 《Journal of polymer science. Part A, Polymer chemistry》1983,21(2):513-523
The solubility of styrene, its oligomers, and different molecular weight polymers was determined in W/O microemulsions stabilized by sodium dodecyl sulfate and pentanol. The results showed a complex pattern due to the interactions with pentanol, surfactant, and associated structures. 相似文献
35.
A unified boundary integral equation (BIE) is developed for the scattering of elastic and acoustic waves. Traditionally, the elastic and acoustic wave problems are solved separately with different BIEs. The elastic wave case is represented in a vector BIE with the traction and displacement vectors as unknowns whereas the acoustic wave case is governed by a scalar BIE with velocity potential or pressure as unknowns. Although these two waves can be unified in the form of a partial differential equation, the unified form in its BIE counterpart has not been reported. In this work, we derive the unified BIE for these two waves and then show that the acoustic wave case can be derived from this BIE by introducing a shielding loss for small shear modulus approximation; hence only one code needs to be maintained for both elastic and acoustic wave scattering. We also derive the asymptotic Green's tensor for zero shear modulus and solve the corresponding vector equation. We employ the method of moments, which has been widely used in electromagnetics, as a numerical tool to solve the BIEs involved. Our numerical experiments show that it can also be used robustly in elastodynamics and acoustics. 相似文献
36.
We present the general method of constructing curved traversable wormholes in (3+1)-d spacetime and proceed to thoroughly discuss the physics of a zero tidal force metric without cross-terms. The (3+1)-d solution is compared with the recently studied lower-dimensional counterpart, where we identify that the much richer physics—involving pressures and shear forces of the mass-energy fluid supporting the former—is attributed to the mixing of all three spatial coordinates. Our (3+1)-d universe is the lowest dimension where such nontrivial terms appear. An explicit example, the static zero tidal force (3+1)-d catenary wormhole is analysed and we show the existence of a geodesic through it supported locally by non-exotic matter, similar to the (2+1)-d version. A key difference is that positive mass-energy is used to support the entire (3+1)-d catenary wormhole, though violation of the null energy condition in certain regions is inevitable. This general approach of first constructing the geometry of the spacetime and then using the field equations to determine the physics to support it has the potential to discover new solutions in general relativity or to generalise existing ones. For instance, the metric of a time-evolving inflationary wormhole with a conformal factor can actually be geometrically constructed using our method. 相似文献
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39.
Rosenfeld (1971) proved that the Total Colouring Conjecture holds for balanced complete r-partite graphs. Bermond (1974) determined the exact total chromatic number of every balanced complete r-partite graph. Rosenfeld's result had been generalized recently to complete r-partite graphs by Yap (1989). The main result of this paper is to prove that the total chromatic number of every complete r-partite graph G of odd order is Δ (G) + 1. This result gives a partial generalization of Bermond's theorem. 相似文献
40.