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51.
We report on the realization and characterization of electro-responsive and pressure sensitive polydimethylsiloxane (PDMS) conductive photonic structures combined with the reconfigurable properties of short pitch cholesteric liquid crystals (aligned in Grandjean configuration). By combining ion-implantation process and surface chemistry functionalization, we have overcome the insulating properties of PDMS and induced long range organization of cholesteric liquid crystals, thus controlling both diffraction and selective Bragg reflection of light by means of external perturbations (electric field, pressure). We have characterized our devices in terms of morphological, optical and electro-optical properties. 相似文献
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Sara Salucci Michela Battistelli Sabrina Burattini Cesare Squillace Barbara Canonico Pietro Gobbi Stefano Papa Elisabetta Falcieri 《Micron (Oxford, England : 1993)》2010,41(8):966-973
Apoptosis is a form of cell death crucial for normal development and tissue homeostasis. Its typical features include chromatin changes, nuclear breakdown, plasma membrane blebbing and splitting of cellular content into apoptotic bodies, that progressively undergo phagocytosis.Apoptosis is considered essential for skeletal muscle development, where defective cells are deleted during differentiation. In addition, it plays a relevant role in several muscle myopathies, as well as in denervation and disuse.The aim of this study was to evaluate muscle cell sensitivity to different apoptotic triggers, acting through different mechanisms of action. Chemical agents, active against distinct intracellular targets, such as mitochondrial respiratory chain and DNA, have been chosen to better highlight cell death mechanisms. To induce apoptosis, C2C12 myoblasts have been exposed to H2O2, staurosporine, cisplatin and etoposide, at different doses and incubation times, and they have been analysed by flow cytometry, scanning and transmission electron microscopy.Flow cytometry analysis revealed a certain subdiploid peak after all treatments. The best apoptotic effect was observable, as confirmed at reverted microscope, at minimum doses and after the major exposure time.At ultrastructural level programmed cell death has been observed. Characteristic chromatin condensation and margination, as well as apoptotic bodies, frequently appeared, even if in the presence of secondary necrosis; surface blebs were also observed during scanning microscopic observation.In particular, exposure to H2O2 or staurosporine showed the largest number of myoblasts in late apoptotic stages and in secondary necrosis. Cisplatin treatments revealed few early apoptotic cells. The analysis of etoposide-induced apoptosis was in agreement with data obtained from flow cytometry, indicating a significant increase of apoptotic cell number.These results suggest that all conditions are able to induce apoptosis in C2C12 myoblasts, which occurs, considering trigger mechanisms of action, mostly following the mitochondrial pathway, if not excluding that due to DNA damage. Therefore, mitochondria permeability alteration is an important step in skeletal muscle programmed cell death. This last conclusion seems to have a significant relevance in understanding the mechanisms involved in muscle disorders, denervation and chronic muscle disuse, conditions frequently characterized by a decline in mitochondrial content and by an increase of mitochondrial apoptosis susceptibility. 相似文献
54.
Patrick Azavant Albert Lichanot Michel Rerat Cesare Pisani 《International journal of quantum chemistry》1996,58(4):419-429
Ab initio calculations of dynamic structure factors are performed with a methodology that requires the crystalline density matrix determined by the periodic Hartree-Fock CRYSTAL code. Amplitudes and orientations of the atomic vibrations are fixed by the mean-square displacements available in the literature. This method, which is here upgraded so as to satisfy normalization conditions, gives rise, for any temperature T, to a “dynamic” density matrix different from the static one. An application to the calculation of the dynamic structure factors of magnesium difluoride (MgF2), which has anisotropic mean-square displacement tensors, is proposed and compared with experiments at 298 K. © 1996 John Wiley & Sons, Inc. 相似文献
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D. Schürmann A. Di Leva L. Gialanella D. Rogalla F. Strieder N. De Cesare A. D'Onofrio G. Imbriani R. Kunz C. Lubritto A. Ordine V. Roca C. Rolfs M. Romano F. Schümann F. Terrasi H. -P. Trautvetter 《The European Physical Journal A - Hadrons and Nuclei》2005,26(2):301-305
The total cross-section of 12C(α,γ)16O was measured for the first time by a direct and ungated detection of the 16O recoils. This measurement in inverse kinematics using the recoil mass separator ERNA in combination with a windowless He
gas target allowed to collect data with high precision in the energy range E = 1.9 to 4.9 MeV. The data represent new information for the determination of the astrophysical S(E) factor. 相似文献
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An analysis of the electronic structure in beryllium slabs with an ab initio LCAO HF method is presented. Band structure, electronic density and density of states were determined for thin films of 1 to 4 atomic layers of Be. A comparison of the electronic structure in films of different thickness with the situation found for bulk Be and Be clusters shows strong modifications in the surface region and a progressive reconstruction of the bulk bonds. 相似文献
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The spectral density method in classical statistical mechanics is formulated and application to a linear classical spin system is made in the simplest approximation. 相似文献
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