全文获取类型
收费全文 | 68篇 |
免费 | 0篇 |
专业分类
化学 | 19篇 |
晶体学 | 1篇 |
力学 | 2篇 |
数学 | 2篇 |
物理学 | 44篇 |
出版年
2018年 | 1篇 |
2016年 | 1篇 |
2014年 | 1篇 |
2013年 | 5篇 |
2012年 | 4篇 |
2011年 | 1篇 |
2010年 | 3篇 |
2009年 | 2篇 |
2008年 | 1篇 |
2007年 | 3篇 |
2005年 | 1篇 |
2004年 | 6篇 |
2002年 | 3篇 |
2000年 | 1篇 |
1999年 | 6篇 |
1998年 | 2篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1982年 | 1篇 |
1976年 | 3篇 |
1935年 | 2篇 |
排序方式: 共有68条查询结果,搜索用时 78 毫秒
51.
Development of a chip-based capillary gel electrophoresis method for quantification of a half-antibody in immunoglobulin G4 samples 总被引:2,自引:0,他引:2
Vasilyeva E Woodard J Taylor FR Kretschmer M Fajardo H Lyubarskaya Y Kobayashi K Dingley A Mhatre R 《Electrophoresis》2004,25(21-22):3890-3896
A method based on microfluidic technology was developed to support quantitative analysis of recombinant monoclonal immunoglobulin G4 (IgG4) antibody samples. The assay was performed on an Agilent 2100 Bioanalyzer in combination with the Protein 200 Plus LabChip Kit and the Protein 200 Plus assay software. Capillary electrophoresis principles have been transferred to a chip format that integrates all separation, staining, virtual destaining, and detection steps. The method is referred to in this paper as chip-based capillary gel electrophoresis (GelChip-CE method). The GelChip-CE method under nonreducing conditions proved to be a quantitative test for half-antibody determination in IgG4 samples. Similar to the traditional nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method, the GelChip-CE method includes a denaturing step prior to separation. We showed that denaturing the sample by heating resulted in an artificial increase in the amount of half-antibody detected, which could be prevented by addition of N-ethylmaleimide to the sample buffer. The GelChip-CE method allowed for analysis of IgG4 samples with more accuracy, higher precision, and a faster turnaround time than SDS-PAGE and reversed-phase high-performance liquid chromatography (RP-HPLC). 相似文献
52.
53.
54.
We derive the implications for anisotropies in the cosmic microwave background following from a model of inflation in which a bare cosmological constant is gradually screened by an infrared process in quantum gravity. The model predicts that the amplitude of scalar perturbations is AS = (2.0 ± 0.2) · 10—5, that the tensor-to-scalar ratio is r ≈︂ 1.7 · 10—3, and that the scalar and tensor spectral indices are n ≈︂ 0.97 and nT ≈︂ —2.8 · 10—4, respectively. By comparing the model's power spectrum with the COBE 4-year RMS quadrupole, the mass scale of inflation is determined to be M = (0.72 ± 0.03) · 1016 GeV. At this scale the model produces about 108 e-foldings of inflation, so another prediction is Ω = 1. PACS numbers: 04.60.-m, 98.80.Cq 相似文献
55.
R. P. Woodard 《Foundations of Physics》2014,44(2):213-233
I review a class of nonlocally modified gravity models which were proposed to explain the current phase of cosmic acceleration without dark energy. Among the topics considered are deriving causal and conserved field equations, adjusting the model to make it support a given expansion history, why these models do not require an elaborate screening mechanism to evade solar system tests, degrees of freedom and kinetic stability, and the negative verdict of structure formation. Although these simple models are not consistent with data on the growth of cosmic structures many of their features are likely to carry over to more complicated models which are in better agreement with the data. 相似文献
56.
The Johansen–Ledoit–Sornette (JLS) model of rational expectation bubbles with finite-time singular crash hazard rates has been developed to describe the dynamics of financial bubbles and crashes. It has been applied successfully to a large variety of financial bubbles in many different markets. Having been developed over a decade ago, the JLS model has been studied, analyzed, used and criticized by several researchers. Much of this discussion is helpful for advancing the research. However, several serious misconceptions seem to be present within this literature both on theoretical and empirical aspects. Several of these problems stem from the fast evolution of the literature on the JLS model and related works. In the hope of removing possible misunderstanding and of catalyzing useful future developments, we summarize these common questions and criticisms concerning the JLS model and synthesize the current state of the art and existing best practice. 相似文献
57.
58.
J.W. Vanderhoff M.S. El-Aasser F.J. Micale E.D. Sudol CM. Tseng A. Silwanowicz 《Journal of Dispersion Science and Technology》2013,34(3-4):231-246
Monodisperse polystyrene latexes are prepared by seeded emulsion polymerization; however, sizes larger than 2μm are difficult to prepare because of the creaming and settling of the particles, and their sensitivity to mechanical shear. Preparation in space would obviate the creaming and settling, and allow agitation just sufficient for good heat transfer and mixing. Three polymerizations yielding 3-5μm size particles were carried out successfully on the third flight of the “Columbia” launched March 22, 1982; however, four polymerizations yielding sizes up to 10μm on the fourth flight launched June 27, 1982, were incomplete owing to apparatus malfunction. The results of these polymerizations and the prospects of developing a preparative space process are reviewed 相似文献
59.
We study a conjecture involving the invariant volume of the past light-cone from an arbitrary observation point back to a
fixed initial value surface. The conjecture is that a fourth order differential operator which occurs in the theory of conformal
anomalies gives 8π when acted upon the invariant volume of the past light-cone. We show that an extended version of the conjecture is valid
for an arbitrary homogeneous and isotropic geometry. First order perturbation theory about flat spacetime reveals a violation
of the conjecture which, however, vanishes for any vacuum solution of the Einstein equation. These results may be significant
for constructing quantum gravitational observables, for quantifying the back-reaction on spacetime expansion and for alternate
gravity models which feature a timelike vector field. 相似文献
60.