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151.
You-Rim Cha Saba Sajjad Kyung Ho Han Tae-Sung Yoon Heung-Seon Shin Sunghyun Kang 《Electrophoresis》2021,42(14-15):1516-1520
Efficient antibody incubation is a vital step for successful western blot. During the incubation, a thin antibody-depleted layer is created around the blotting membrane, which limits antibody binding. Although the conventional batch shaking method is ineffective against it, this layer can be easily disrupted by cyclic draining and replenishing (CDR) of the antibody solution during membrane incubation. Previously, we introduced a closed and rotating cylindrical chamber as a tool to implement CDR for western blots (rCDR). A new open bucket-style chamber was devised for easier operation and the possibility of process automation. Instead of rotation as in rCDR, rocking it back and forth achieved the CDR antibody incubation (R-CDR). The chamber was then equipped with a spreader-rod to facilitate the uniform movement of the antibody solution across the membrane surface. Hence, it was named spreader CDR (S-CDR). Compared to the batch incubation method, both the S-CDR and R-CDR devices produced significantly enhanced signals and developed faster results. There were several additional benefits of using the spreader-rod, which included uniform antibody binding across the membrane, reduced usage of antibodies, and the ability to recover results even from mishandled, creased membranes. The S-CDR device ensures better blots and can be easily implemented in existing western blot protocols. 相似文献
152.
An algorithm, termed the variable grid method (VGM), has been developed to provide enhanced reconstruction by interferometric tomography under the ill-posed problems of restricted angular scanning and incomplete projection that frequently arise in flow measurements. The algorithm is based on variable grid decomposition of a field. The performances of the two versions of the VGM are compared to investigate the effects of the redundancy criteria in generating variable grid elements. Performance comparison is also made with a conventional fixed grid method. The testing is conducted through computer simulation of experiments and reconstruction with a known three-dimensional field that has been produced by computational fluid dynamics. The reconstruction through computer simulation demonstrates the superiority of the two VGMs to the conventional method. Both the maximum and average reconstruction errors are reduced appreciably. The VGM version with stricter parameter contro performs best. The technique can reflect spatial information contained in measured data. Thus, improvement is noticeable in the regions with dense scanning of probing rays. These regions are usually of interest in engineering applications. 相似文献
153.
The present paper proposes a modification of a simple optoelectronic architecture (A. Bergeron et al.: Appl. Opt. 33 (1994) 1463) for carrying out optical thresholding operations. The threshold operation is achieved by means of a feedback loop. The setup is modified by inserting an attenuator adapted in each iteration to the total incident energy measured by an intensity detector. The proposed architecture does not need an additional light source, assures translation invariance and does not break the beam propagation path. The adaptive attenuator permits working under different lighting conditions (illumination, partially occluded objects, etc.). This kind of architecture is suitable for an optical pattern recognition task, optical neural network or optical associative memory. Application of the modified thresholder to the recognition task based on an optical correlator is reported. 相似文献
154.
An empirical formulation is presented to predict the stereochemistry of major osmylation products of allylic alcohols and their derivatives. 相似文献
155.
156.
Youngjoon Cha Haseo Ki Young-One Kim 《Journal of Mathematical Analysis and Applications》2004,290(2):534-541
A sufficient condition for entire functions f and g to be such that the series ∑m=0∞f(m)(0)g(m)/m! represents an entire function is established; and in that case, the growth of the resulting function is described. 相似文献
157.
半合成青霉素侧链合成的研究:Ⅹ.DL—5—(4—羟基苯基)海因的合成 总被引:2,自引:0,他引:2
以乙醛酸、尿素、苯酚为主要原料,在少量醇的存在下,于强酸性介质中经缩合反应合成了DL-5-(4-羟基苯基)海因。采用正交设计试验,找出了影响收率的主要因素,确定了最佳反应条件。醇的加入使产物收率提高到78.7%。 相似文献
158.
Inside Back Cover: An Expanded Porphycene with High NIR Absorptivity That Stabilizes Two Different Kinds of Metal Complexes (Angew. Chem. Int. Ed. 10/2018)
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159.
160.
Tweezer-type and non-tweezer-type ionophores containing dithiocarbamoyl groups on a 7-deoxycholic amide or cholane derivatives were designed and synthesized. Potentiometric evaluation of the poly(vinyl chloride) (PVC) membranes containing those deoxycholic amides/cholanes linked with tweezer-type dithiocarbamoyl moieties showed excellent affinity and selectivity to silver(I) ion over alkali, alkaline earth and other transition metal cations. On the other hand, deoxycholic amides/cholanes substituted with one dithiocarbamoyl group, i.e., non-tweezer-type ionophores, resulted in relatively poor potentiometric sensitivity and detection limits. The enhanced potentiometric properties of newly synthesized tweezer-type dithiocarbamoyl containing ionophores have been further improved by employing silver ion complexing reagent in the internal reference solution, which resulted in greatly reduced detection limit (∼100 ppt) for the electrodes based on them. 相似文献