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121.
We discuss several examples of synchronous dynamical phenomena in
coupled cell networks
that are unexpected from symmetry considerations, but are natural
using a theory developed by Stewart, Golubitsky, and Pivato. In particular
we demonstrate patterns of synchrony in networks with small numbers of cells
and in lattices (and periodic arrays) of cells that cannot readily be
explained by conventional symmetry considerations. We also show that
different types of dynamics can coexist robustly in single solutions of
systems of coupled identical cells. The examples include a
three-cell system exhibiting equilibria, periodic, and quasiperiodic states
in different cells; periodic $2n\times 2n$ arrays of cells that generate
$2^n$ different patterns of synchrony from one symmetry-generated solution;
and systems exhibiting multirhythms (periodic solutions with rationally
related periods in different cells). Our theoretical results include the
observation that reduced equations on a center manifold of a skew product
system inherit a skew product form. 相似文献
122.
Phase diversity is a phase-retrieval algorithm that uses a pair of intensity images taken symmetrically about the wave front to be determined. If these images are taken about the system input pupil this is equivalent to a curvature-sensing algorithm. Traditionally a defocus aberration kernel is used to produce the phase-diverse data. We present a generalization of this method to allow the use of other functions as the diversity kernel. We discuss the necessary and sufficient conditions that such a function must satisfy for use in a null wave-front sensor. Computer simulations were used to validate these results. 相似文献
123.
Aradhana Pangasa Aaron R. Jex Matthew J. Nolan Bronwyn E. Campbell Shane R. Haydon Melita A. Stevens Robin B. Gasser 《Electrophoresis》2010,31(10):1637-1647
The high‐resolution analysis of genetic variation has major implications for the identification of parasites and micro‐organisms to species and subspecies as well as for population genetic and epidemiological studies. In this study, we critically assessed the effectiveness of a PCR‐based restriction endonuclease fingerprinting (REF) method for the detection of mutations in the 60 kDa glycoprotein gene (gp60) of Cryptosporidium, a genus of parasitic protists of major human and animal health importance globally. This gene displays substantial intraspecific variability in sequence, particularly in a TCA (perfect and imperfect) microsatellite region, is present as a single copy in the nuclear genome and is used widely as a marker in molecular epidemiological studies of Cryptosporidium hominis and C. parvum, the two predominant species that infect humans. The results of this study demonstrated an exquisite capacity of REF to detect nucleotide variability in the gp60 gene within each of the two species. The differentiation of genotypes/subgenotypes based on REF analysis was supported by targeted sequencing, allowing the detection of levels of variation as low as a single‐nucleotide transversion for amplicons of ∼1 kb in size. The high‐throughput potential and relatively low‐cost of REF make it a particularly useful tool for large‐scale genetic analyses of C. hominis and C. parvum. REF could also be utilized for comparative surveys of genetic variability across large nuclear genomic regions. Such analyses of Cryptosporidium in clinical and environmental samples by REF have important implications for identifying sources of infection, modes of transmission and/or possible infectivity to humans, thus assisting in the surveillance and control of cryptosporidiosis. Given its excellent mutation detection capacity, REF should find broad applicability to various single‐copy genes as well as a wide range of other protozoan and metazoan parasites. (The nucleotide sequences reported in this article are available in the GenBank database under accession numbers GU214343–GU214371). 相似文献
124.
This paper describes simple methods of demonstrating macroscale spontaneous assembly. These demonstrations can illustrate topics in college or high school chemistry courses, such as the thermodynamics of crystallization and the chelate effect. The assembling units are built from a combination of magnetic and conventional LEGO building blocks. Unlike many other spontaneous-assembly experiments, these assemblies do not require surface flotation to form. 相似文献
125.
126.
G R Campbell G W Odling-Smee B J Rowlands G B Irvine 《Biomedical chromatography : BMC》1989,3(2):75-78
A method is described for the separation of unconjugated bile acids and their glycine and taurine conjugates in a single step with adequate sensitivity for analysis of serum samples. Separation was carried out over a period of 80 min using a linear gradient with increasing concentrations of methanol in aqueous ammonium dihydrogen phosphate buffer, on an ODS Spherisorb column. Spectrofluorometric detection of NADH, formed as the column eluate passed through a column of immobilized 3 alpha-hydroxysteroid dehydrogenase, enabled amounts less than 40 pmol to be quantified. The reaction was carried out at neutral pH so that the lifetime of the enzyme column was increased, compared to other methods where a pH nearer 9.0 is commonly used. Flow rates were optimized to give comparable peak area for both primary and secondary bile acids. 相似文献
127.
Ohne Zusammenfassung 相似文献
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