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31.
Site‐Specific Protection and Dual Labeling of Human Epidermal Growth Factor (hEGF) for Targeting,Imaging, and Cargo Delivery 下载免费PDF全文
Dr. Michael H. Sonntag Jenny Ibach Dr. Lidia Nieto Dr. Peter J. Verveer Prof. Dr. Luc Brunsveld 《Chemistry (Weinheim an der Bergstrasse, Germany)》2014,20(20):6019-6026
Well‐defined human epidermal growth factor (hEGF) constructs featuring selectively addressable labels are urgently needed to address outstanding questions regarding hEGF biology. A protein‐engineering approach was developed to provide access to hEGF constructs that carry two cysteine‐based site‐specific orthogonal labeling sites in multi‐milligram quantities. Also, a site‐selective (de)protection and labeling approach was devised, which allows selective modification of these hEGF constructs. The hEGF, featuring three native disulfide bonds, was expressed featuring additional sulfhydryl groups, in the form of cysteine residues, as orthogonal ligation sites at both the N and C termini. Temporary protection of the N‐terminal cysteine unit, in the form of a thiazolidine ring, avoids interference with protein folding and enables sequential labeling in conjunction with the cysteine residue at the C terminus. Based on thus‐generated hEGF constructs, sequential and site‐specific labeling with a variety of molecular probes could be demonstrated, thus leading to a biological fully functional hEGF with specifically incorporated fluorophores or protein cargo and native cellular targeting and uptake profiles. Thus, this novel strategy provides a robust entry to high‐yielding access of hEGF and rapid and easy site‐specific and multifunctional dual labeling of this growth factor. 相似文献
32.
An on-bead cyclization protocol of beta 3-peptides was developed, providing easy access to cyclic beta 3-peptides. With this methodology, a small library of helical cyclic beta 3-peptides was synthesized and investigated with CD spectroscopy. Covalent bridging of two side chains in beta 3-peptides significantly stabilized their helical conformation in aqueous solutions and turned out to be superior to the previously described electrostatic interactions. 相似文献
33.
Dual‐Input Regulation and Positional Control in Hybrid Oligonucleotide/Discotic Supramolecular Wires 下载免费PDF全文
Dr. Miguel Ángel Alemán García Eva Magdalena Estirado Dr. Lech‐Gustav Milroy Prof. Dr. Luc Brunsveld 《Angewandte Chemie (International ed. in English)》2018,57(18):4976-4980
The combination of oligonucleotides and synthetic supramolecular systems allows for novel and long‐needed modes of regulation of the self‐assembly of both molecular elements. Discotic molecules were conjugated with short oligonucleotides and their assembly into responsive supramolecular wires studied. The self‐assembly of the discotic molecules provides additional stability for DNA‐duplex formation owing to a cooperative effect. The appended oligonucleotides allow for positional control of the discotic elements within the supramolecular wire. The programmed assembly of these hybrid architectures can be modulated through the DNA, for example, by changing the number of base pairs or salt concentration, and through the discotic platform by the addition of discotic elements without oligonucleotide handles. These hybrid supramolecular‐DNA structures allow for advanced levels of control over 1D dynamic platforms with responsive regulatory elements at the interface with biological systems. 相似文献
34.
Brunsveld L Meijer EW Prince RB Moore JS 《Journal of the American Chemical Society》2001,123(33):7978-7984
Circular dichroism spectroscopy has been used to study the self-assembly of two series of m-phenylene ethynylene oligomers in highly polar solvents. The helical conformation of shorter oligomer lengths was found to be stabilized in aqueous acetonitrile solutions, while longer oligomers began to interact intermolecularly. The intermolecular aggregation of the oligomers in aqueous solutions revealed a chain length dependent association that required the presence of a stable helical conformation. Evidence for intermolecular interactions is provided by Sergeants and Soldiers experiments in which the twist sense bias of a chiral oligomer is transferred to an achiral oligomer. 相似文献
35.
Dr. Ralph P. G. Bosmans Wouter E. Hendriksen Mark Verheijden Dr. Rienk Eelkema Prof. Pascal Jonkheijm Prof. Jan H. van Esch Prof. Luc Brunsveld 《Chemistry (Weinheim an der Bergstrasse, Germany)》2015,21(50):18466-18473
Protein immobilization on surfaces, and on lipid bilayers specifically, has great potential in biomolecular and biotechnological research. Of current special interest is the immobilization of proteins using supramolecular noncovalent interactions. This allows for a reversible immobilization and obviates the use of harsh ligation conditions that could denature fragile proteins. In the work presented here, reversible supramolecular immobilization of proteins on lipid bilayer surfaces was achieved by using the host–guest interaction of the macrocyclic molecule cucurbit[8]uril. A fluorescent protein was successfully immobilized on the lipid bilayer by making use of the property of cucurbit[8]uril to host together a methylviologen and the indole of a tryptophan positioned on the N‐terminal of the protein. The supramolecular complex was anchored to the bilayer through a cholesterol moiety that was attached to the methylviologen tethered with a small polyethylene glycol spacer. Protein immobilization studies using a quartz crystal microbalance (QCM) showed the assembly of the supramolecular complexes on the bilayer. Specific immobilization through the protein N‐terminus is more efficient than through protein side‐chain events. Reversible surface release of the proteins could be achieved by washing with cucurbit[8]uril or buffer alone. The described system shows the potential of supramolecular assembly of proteins and provides a method for site‐specific protein immobilization under mild conditions in a reversible manner. 相似文献
36.
Yong‐Xiang Chen Dr. Sebastian Koch Dr. Katharina Uhlenbrock Dr. Katrin Weise Dr. Debapratim Das Dr. Lothar Gremer Dr. Luc Brunsveld Prof. Dr. Alfred Wittinghofer Prof. Dr. Roland Winter Prof. Dr. Gemma Triola Dr. Herbert Waldmann Prof. Dr. 《Angewandte Chemie (International ed. in English)》2010,49(35):6090-6095
37.
Marion K. Müller Luc Brunsveld Prof. Dr. 《Angewandte Chemie (International ed. in English)》2009,48(16):2921-2924
Binding bacteria : Discotic molecules self‐assemble into columnar supramolecular polymers that show strong polyvalent binding to bacteria by virtue of mannose ligands attached at their periphery (orange; see picture). The reversible formation of the supramolecular polymers allows simple mixing of differently substituted monomers and the optimization of bacterial aggregation.
38.
Uhlenheuer DA Wasserberg D Haase C Nguyen HD Schenkel JH Huskens J Ravoo BJ Jonkheijm P Brunsveld L 《Chemistry (Weinheim an der Bergstrasse, Germany)》2012,18(22):6788-6794
Supramolecular assembly of proteins on surfaces and vesicles was investigated by site-selective incorporation of a supramolecular guest element on proteins. Fluorescent proteins were site-selectively labeled with bisadamantane by SNAP-tag technology. The assembly of the bisadamantane functionalized SNAP-fusion proteins on cyclodextrin-coated surfaces yielded stable monolayers. The binding of the fusion proteins is specific and occurs with an affinity in the order of 10(6) M(-1) as determined by surface plasmon resonance. Reversible micropatterns of the fusion proteins on micropatterned cyclodextrin surfaces were visualized by using fluorescence microscopy. Furthermore, the guest-functionalized proteins could be assembled out of solution specifically onto the surface of cyclodextrin vesicles. The SNAP-tag labeling of proteins thus allows for assembly of modified proteins through a host-guest interaction on different surfaces. This provides a new strategy in fabricating protein patterns on surfaces and takes advantage of the high labeling efficiency of the SNAP-tag with designed supramolecular elements. 相似文献
39.
Prince RB Brunsveld L Meijer EW Moore JS 《Angewandte Chemie (International ed. in English)》2000,39(1):228-230
Cooperative interactions among the side chains of the helically folded phenylene-ethynylene oligomer shown (n=2, 4, 6, 8, 10, 12, 14, 16, 18) can induce a twist sense bias. Therefore, the side chains can play more than just an ancillary role in these conformationally ordered oligomers. The onset of the twist sense bias lags significantly behind the appearance of helical conformations, possibly because a large ensemble of "collapsed" conformations is initially formed. 相似文献
40.
A series of m-phenylene ethynylene oligomers containing nonpolar, (S)-3,7-dimethyl-1-octanoxy side chains have been synthesized and studied. In apolar alkane solvents, oligomers of sufficient length (n > 10) were found to adopt a helical conformation with a large twist sense bias. In contrast, in chloroform the oligomers adopt a random coil conformation. Surprisingly, the strong twist sense bias was determined to be highly time dependent and is partially attributed to intermolecular aggregation. 相似文献