Spezifische Drehung einiger vegetabilischer Prote?ne

Ohne ZusammenfassungAus einem gütigst zugesandten Abdruck aus dem Journal of the American chemical Society25, No. 8, bearbeitet und übersetzt von Dr. Griessmayer.     

A simple colorimetric FIA method for the determination of pyrite oxidation rates

A method for the rapid determination of the oxidation rate of naturally occurring pyrite (FeS₂) samples is presented. The progress of the oxidation reaction was followed by measurement of the concentration of total dissolved iron using flow injection analysis. Iron was determined using UV-vis detection after reaction with the colorimetric reagent 5-sulfosalicylic acid in the presence of ammonia. The calibration function was linear between 5 and 150 mg L⁻¹, and the detection limit was 0.46 mg L⁻¹. The relative standard deviation was typically less than 1% (n = 10) and the measurement frequency was 60/h. The method was used to quantify the oxidation rate of 10 ground and cleaned pyrite samples (53 μm < x < 106 μm) from various international locations that were subjected to accelerate oxidation in acidic hydrogen peroxide. Results of these experiments showed that there was almost an order of magnitude of difference in oxidation rates of the pyrite samples.     

Design of functional ferritin-like proteins with hydrophobic cavities

Ferritin four-helix bundle subunits self-assemble to create a stable multimer with a large central hydrophilic cavity where metal ions bind. To explore the versatility of this reaction vessel, computational design was used to generate cavities with increasingly apolar surface areas inside a dodecameric ferritin-like protein, Dps. Cavity mutants, in which as many as 120 surface accessible hydrophilic residues were replaced with hydrophobic amino acids, were shown to still assemble properly using size-exclusion chromatography and dynamic light scattering measurements. Wild-type Dps exhibited highly cooperative subunit folding and assembly, which was monitored by changes in Trp fluorescence and UV circular dichroism. The hydrophobic cavity mutants showed distinctly less cooperative unfolding behavior, with one mutant forming a partially assembled intermediate upon guanidine denaturation. Although the stability of Dps to such denaturation decreased with increasing apolar surface area, all proteins exhibited high melting temperatures, T(m) = 74-90 degrees C. Despite the large number of mutations, near-native ability to mineralize iron was maintained. This work illustrates the versatility of the ferritin scaffold for engineering large protein cavities with novel properties.     

General entanglement scaling laws from time evolution

We establish a general scaling law for the entanglement of a large class of ground states and dynamically evolving states of quantum spin chains: we show that the geometric entropy of a distinguished block saturates, and hence follows an entanglement-boundary law. These results apply to any ground state of a gapped model resulting from dynamics generated by a local Hamiltonian, as well as, dually, to states that are generated via a sudden quench of an interaction as recently studied in the case of dynamics of quantum phase transitions. We achieve these results by exploiting ideas from quantum information theory and tools provided by Lieb-Robinson bounds. We also show that there exist noncritical fermionic systems and equivalent spin chains with rapidly decaying interactions violating this entanglement-boundary law. Implications for the classical simulatability are outlined.     

Biocatalytic nylon nanofibrous membranes

Nylon-6 nanofibrous membranes (NFM) have been prepared, characterized and used to build-up electrochemical biosensing devices. The assembly and the functioning of biocatalytic NFM are described in connection with the physical and the covalent immobilization of glucose oxidase for the detection of glucose. Effects of the enzyme loading, the mediator, the pH, the surface acidity and the kinetic of the catalysis have been thoroughly investigated. The results show that NFM allow the binding of proteins without the need for the hydrolysis step, in contrast to the nylon film. Furthermore, the high surface-to-volume ratio of the NFM allow superior loading of the enzyme with respect to thin film technology. The immobilization step does not affect the permeability of the coating to the mediator used. These results give evidence that NFM are a promising and inexpensive coating for a novel electrochemical transducer.