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61.
We present noise measurements on YBCO thin films in different conditions of magnetic field and driving current. Noise spectra for non-driven and driven cases (in the flux-creep region) evidence deep differences in vortex dynamics between these two regimes. For the driven case, the effect of applying magnetic field is a reduction in noise, which can be explained by the increase in the fraction of vortices that undergo flux-flow. For the non-driven case, magnetic field has no significative influence on noise, probably due to the absence of Lorentz force that causes coherent movement of vortices. For all magnetic fields studied in this work (0-154 mT) the effect of increasing current is an increase of noise, which is in contrast to the results from other authors. This behavior can be explained by an increase of current induced vortex-antivortex annihilation events. We propose that driven noise has a non-monotonic behavior due to the competition between annihilation events and driving force which causes opposite effects on noise.  相似文献   
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Previous work has reported non-adultlike distortion product otoacoustic emission (DPOAE) suppression in human newborns at f2=6000 Hz, indicating an immaturity in peripheral auditory function. In this study, DPOAE suppression tuning curves (STCs) were recorded as a measure of cochlear function and acoustic admittance/reflectance (YR) in the ear canal recorded as a measure of middle-ear function, in the same 20 infants at birth and through 6 months of age. DPOAE STCs changed little from birth through 6 months, showing excessively narrow and sharp tuning throughout the test period. In contrast, several middle-ear indices at corresponding frequencies shifted systematically with increasing age, although they also remained non-adultlike at 6 months. Linear correlations were conducted between YR and DPOAE suppression features. Only two correlations out of 76 were significant, and all but three YR variables accounted for <10% of the variance in DPOAE suppression tuning. The strongest correlation was noted between admittance phase at 5700 Hz and STC tip-to-tail (R=0.49). The association between middle-ear variables and DPOAE suppression may be stronger during other developmental time periods. Study of older infants and children is needed to fully define postnatal immaturity of human peripheral auditory function.  相似文献   
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Fluorescence, circular dichroism and molecular mechanics have been used to study the complexation of 2,3-dimethyl naphthalenedicarboxylate with 2-hydroxypropyl-α-, -β and -γ-cyclodextrins (HPCDs) in aqueous solution. Emission spectra upon excitation of the naphthalenedicarboxylate group show two bands whose intensity ratio R is quite sensitive to polarity. From the change of R and lifetimes averages <τ> with HPCD concentration and temperature were obtained the stoichiometry, the association constants and the enthalpy and entropy changes during the complexation. R, <τ> and the fluorescence anisotropies (r) extrapolated at [HPCD]→∞ allows us to estimate the polarity and microviscosity of the media surrounding the guest when complexed. In addition, the analysis of quenching and induced circular dichroism experiments and molecular mechanics calculations in the presence of water, provide information about the forces responsible for the complexation and the geometry of the complexes.  相似文献   
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Several Angustifolia Kunth bamboo fibers, which have been previously treated with an alkaline solution, were coated with magnetite particles. The coating of the fibers was achieved by an in-situ co-precipitation method with Fe2+ and Fe3+in NaOH or NH4OH. The fibers were evaluated by chemical analysis using atomic absorption (A.A.) technique, structural characterization by X-ray diffraction (XRD), thermal stability with thermo-gravimetric analysis (TGA) in nitrogen at temperature range between 23 °C and 800 °C and magnetic behavior using vibrating sample magnetometry (VSM) applying a magnetic field between −27 KOe and 27 KOe at room temperature. We found that the thermal stability and magnetization depend of the synthesis method used to cover the Angustifolia Kunth bamboo fibers. In addition, an improved magnetic response was observed when NaOH solution is used to generate the magnetite coating on the fiber surface.  相似文献   
67.
This report describes a method to conjugate lucifer yellow to the external surface of liposomes. The heterobifunctional cross-linking reagentN-succinimidyl 3-(2-pyridyldithio)propionate has been used to activate DMPE molecules. The DMPE-dithiopyridine product has been mixed with DMPC to prepare liposome vesicles. These have been reduced by DTT and finally reacted with lucifer yellow-iodoacetamide to produce the fluorescence-labeled vesicles. The quenching of their fluorescence intensity by Kl is consistent with fully exposed fluorophores. The decay of the fluorescence intensity of the lipid-bound lucifer yellow is biexponential (1=7.9 ns; 2=1.1 ns), with a relative yield of 0.16. When the fluorescent liposomes are mixed with cells, the lucifer yellow-DMPE derivative is transferred. Boar spermatozoa and peripheral human blood lymphocytes have been used as cellular models. The extent of incorporation is dependent on the incubation time and temperature. At 36°C, lucifer yellow fluorescence appears in the spermatozoa cells after 10 min of incubation and reaches its maximum at about 60 min. The fluorescent phospholipid derivative seems to incorporate specifically into membrane structures. The highest labeling ratio is observed with integer, scarcely motile, spermatozoa. A poorer labeling yield (15%) is found with lymphocytes. Interestingly, photobleaching due to epiillumination of the labeled cells is apparently negligible and cells are clearly visible after irradiation times ranging from several minutes to few hours.A preliminary account of this work was presented at the Quarto Simposio su Biotecnologie Biochimiche, Capri, 28–30 June 1992.  相似文献   
68.
Xylanases (EC3.2.1.8) catalyze the hydrolysis of xylan, the major constituent of hemicellulose. The use of these enzymes could greatly improve the overall economics of processing lignocellulosic materials for the generation of liquid fuels and chemicals. The hyperthermophilic archaeon Pyrodictium abyssi, which was originally isolated from marine hot abyssal sites, grows optimally at 97°C and is a prospective source of highly thermostable xylanase. Its endoxylanase was shown to be highly thermostable (over 100 m in at 105°C) and active even at 110°C. The growth of the deep-sea archaeon P. abyssi was investigated using different culture techniques. Among the carbohydrates used, beech wood xylan, birch wood glucuronoxylan and the arabinoxylan from oats pelt appeared to be good inducers for endoxylanase and β-xylosidase production. The highest production of arabinofuranosidase, however, was detected in the cell extracts after growth on xylose and pyruvate, indicating that the intermediate of the tricarboxylic acid cycle acted as a nonrepressing carbon source for the production of thi enzyme. Electron microscopic studies did not show a significant difference in the cell surface (e.g., xylanosomes) when P. abyssi cells were grown on different carbohydrates. The main kinetic parameters of the organism have been determined. The cell yield was shown to be very low owing to incomplete substrate utilization, but a very high maximal specific growth rate was determined (μmax=0.0195) at 90°C and pH 6.0. We also give information on the problems that arise during the fermentation of this hyperthermophilic archaeon at elevated temperatures.  相似文献   
69.
Given a set of markets and a set of products to be purchased on those markets, the Biobjective Traveling Purchaser Problem (2TPP) consists in determining a route through a subset of markets to collect all products, minimizing the travel distance and the purchasing cost simultaneously. As its single objective version, the 2TPP is an NP-hard Combinatorial Optimization problem. Only one exact algorithm exists that can solve instances up to 100 markets and 200 products and one heuristic approach that can solve instances up to 500 markets and 200 products. Since the Transgenetic Algorithms (TAs) approach has shown to be very effective for the single objective version of the investigated problem, this paper examines the application of these algorithms to the 2TPP. TAs are evolutionary algorithms based on the endosymbiotic evolution and other interactions of the intracellular flow interactions. This paper has three main purposes: the first is the investigation of the viability of Multiobjective TAs to deal with the 2TPP, the second is to determine which characteristics are important for the hybridization between TAs and multiobjective evolutionary frameworks and the last is to compare the ability of multiobjective algorithms based only on Pareto dominance with those based on both decomposition and Pareto dominance to deal with the 2TPP. Two novel Transgenetic Multiobjective Algorithms are proposed. One is derived from the NSGA-II framework, named NSTA, and the other is derived from the MOEA/D framework, named MOTA/D. To analyze the performance of the proposed algorithms, they are compared with their classical counterparts. It is also the first time that NSGA-II and MOEA/D are applied to solve the 2TPP. The methods are validated in 365 uncapacitated instances of the TPPLib benchmark. The results demonstrate the superiority of MOTA/D and encourage further researches in the hybridization of Transgenetic Algorithms and Multiobjective Evolutionary Algorithms specially the ones based on decomposition.  相似文献   
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