Simultaneous spectrophotometric determination of minoxidil and tretinoin by the H-point standard addition method and partial least squares

A simple, sensitive and selective spectrophotometric method for simultaneous determination of tretinoin and minoxidil using partial least square (PLS) calibration and H-point standard addition method (HPSAM) is described. The results of the H-point standard addition method show that minoxidil and tretinoin can be determined simultaneously with the concentration ratio of tretinoin to minoxidil varying from 2: 1 to 1: 33 in mixed samples. A partial least squares multivariate calibration method for the analysis of binary mixtures of tretinoin and minoxidil was also developed. The total relative standard error for applying the PLS method to eleven synthetic samples in the concentration range of 0–10 μg mL⁻¹ tretinoin and 0–32 μg mL⁻¹ minoxidil was 2.59 %. Both proposed methods (PLS and HPSAM) were also successfully applied in the determination of tretinoin and minoxidil in several synthetic pharmaceutical solutions.     

Thermodynamic denaturation of β-lactoglobulin in the presence of cetylpyridinium chloride

In this work, we determined the stability parameters of bovine β-lactoglobulin, variant A, (BLG-A), in relation to their transition curves induced by cetylpyridinium chloride (CPC) as a cationic surfactant. The experiments took place over the temperature range of 298 K to 358 K. For each transition curve at any specific temperature, the conventional method of analysis, which assumes a linear concentration dependence of the pre- and post-transition base lines, gave the most realistic values for ΔG_D(H₂O). Results show that the minimum value of ΔG_D(H₂O) occurs at T = 328 K. Using the Gibbs–Helmholtz equation, the values of enthalpy, ΔH_D, and entropy, ΔS_D, of denaturation have been calculated considering temperature dependence of ΔG_D at any specified concentration of CPC. The values of 12.05 kJ · mol⁻¹, 18.54 kJ · mol⁻¹, and 18.32 J · mol⁻¹ · K⁻¹, were obtained for ΔG_D(H₂O), ΔH_D(H₂O), and ΔS_D(H₂O), respectively. The results show that the enthalpy term dominates the entropy term.     

Isothermal titration calorimetric study on the interaction of apo-human transferrin with sodium n-dodecyl sulfate

Isothermal titration calorimetry (ITC) was utilized at conditions close to physiologic (50 mM HEPES buffer, pH 7.4 and 160 mM NaCl) and at various temperatures (20, 25, 30, 35, and 40 °C) to evaluate the enthalpy and heat capacity changes for the interactions of sodium n-dodecyl sulfate (SDS) as an anionic surfactant with apo-human transferrin (apo-hTf). The obtained results are very informative due to importance of heat capacity change as a major thermodynamic quantity that is one of the richest potential sources of information in physical terms. The obtained precise curves and heat capacity curves were interpreted in terms of molecular events such as specific and non-specific binding and the unfolding process. A three step mechanism for the interaction of SDS with apo-hTf has been figured out on basis of ITC studies: $ N \leftrightarrow I_{1} \leftrightarrow I_{2} \leftrightarrow D $ , where N, I, and D correspond to native, partially unfolded, and denatured states, respectively.     

Energitics of micellizaion of sodium n-dodecyl sulfate at physiological conditions using isothermal titration calorimetry

The curing kinetics of nanocomposites based on phenolic resol cured with triethylamine (TEA) containing different amounts of organic montmorillonite was analyzed by differential scanning calorimetry. Kissinger-Akahira-Sunose (KAS) model-free kinetics has been applied to correlate the dynamic cure behaviour in the presence of modified montmorillonite. The effect in the curing of the use of different clay modifiers has also been studied. A commercial clay with hydroxyl groups (Cloisite 30B) and a customized montmorillonite (PheMMT) whose reactive groups induce condensation reactions with the resol matrix have been used. Strong dependency of activation energy on apparent conversion has been observed for all compounds.     

Equation of State of Protoneutron Star Matter

We have calculated the equation of state of protoneutron star matter by using the lowest order constrained variational method. In our calculations, the modern Argonne potential (AV₁₈) together with its older model potential (AV₁₄) are used. It is found that the equation of state for high lepton fraction is stiffer than for low lepton fraction. It is seen that the increasing effect of pressure due to high lepton fraction and due to entropy are comparable. It is shown that the temperature and adiabatic index depend on the values of both entropy and lepton fraction.     

Interaction of a homologous series of <Emphasis Type="Italic">n</Emphasis>-alkyl trimethyl ammonium bromides with eggwhite lysozyme

The interaction of a series of n-alkyl trimethyl ammonium bromides (C₁₂, C₁₄ and C₁₆) with egg white lysozyme have been studied using fluorescence and UV-Vis spectroscopies and isothermal titration calorimetry (ITC). The trend of variation of molar absorptivity at 281 nm, quantum yields (λ_ex=281 nm) and heat of interaction with respect to surfactant concentration, were measured. The spectrophotometric results show that the hydrophobic interactions have a major role in denaturation mechanism and it would be increased with increasing in hydrocarbon tail length of surfactant. The ITC results indicated the two-step mechanism for unfolding of lysozyme due to its interaction with surfactants.     

Type-II Intermittency from Markov Binary Block Visibility Graph Perspective

In this work, we study the type-II intermittency based on asymptotic modes and the optimized Markov binary visibility graphs perspective. In fact, we investigate the behavior of a dynamical system in the vicinity of subcritical Hopf bifurcations of pre-fixed point, fixed point, and post-fixed point using networks language. We use self maps in order to generate asymptotic modes in the type-II intermittency. We find their properties based on statistical tools such as the length between reinjection points and the mean length and also length distributions. Numerical results show that asymptotic modes affect on the trajectory and the length between reinjection points of type-II intermittency in situations of pre-fixed point, fixed point, and post-fixed point, however their mean length are approximately similar to each other. For further illustration, we compute the degree distribution of the complex network generated by type-II intermittency. Experimental results are found to agree well with the analytical results derived from the optimized Markov binary visibility graph.