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21.
Ovine milk proteins were analyzed both by coupling HPLC and electrospray ionization mass spectrometry (ESI-MS) and by flow injection analysis and ESI-MS detection after separation and collection of fractions from gel permeation chromatography. These methods resolved the four ovine caseins and whey proteins and made it possible to study the complexity of these proteins associated with genetic polymorphism, post-translational changes (phosphorylation and glycosylation) and the presence of multiple forms of proteins. The experimental molecular masses of ewe milk proteins were: 19 373 for κ-casein 3P; 25 616 for s2-casein 10P; 23 411 for s1-casein C-8P; 23 750 for β-casein 5P; 18 170 and 18 148 for β-lactoglobulins A and B; 14 152 for -lactalbumin A and 66 322 for serum albumin. 相似文献
22.
Jean-Pierre Morizur Pierre-Francois Casals Jacqueline Ferard ReGine Ropert Maurice Keravec 《Journal of mass spectrometry : JMS》1976,11(9):935-949
The mass spectra of thirty δ-diketones are reported. Their main pathways have been rationalized with the aid of deuterium labelling, metastable ions and high resolution mass measurements. Special attention has been given to ions resulting from the expulsion of neutral molecules such as water preceded, or followed by the loss of ethylene or alkenes. The expulsion may involve an intermediate cyclobutanol ion which would be analogous to the photochemical reaction observed by Yang. 相似文献
23.
Daniel V. Schiavone Diana M. Kapkayeva Qilan Li Molly E. Woodson Andreu Gazquez Casals Prof. Lynda A. Morrison Prof. John E. Tavis Prof. Ryan P. Murelli 《Chemistry (Weinheim an der Bergstrasse, Germany)》2022,28(10):e202104112
Polyoxygenated tropolones possess a broad range of biological activity, and as a result are promising lead structures or fragments for drug development. However, structure–function studies and subsequent optimization have been challenging, in part due to the limited number of readily available tropolones and the obstacles to their synthesis. Oxidopyrylium [5+2] cycloaddition can effectively generate a diverse array of seven-membered ring carbocycles, and as a result can provide a highly general strategy for tropolone synthesis. Here, we describe the use of 3-hydroxy-4-pyrone-based oxidopyrylium cycloaddition chemistry in the synthesis of functionalized 3,7-dimethoxytropolones, 3,7-dihydroxytropolones, and isomeric 3-hydroxy-7-methoxytropolones through complementary benzyl alcohol-incorporating procedures. The antiviral activity of these molecules against herpes simplex virus-1 and hepatitis B virus is also described, highlighting the value of this approach and providing new structure–function insights relevant to their antiviral activity. 相似文献
24.
25.
Hojjat Madadi Mahdi Mohammadi Jasmina Casals‐Terré Roberto Castilla López 《Electrophoresis》2013,34(22-23):3126-3132
PDMS is one of the most common materials used for the flow delivery in the microfluidics chips, since it is clear, inert, nontoxic, and nonflammable. Its inexpensiveness, straightforward fabrication, and biological compatibility have made it a favorite material in the exploratory stages of the bio‐microfluidic devices. If small footprint assays want to be performed while keeping the throughput, high pressure‐rated channels should be used, but PDMS flexibility causes an important issue since it can generate a large variation of microchannel geometry. In this work, a novel fabrication technique based on the prevention of PDMS deformation is developed. A photo‐sensible thiolene resin (Norland Optical Adhesive 63, NOA 63) is used to create a rigid coating layer over the stiff PDMS micropillar array, which significantly reduces the pressure‐induced shape changes. This method uses the exact same soft lithography manufacturing equipment. The verification of the presented technique was investigated experimentally and numerically and the manufactured samples showed a deformation 70% lower than PDMS conventional samples. 相似文献
26.
A survey is reported on the use of additives in organic elemental analysis of compounds containing functional groups that
may compromise the combustion process. 28 substances containing the following functional groups were selected: organic fluorine,
hexafluorophosphate, tetrafluoroborate, tetraphenylborate, sulfonate, phosphine, nitrile, carbide, organometallic. Six additives
(powdered silver, silver tungstate with magnesium oxide, silica oxide, tungsten (VI) oxide with magnesium oxide, powdered
tin, cerium (IV) oxide) in various sample:additive ratios were assayed. Silver tungstate with magnesium oxide (mixture 1:1,
w/w) turned out to be most efficient for the analysis of nitrogen, carbon, hydrogen and sulfur for almost all the compounds
assayed.
Author for correspondence.
Received August 2, 2002; accepted December 12, 2002
Published online May 19, 2003 相似文献
27.
The preparation of a series of complexes formed by 1-methyl-4-mercaptopiperidine (AH) and divalent zinc, cadmium and mercury halides is reported together with some spectral and physical properties. The results of a crystallographic study allows to establish the structure of those of formula [M2(AH)2X4]H2O (M = Zn, Cd, Hg; X = Br, I) consisting of dimers and involving tetrahedral environment with sulphur-bridges for the metal atoms. Polymeric structures are proposed for the complexes of formulae Cd(AH)Cl2 and Hg2Cl4(AH). 相似文献
28.
María Rodríguez-García Guillermo Fernndez-Varo Susana Hidalgo Gabriela Rodríguez Irene Martínez Muling Zeng Eudald Casals Manuel Morales-Ruiz Gregori Casals 《Molecules (Basel, Switzerland)》2022,27(6)
Circulating levels of 2-hydroxybutyrate (2HB) are highly related to glycemic status in different metabolomic studies. According to recent evidence, 2HB is an early biomarker of the future development of dysglycemia and type 2 diabetes mellitus and may be causally related to the progression of normal subjects to impaired fasting glucose or insulin resistance. In the present study, we developed and validated a simple, specific and sensitive gas chromatography-mass spectrometry (GC-MS) method specifically intended to quantify serum levels of 2HB. Liquid–liquid extraction with ethyl acetate was followed by 2 min of microwave-assisted derivatization. The method presented acceptable accuracy, precision and recovery, and the limit of quantification was 5 µM. Levels of 2HB were found to be stable in serum after three freeze-thaw cycles, and at ambient temperature and at a temperature of 4 °C for up to 24 h. Extracts derivatized under microwave irradiation were stable for up to 96 h. No differences were found in 2HB concentrations measured in serum or plasma EDTA samples. In summary, the method is useful for a rapid, precise and accurate quantification of 2HB in serum samples assessed for the evaluation of dysglycemia and diabetes mellitus. 相似文献
29.
Improved high performance liquid chromatographic determination of ginsenosides in Panax ginseng-based pharmaceuticals using a diol column 总被引:3,自引:0,他引:3
A reversed-phase high-performance liquid chromatographic assay for the simultaneous quantitative determination of seven ginsenosides, Rb(1), Rb(2), Rc, Rd, Rg(1), Re and Rf in pharmaceutical preparations is described. Chromatographic separation was achieved in less than 20 min using a 250 x 4 mm Lichrospher, 5 microm, 100 A diol column with detection at 203 nm. The method was validated over the range of 2.5-20 ng/microL using a 20 microL sample volume. The average accuracy at five concentrations was 90-100%, and the within-day and between-day precision ranged from 1 to 7% expressed as coefficient of variation. The detection limit and the quantitation limit of the method were 20 and 50 ng injected for each ginsenoside, respectively. 相似文献
30.
Determination of fat, protein, casein, total solids, and somatic cell count in goat's milk by near-infrared reflectance spectroscopy 总被引:1,自引:0,他引:1
Albanell E Caja G Such X Rovai M Salama AA Casals R 《Journal of AOAC International》2003,86(4):746-752
Analysis using near-infrared reflectance spectroscopy (NIRS) was investigated as a means of predicting quality parameters of goat's milk. Calibration equations were developed with samples from individual goats of Malague?a and Murciano-Granadina dairy breeds at different stages of lactation to obtain a wide range of variation in milk composition. Prediction equations for milk fat, protein, casein, total solids, and somatic cell count (SCC) were developed with 2 sample presentations, liquid drawer (homogenized milk) and aluminium cup (unhomogenized milk), to measure absorbance values, and accuracy of measurements was compared. The multiple correlation coefficient (R) values for homogenized milk were 0.98, 0.96, 0.91, 0.94, and 0.79 and for unhomogenized milk were 0.98, 0.95, 0.92, 0.95, and 0.74 for fat, protein, casein, total solid, and SCC, respectively. To validate the calibrations, an independent set of samples was used. The best simple correlation coefficient (r) values were 0.97, 0.95, 0.91, 0.93, and 0.72 for homogenized milk and 0.97, 0.95, 0.92, 0.95, and 0.79 for unhomogenized milk. The study showed that NIRS is a useful technique for the prediction of fat, protein, casein, total solids, and SCC in unhomogenized goat's milk. 相似文献