Iron(II) phthalocyanine (FePc) over carbon support for oxygen reduction reaction electrocatalysts operating in alkaline electrolyte

Journal of Solid State Electrochemistry - In this work, we initially report a detailed advancement in the utilization of metal-N4 chelate macrocycles in the oxygen reduction reaction (ORR). Then,...     

Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

(1) Background: Nicotine is implicated in the SARS-COV-2 infection through activation of the α7-nAChR and over-expression of ACE2. Our objective was to clarify the role of nicotine in SARS-CoV-2 infection exploring its molecular and cellular activity. (2) Methods: HBEpC or si-mRNA-α7-HBEpC were treated for 1 h, 48 h or continuously with 10⁻⁷ M nicotine, a concentration mimicking human exposure to a cigarette. Cell viability and proliferation were evaluated by trypan blue dye exclusion and cell counting, migration by cell migration assay, senescence by SA-β-Gal activity, and anchorage-independent growth by cloning in soft agar. Expression of Ki67, p53/phospho-p53, VEGF, EGFR/pEGFR, phospho-p38, intracellular Ca²⁺, ATP and EMT were evaluated by ELISA and/or Western blotting. (3) Results: nicotine induced through α7-nAChR (i) increase in cell viability, (ii) cell proliferation, (iii) Ki67 over-expression, (iv) phospho-p38 up-regulation, (v) EGFR/pEGFR over-expression, (vi) increase in basal Ca²⁺ concentration, (vii) reduction of ATP production, (viii) decreased level of p53/phospho-p53, (ix) delayed senescence, (x) VEGF increase, (xi) EMT and consequent (xii) enhanced migration, and (xiii) ability to grow independently of the substrate. (4) Conclusions: Based on our results and on evidence showing that nicotine potentiates viral infection, it is likely that nicotine is involved in SARS-CoV-2 infection and severity.     

Diphenylalanine Motif Drives Self-Assembling in Hybrid PNA-Peptide Conjugates

Peptides and nucleic acids can self-assemble to give supramolecular structures that find application in different fields, ranging from the delivery of drugs to the obtainment of materials endowed with optical properties. Forces that stabilize the “suprastructures” typically are hydrogen bonds or aromatic interactions; in case of nucleic acids, Watson-Crick pairing drives self-assembly while, in case of peptides, backbone hydrogen bonds and interactions between aromatic side chains trigger the formation of structures, such as nanotubes or ribbons. Molecules containing both aromatic peptides and nucleic acids could in principle exploit different forces to self-assemble. In this work we meant to investigate the self-assembly of mixed systems, with the aim to understand which forces play a major role and determine formation/structure of aggregates. We therefore synthesized conjugates of the peptide FF to the peptide nucleic acid dimer “gc” and characterized their aggregates by different spectroscopic techniques, including NMR, CD and fluorescence.     

Towards ultra-responsive biodegradable polysaccharide humidity sensors

Sodium alginate is a biodegradable natural polymer that is derived from algae and is water soluble. Upon immersion in a CaCl₂ solution, a sodium alginate water solution is cross-linked to form water-insoluble calcium alginate. When the sodium alginate water solution is immersed in the CaCl₂ bath via a syringe pump, calcium alginate fibers are produced. By changing the CaCl₂ concentration, calcium alginate fibers with different degrees of cross-linking can be produced. Such fibers were found to differ in mechanical and morphological properties, and more interestingly, were found to possess humidity sensing and conductive properties. Interestingly, the higher the CaCl₂ concentration, the lower the degree of cross-linking, which produced softer fibers with better humidity sensing and conductive properties. The fibers were able to trap water in their structures, and a higher water content increased the conductivity due to the presence of an electrolyte salt in the fiber and due to the polyelectrolyte nature of the fiber itself. The cross-linking and percent shrinking degree, morphology and mechanical properties of the fibers were found to create significant changes in the conductivity and humidity sensing properties of the fibers. High humidity environments led to an increase in the conductivity of the fibers, whereas dry environments led to a decrease in the conductivity. The fibers, especially those with the highest CaCl₂ concentration, were determined to be ultra-responsive to humidity changes and exhibited very good repetition in humidity cycles. These tailored fibers are proposed as novel biodegradable conductive materials for various humidity sensing, robotic and bio-robotic applications.     

Optimal investment in markets with over and under-reaction to information

In this paper we introduce a jump-diffusion model of shot-noise type for stock prices, taking into account over and under-reaction of the market to incoming news. We work in a partial information setting, by supposing that standard investors do not have access to the market direction, the drift, (modeled via a random variable) after a jump. We focus on the expected (logarithmic) utility maximization problem by providing the optimal investment strategy in explicit form, both under full (i.e., from the insider point of view, aware of the right kind of market reaction at any time) and under partial information (i.e., from the standard investor viewpoint, who needs to infer the kind of market reaction from data). We test our results on market data relative to Enron and Ahold. The three main contributions of this paper are: the introduction of a new market model dealing with over and under-reaction to news, the explicit computation of the optimal filter dynamics using an original approach combining enlargement of filtrations with Innovation Theory and the application of the optimal portfolio allocation rule to market data.     

Local search algorithms for finding the Hamiltonian completion number of line graphs

Given a graph G=(V,E), the Hamiltonian completion number of G, HCN(G), is the minimum number of edges to be added to G to make it Hamiltonian. This problem is known to be -hard even when G is a line graph. In this paper, local search algorithms for finding HCN(G) when G is a line graph are proposed. The adopted approach is mainly based on finding a set of edge-disjoint trails that dominates all the edges of the root graph of G. Extensive computational experiments conducted on a wide set of instances allow to point out the behavior of the proposed algorithms with respect to both the quality of the solutions and the computation time.     

Effects of mutational (Lys to Ala) surface charge changes on the redox properties of electrode-immobilized cytochrome c

Untrimethylated yeast iso-1-cytochrome c (cytc) and its single and multiple Lys to Ala variants at the surface lysines 72, 73, and 79 were adsorbed on carboxyalkanethiol self-assembled monolayers (SAMs) on gold, and the thermodynamics and kinetics of the heterogeneous protein-electrode electron-transfer (ET) reaction were determined by voltammetry. The reaction thermodynamics were also measured for the same species freely diffusing in solution. The selected lysine residues surround the heme group and contribute to the positively charged domain of cytc involved in the binding to redox partners and to carboxyl-terminated SAM-coated surfaces. The E degrees' (standard reduction potential) values for the proteins immobilized on SAMs made of 11-mercapto-1-undecanoic acid and 11-mercapto-1-undecanol on gold were found to be lower than those for the corresponding diffusing species owing to the stabilization of the ferric state by the negatively charged SAM. For the immobilized proteins, Lys to Ala substitution(s) do not affect the surface coverage, but induce significant changes in the E degrees' values, which do not simply follow the Coulomb law. The results suggest that the species-dependent orientation of the protein (and thereby of the heme group) toward the negatively charged SAM influences the electrostatic interaction and the resulting E degree' change. Moreover, these charge suppressions moderately affect the kinetics of the heterogeneous ET acting on the reorganization energy and the donor-acceptor distance. The kinetic data suggest that none of the studied lysines belong to the interfacial ET pathway.     

Optical properties of emeraldine salt polymers from ab initio calculations: comparison with recent experimental data

We present the absorption coefficient alpha(omega), the transverse dielectric function epsilon(omega), the optical conductivity sigma(omega), and the reflectance R(omega) calculated for an emeraldine salt conducting polymer in its crystalline 3D polaronic structure. We utilize Kohn-Sham density functional theory (DFT) electronic wavefunctions and energies implemented in the expression of the macroscopic transverse dielectric function in the framework of the band theory without the electron-hole interaction. Contributions of intra-band transitions are taken into account by adding a Drude-like term to the dielectric function calculated ab initio. Comparison with optical properties, recently measured on high-quality emeraldine salts (Lee, K.; Cho, S.; Park, S. H.; Heeger, A. J.; Lee, C.-W.; Lee, S. H. Nature 2006, 441, 65), and with optical absorption spectra, recorded on other emeraldine salts, is very satisfactory. The calculated spectra are discussed in terms of energy-band structure, density of states, inter- and intra-band transitions, and transverse dielectric function.