排序方式: 共有91条查询结果,搜索用时 15 毫秒
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Song Y Zhang W An Y Cui L Yu C Zhu Z Yang CJ 《Chemical communications (Cambridge, England)》2012,48(4):576-578
We have combined an allosteric molecular beacon for target recognition and guanine-rich DNAzyme for signal amplification to develop a new platform for visual detection of nucleic acids with single-base mismatch detection capability. The fully DNA-structured platform can undergo color change in response to target DNA/RNA, which enables sensitive and selective visual detection in biological samples. 相似文献
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Cui L Lin X Lin N Song Y Zhu Z Chen X Yang CJ 《Chemical communications (Cambridge, England)》2012,48(2):194-196
Based on graphene oxide-protected DNA probes, we have developed a cyclic enzymatic amplification method for sensitive miRNA detection in complex biological samples. By using the quenching nature of graphene oxide for multiple fluorophores, this method can distinguish highly similar miRNA sequences and detect them simultaneously. 相似文献
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Jialu Zhang Bingqian Lin Lingling Wu Mengjiao Huang Xingrui Li Huimin Zhang Jia Song Wei Wang Gang Zhao Yanling Song Chaoyong Yang 《Angewandte Chemie (International ed. in English)》2020,59(33):14115-14119
Microfluidic chips with nano‐scale structures have shown great potential, but the fabrication and cost issues restrict their application. Herein, we propose a conceptually new “DNA nanolithography in a microfluidic chip” by using sub‐10 nm three‐dimensional DNA structures (TDNs) as frameworks with a pendant aptamer at the top vertex (ApTDN‐Chip). The nano‐scale framework ensures that the aptamer is in a highly ordered upright orientation, avoiding the undesired orientation or crowding effects caused by conventional microfluidic interface fabrication processes. Compared with a monovalent aptamer modified chip, the capture efficiency of ApTDN‐Chip was enhanced nearly 60 % due to the highly precise dimension and rigid framework of TDNs. In addition, the scaffolds make DNase I more accessible to the aptamer with up to 83 % release efficiency and 91 % cell viability, which is fully compatible with downstream molecular analysis. Overall, this strategy provides a novel perspective on engineering nano‐scaffolds to achieve a more ordered nano‐topography of microfluidic chips. 相似文献
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A novel sulfamethazine molecularly imprinted polymer (MIP)-coated stir bar for sorptive extraction of eight sulfa drugs from biological samples was prepared. The MIP-coating was about 20 μm thickness with the relative standard deviation (RSD) of 6.7% (n = 10). It was characterized by scanning electron microscope, infrared spectrum, thermogravimetric analysis, and solvent-resistant investigation, respectively. The non-imprinted polymer (NIP)-coating was used for comparison. The adsorptive capacity and selectivity of MIP-coating were evaluated in detail. The MIP-coating showed higher adsorption capability and selectivity than the NIP-coating. The saturated adsorption amount of the MIP-coating was 4.6 times over that of the NIP-coating in toluene. Sulfamethazine could be detected after the MIP-coated stir bar sorptive extraction even at a low concentration of 0.2 μg/L. The MIP-coating also exhibited selective adsorption ability to analogues of the template. A method for the determination of eight sulfa drugs in biological samples by MIP coated stir bar sorptive extraction coupled with high performance liquid chromatography (HPLC) was developed. The extraction conditions, including extraction solvent, extraction time, desorption solvent, desorption time and stirring speed, were optimized. The linear ranges were 1.0-100 μg/L and 2.0-100 μg/L for eight sulfonamides, respectively. The detection limits were within the range of 0.20-0.72 μg/L. The method was successfully applied to simultaneous multi-residue analysis of eight sulfonamides in spiked pork, liver and chicken samples with the satisfactory recoveries. 相似文献
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For several double-helical molecules, the dependence of electronic circular dichroism (ECD) spectra on the molecular and electronic structures is investigated on the basis of TDDFT computations with the B3LYP hybrid functional. The calculations of a model molecule reveal that an acute-angled rotational dihedral angle other than an obtuse-angled rotational dihedral angle between two naphthyls implies Davydov splitting of exciton coupling. For the R enantiomers, the acute-angled rotational dihedral angle implies a negative lowest-energy Cotton effect, which is induced by electron density transfer from binaphthyls to phenyethynyls in the S0?→?S4 excitations. Due to the large positive rotational strength of the S0?→?S1 transition, the lowest-energy Cotton effect of the simplest cyclophane R-1 is positive, where the electron density transfer from phenyethynyls to binaphthyls plays an important role. 相似文献
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Frontispiece: Translating Molecular Recognition into a Pressure Signal to enable Rapid,Sensitive, and Portable Biomedical Analysis
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