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Functionnalized E,Z dienes (?) 2 have been prepared in a highly stereo- and enantioselective manner using chiral pentadienyl iron tricarbonyl complexes 1. 相似文献
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The segregation behaviour of silicon during oxidation of a high-silicon steel has been investigated by AES. The results show that silicon seems to have two states of oxidation: one leading to the formation of SiOx and iron oxides when the oxidation and the following heat treatments in vacuum are performed below 500°C and the other occurring at temperatures higher than 500°C, leading to the formation of SiO2 and segregation of this species toward the surface without oxidation of iron. 相似文献
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The ortho-alkylation of phenols or aniline by catalytic C-H activation and multifunctional catalysis is described. 相似文献
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Morin B Bourhis JM Belle V Woudstra M Carrière F Guigliarelli B Fournel A Longhi S 《The journal of physical chemistry. B》2006,110(41):20596-20608
We used site-directed spin-labeling electron paramagnetic resonance (EPR) spectroscopy to study the induced folding of the intrinsically disordered C-terminal domain of measles virus nucleoprotein (N(TAIL)). Four single-site N(TAIL) mutants (S407C, S488C, L496C, and V517C), located in three conserved regions, were prepared and labeled with a nitroxide paramagnetic probe. We could monitor the gain of rigidity that N(TAIL) undergoes in the presence of either the secondary structure stabilizer 2,2,2-trifluoroethanol (TFE) or one of its physiological partners, namely, the C-terminal domain (XD) of the viral phosphoprotein. The mobility of the spin label grafted at positions 488, 496, and 517 was significantly reduced upon addition of XD, contrary to that of the spin label bound to position 407, which was unaffected. Furthermore, the EPR spectra of spin-labeled S488C and L496C bound to XD in the presence of 30% sucrose are indicative of the formation of an alpha-helix in the proximity of the spin labels. Such an alpha-helix had been already identified by previous biochemical and structural studies. Using TFE we unveiled a previously undetected structural propensity within the N-terminal region of N(TAIL) and showed that its C-terminal region "resists" gaining structure even at high TFE concentrations. Finally, we for the first time showed the reversibility of the induced folding process that N(TAIL) undergoes in the presence of XD. These results highlight the suitability of site-directed spin-labeling EPR spectroscopy to identify protein regions involved in binding and folding events, while providing insights at the residue level. 相似文献
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Direct Identification of Protein–Protein Interactions by Single‐Molecule Force Spectroscopy 下载免费PDF全文
Dr. Andrés M. Vera Dr. Mariano Carrión‐Vázquez 《Angewandte Chemie (International ed. in English)》2016,55(45):13970-13973
Single‐molecule force spectroscopy based on atomic force microscopy (AFM‐SMFS) has allowed the measurement of the intermolecular forces involved in protein‐protein interactions at the molecular level. While intramolecular interactions are routinely identified directly by the use of polyprotein fingerprinting, there is a lack of a general method to directly identify single‐molecule intermolecular unbinding events. Here, we have developed an internally controlled strategy to measure protein–protein interactions by AFM‐SMFS that allows the direct identification of dissociation force peaks while ensuring single‐molecule conditions. Single‐molecule identification is assured by polyprotein fingerprinting while the intermolecular interaction is reported by a characteristic increase in contour length released after bond rupture. The latter is due to the exposure to force of a third protein that covalently connects the interacting pair. We demonstrate this strategy with a cohesin–dockerin interaction. 相似文献
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Pier Giovanni Baraldi Francesca Fruttarolo Mojgan Aghazadeh Tabrizi Romeo Romagnoli Delia Preti Ennio Ongini Hussein El‐Kashef María Dora Carrión Pier Andrea Borea 《Journal of heterocyclic chemistry》2007,44(2):355-361
The reaction between 3‐(dimethylamino)/3,3‐bis(methylthio)‐1‐(substituted)prop‐2‐en‐1‐ones and 4‐substituted‐5‐amino‐1H‐pyrazoles afforded new pyrazole[1,5‐a]pyrimidines structurally related to Zaleplon. The chemical modifications introduced at the 3‐, 5‐, and 7‐positions of the bicyclic structure revealed new promising candidates for the treatment of sleep disorders. 相似文献