A Feasible Add-On Upgrade on a Commercial Two-Photon FLIM Microscope for Optimal FLIM-FRET Imaging of CFP-YFP Pairs

Fluorescence lifetime imaging microscopy (FLIM) based on time-correlated single photon counting (TCSPC) is a widely used method for fluorescence resonance energy transfer (FRET). Here we report a feasible add-on approach to upgrade a commercial two-photon FLIM microscope into a single-photon FLIM microscope which provides optimal FLIM-FRET imaging of FRET pairs consisting of cyan fluorescent proteins (CFPs) as the donor and yellow fluorescent proteins (YFPs) as the acceptor. The capability of the upgraded system is evaluated and discussed, and the imaging performance of the system is demonstrated using FLIM-FRET experiments with a representative CFP-YFP FRET pair (mCerulean-mCitrine).     

基于光外差技术的超宽带频率响应测量系统

介绍了一种利用光外差技术测量光电探测器超宽带频率响应的测试方法。将一个可调谐外腔激光器和一个固定波长的分布反馈激光器（DFB-LD’s）产生的激光输入到光电探测器进行混频。通过对可调激光器腔长的控制,可以在光电探测器产生从DC到上百GHz的拍频信号,在无需额外校准光源的情况下就可以进行光电探测器超宽带频率响应特性的测试,这是该方法最突出的优点。实验证明该方法比较准确、简便、易于操作。在实验中,对两个不同的InGaAs p-i—n探测器进行测量,得到器件的3dB带宽分别为14．4GHz和40GHz。该测量方法对同类实验的研究和应用都具有实用意义。     

A novel construction algorithm of the LDPC code for high-speed long-haul optical transmission systems

According to the transmission characteristics of high-speed long-haul optical transmission system, the main construction method of Low-Density Parity-Check (LDPC) code for optical transmission system is presented and a novel construction algorithm of LDPC code is proposed, the theoretical analysis shows that the parity check matrix of the LDPC code constructed by the proposed construction algorithm has no four-girth phenomenon, this is also theoretically proved out. Both the novel LDPC(3969,3720) code with 6.69% redundancy and the novel LDPC(8281,7920) code with 4.56% redundancy for optical transmission systems are constructed by using the presented construction method and proposed construction algorithm. The simulation results show that the net coding gain (NCG) of the two novel LDPC codes at the eighteenth iteration for the BER of 10^?12 are respectively 1.63 dB and 1.49 dB more than that of the RS(255,239) code in ITU-T G.975. Moreover, the decoding of the LDPC code in the hardware can parallel be implemented, so the decoding speed of the two novel LDPC codes are very rapid, the complexities of implementing the two novel LDPC codes, compared with the concatenated codes in ITU-T G.975.1, are relatively lower, furthermore, the hardware overhead and storage space can relatively be saved and the computation complexity can be reduced in implementing the hardware in the future. As a result, the two novel LDPC codes can better be suitable for high-speed long-haul optical transmission systems.     

机器视觉在激光干涉测量d31中的应用

提出一种应用机器视觉技术的激光干涉法, 可以研究压电材料的逆压电系数. 在传统干涉测量法中引入机器视觉实现干涉图像灰度最佳估计, 使用数字图像处理技术, 一方面应用降噪算法有效去除激光干涉图样中的噪声, 从而确定激光干涉场的光强分布, 另一方面将干涉图样的明暗变化进一步转化为可量度的灰度变化以提高测量分辨力. 通过合理设计算法, 该方法中对位移的理论测量分辨力可以提高达一个数量级, 为干涉光波长的1/1024, 因此在逆压电系数d₃₁ 的实验测量中可以有效提高分辨力. 关键词： 机器视觉 逆压电系数 双光束干涉 数字图像处理     

Shaping of few-cycle laser pulses via a subwavelength structure

We theoretically investigate the propagation of few-cycle laser pulses in resonant two-level dense media with a sub- wavelength structure, which is described by the full Maxwell-Bloch equations without the frame of slowly varying envelope and rotating wave approximations. The input pulses can be shaped into shorter ones with a single or less than one optical cycle. The effect of the parameters of the subwavelength structure and laser pulses is studied. Our study shows that the media with a subwavelength structure can significantly shape the few-cycle pulses into a subcycle pulse, even for the case of chirp pulses as input fields. This suggests that such subwavelength structures have potential application in the shaping of few-cycle laser pulses.     

Octagonal dual-concentric-core photonic crystal fiber for C-band dispersion compensation with low confinement loss

A novel kind of octagonal dual-concentric-core photonic crystal fiber （PCF） is calculated with the finite element method （FEM） and proposed for broadband compensation. In order to realize highly negative dispersion, the hole diameter of the second ring is reduced relative to conventional PCFs. Numerical simulation results show that when the diameters of large holes and small holes are 0.77 pm and 0.5 #m, respectively, and the pitch of the adjacent rings is 1.1 μm, the negative dispersion can achieve about 840 ps.nm^-1.km^-1 at 1 550 nm and the dispersion slope can match with single mode fiber （SMF-28） perfectly. This PCF can compensate the positive dispersion and also its slope of about 50 times its length of SMF-28 fiber, which is suitable for C-band optical fiber telecommunication as a dispersion compensation fiber.     

双平行圆柱形MDM纳米棒等离子体波导的传输特性分析

设计了一种由双平行圆柱形纳米棒构成的金属-介质-金属(MDM)型等离子体波导,采用时域有限差分方法(FDTD)分析了波导结构的传输特性。当光波垂直主轴入射时,电磁场被很好地局限在两纳米棒所形成的中间区域以及介质层中,从而在该波导中能够有效地耦合电磁场能量。在工作波长为1 550 nm的情况下,随着内层金属芯半径的增大,有效折射率减小,传播距离增大;而中间介质层厚度增大时,有效折射率增大,传播距离减小。当外层金属壳厚为20 nm时,电场可以很好地被限制在纳米棒的介质层内。上述结果表明:通过调整波导结构的几何参数可以显著提高金属纳米棒的场限制,降低波导本身的损耗, 使波导的有效折射率和传播长度达到最优化。这种等离子体波导能够实现亚波长的光限制,可以应用于光子器件集成和传感器领域。     

不同光谱成分条件下叶用莴苣矿质元素吸收分析

以叶用莴苣为试验材料,应用ICP-AES等技术,研究了不同光谱成分及其组合条件下生菜对矿质元素的吸收特性。结果显示:(1)生菜常量、微量矿质元素含量比约为Ca:Mg:K:Na:P=5.5:2.5:2.3:1.5:1.0,Fe:Mn:Zn:Cu:B=25.9:5.9:2.8:1.1:1.0,且LED及荧光灯处理下的生菜各元素含量均高于自然光,差异显著;(2)生菜在红蓝组合LED光R/B=1:2.75处理下对K,P,Ca,Mg,B元素的吸收量及累积量均达最大,LED及荧光灯红光均可显著促进生菜对Fe和Cu元素的吸收;(3)矿质元素含量较高及干物质积累量较高的处理均为LED灯R/B=1:2.75和B/W=1:1。     

同时测定旱芹抗性淀粉和抗性低聚糖含量

建立了同时测定旱芹中抗性淀粉（Resistant Starch,RS）和抗性低聚糖（Resistant Oligosacch-arides,RO）含量的方法.通过模拟人体胃肠道的生理条件,除去样品中脂类物质、蛋白质和可溶性淀粉后,分别采用紫外分光光度法和酶-重量法测定RS和RO含量.RS含量在0.0160-0.1600mg/mL（r=0.9993）范围内呈良好的线性关系（n=6）,平均回收率为90.7％,RSD=2.0％ （n=9）;RO含量测定平均回收率为87.3％,RSD=2.9％ （n=9）.实验结果表明：该方法线性、精密度、重复性、稳定性和回收率试验均符合方法学验证要求.该方法操作简便,结果准确,重复性好,为全面测定旱芹膳食纤维（DF）中各组分的含量提供了依据,使旱芹DF含量标示更准确.