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排序方式: 共有93条查询结果,搜索用时 15 毫秒
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Yamashita K Ogura D Yamaguchi Y Miyazaki M Briones MP Nakamura H Maeda H 《Analytical and bioanalytical chemistry》2005,382(7):1477-1483
Microfluidic systems enable superior control of fluidics. We have developed a novel size-separation method utilizing secondary flow within a microchannel. Using confocal fluorescence microscopy and computer simulation, we confirmed that separation occurred as a result of specific molecular localization in the curving part of the microchannel. Maximum separation efficiency was achieved by optimizing microchannel design and flow rate for individual separation targets. In addition, more effective separation was achieved by use of plural microchannel curves. This method was used for sequence-selective DNA sensing. Double-stranded DNA formed by hybridization between target DNA and a complementary probe had different elution profiles from those of the single-stranded non-complementary sequence. Moreover, the response depends on the length of the DNA molecules. This method does not require immobilization of either probe or target DNA, because all reactions occurred in the solution phase. Such features may reduce experimental error and the difference between data from different operators. 相似文献
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Belz JE Potterveld DH Anthony P Arnold RG Arrington J Beck D Beise EJ Bosted PE Bulten H Chapman MS Coulter KP Dietrich F Ent R Epstein M Filippone BW Gao H Gearhart RA Geesaman DF Hansen J Holt RJ Jackson HE Jones CE Keppel CE Kinney ER Kuhn S Lee K Lorenzon W Lung A Makins NC Margaziotis DJ McKeown RD Meziani ZE Milner RG Mueller B Napolitano J Nelson J O'Neill TG Papavassiliou V Petratos GG Rock SE Segel RE Spengos M Szalata ZM Tao LH van Bibber K van den Brand JF White JL Zeidman B 《Physical review letters》1995,74(5):646-649
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Lee K Miller MA Smith A Hansen J Bloch C van den Brand JF Bulten HJ Ent R Goodman CD Jacobs WW Jones CE Korsch W Leuschner M Lorenzon W Marchlenski D Meyer HO Milner RG Neal JS Pancella PV Pate SF Pitts WK von Przewoski B Rinckel T Sowinski J Sperisen F Sugarbaker E Tschalär C Unal O Zhou ZL 《Physical review letters》1993,70(6):738-741
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Douradinha B van Dijk M van Gemert GJ Khan SM Janse CJ Waters AP Sauerwein RW Luty AJ Silva-Santos B Mota MM Epiphanio S 《Journal of immune based therapies and vaccines》2011,9(1):6-5
Background
The induction of sterile immunity and long lasting protection against malaria has been effectively achieved by immunization with sporozoites attenuated by gamma-irradiation or through deletion of genes. For mice immunized with radiation attenuated sporozoites (RAS) it has been shown that intrahepatic effector memory CD8+ T cells are critical for protection. Recent studies have shown that immunization with genetically attenuated parasites (GAP) in mice is also conferred by liver effector memory CD8+ T cells.Findings
In this study we analysed effector memory cell responses after immunization of GAP that lack the P52 protein. We demonstrate that immunization with p52 -GAP sporozoites also results in a strong increase of effector memory CD8+ T cells, even 6 months after immunization, whereas no specific CD4+ effector T cells response could be detected. In addition, we show that the increase of effector memory CD8+ T cells is specific for the liver and not for the spleen or lymph nodes.Conclusions
These results indicate that immunization of mice with P. berghei p52 -GAP results in immune responses that are comparable to those induced by RAS or GAP lacking expression of UIS3 or UIS4, with an important role implicated for intrahepatic effector memory CD8+ T cells. The knowledge of the mediators of protective immunity after immunization with different GAP is important for the further development of vaccines consisting of genetically attenuated sporozoites. 相似文献89.
A. Mazuelas L. Tapfer A. Ruiz F. Briones K. Ploog 《Applied Physics A: Materials Science & Processing》1992,55(6):582-585
An ultrathin two monolayers thick layer of GaP sandwiched within a GaAs matrix was grown by atomic layer molecular beam epitaxy (ALMBE). The X-ray interference effect (Pendellösung) was used to determine the structural parameters such as thickness, lattice parameter, chemical composition, and strain. Excellent agreement between the experimental rocking curve and the simulation using the dynamical theory of X-ray diffraction was found indicating the high quality of the sample. Analysis of the scans in symmetrical (004) and asymmetrical (224) reflections, sensitive to both perpendicular and parallel strain, shows that the GaP layer is coherent with the substrate, i.e., it is below the critical thickness in agreement with critical thickness theories. Despite the competition for incorporation between arsenic and phosphorus the experimental GaP thickness is found to be identical to the nominal growth value, demonstrating full incorporation of phosphorus when growing by ALMBE. No significant out-diffusion or segregation of P is observed. 相似文献
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Trischuk W de Troconiz JF Truitt S Tseng J Turini N Ukegawa F Valls J Vejcik S Velev G Vidal R Vilar R Vologouev I Vucinic D Wagner RG Wagner RL Wahl J Wallace NB Walsh AM Wang C Wang CH Wang MJ Watanabe T Waters D Watts T Webb R Wenzel H 《Physical review letters》2000,84(5):835-840
We present the results of a search for pair production of a fourth-generation charge -1 / 3 quark (b(')) in sqrt[s] = 1.8 TeV p&pmacr; collisions using 88 pb(-1) of data obtained with the Collider Detector at Fermilab. We assume that both quarks decay via the flavor-changing neutral current process b(')-->bZ(0) and that the b(') mass is greater than m(Z)+m(b). We studied the decay mode b(')b(');-->Z(0)Z(0)b&bmacr; where one Z0 decays into e(+)e(-) or &mgr;(+)&mgr;(-) and the other decays hadronically, giving a signature of two leptons plus jets. An upper limit on the sigma(p&pmacr;-->b(')b(');)x[B(b(')-->bZ(0))](2) is established as a function of the b(') mass. We exclude at 95% confidence level a b(') quark with mass between 100 and 199 GeV/c(2) for B(b(')-->bZ(0)) = 100%. 相似文献