全文获取类型
收费全文 | 182篇 |
免费 | 2篇 |
专业分类
化学 | 162篇 |
力学 | 3篇 |
数学 | 7篇 |
物理学 | 12篇 |
出版年
2023年 | 1篇 |
2022年 | 9篇 |
2021年 | 5篇 |
2020年 | 3篇 |
2019年 | 5篇 |
2018年 | 4篇 |
2017年 | 2篇 |
2016年 | 5篇 |
2015年 | 6篇 |
2014年 | 8篇 |
2013年 | 12篇 |
2012年 | 14篇 |
2011年 | 15篇 |
2010年 | 11篇 |
2009年 | 6篇 |
2008年 | 17篇 |
2007年 | 12篇 |
2006年 | 14篇 |
2005年 | 6篇 |
2004年 | 10篇 |
2003年 | 6篇 |
2002年 | 1篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1998年 | 1篇 |
1995年 | 1篇 |
1992年 | 2篇 |
1982年 | 1篇 |
1977年 | 1篇 |
排序方式: 共有184条查询结果,搜索用时 15 毫秒
21.
Kornelija Lasi Ana Bokuli Astrid Mili Biljana Nigovi Ana Mornar 《Biomedical chromatography : BMC》2019,33(8)
This paper presents lipophilicity and bio‐mimetic property determination of 15 phytoestrogens, namely biochanin A, daidzein, formononetin, genistein, genistein‐4,7‐dimethylether, prunetin, 3,4,7‐trihydroxyisoflavon, 4,6,7‐trihydroxyisoflavon, 4,6,7‐trimethoxyisoflavon, daidzin, genistin, ononin, sissotrin, coumestrol and coumestrol dimethylether. High‐performance liquid chromatography with fast gradient elution and Caco‐2 cell line were used to determine the physicochemical properties of selected phytoestrogens. Lipophilicity was determined on octadecyl‐sylane stationary phase using pH 2.0 and pH 7.4 buffers. Immobilized artificial membrane chromatography was used for prediction of interaction with biological membranes. Protein binding was measured on human serum albumin and α‐1‐acid‐glycoprotein (AGP) stationary phases. Caco‐2 assay was used as a gold standard for assessing in vitro permeability. The obtained results differentiate phytoestrogens according to their structure where aglycones show significantly higher lipophilicity, immobilized artificial membrane partitioning, AGP binding and Caco‐2 permeability compared with glucosides. However, human serum albumin binding was very high for all investigated compounds. Furthermore, a good correlation between experimentally obtained chromatographic parameters and in silico prediction was obtained for lipophilicity and human serum albumin binding, while the somewhat greater difference was obtained for AGP binding and Caco‐2 permeability. 相似文献
22.
Svetlana M. Stanišić Ljubiša M. Ignjatović Dragan D. Manojlović Biljana P. Dojčinović 《Central European Journal of Chemistry》2011,9(3):481-491
This paper presents the extraction of cations from a soil sample, type ranker on serpentinite, in deionized water, by use
of three different extraction techniques. The first extraction technique included the use of a rotary mixer, the second technique
involved the use of a microwave digestion system with different extraction temperatures, and the third technique employed
an ultrasonic bath with different extraction times. Ion chromatography was used for determining the concentration of Li, Na,
K, Ca, Mg and ammonium ions in soil extracts with subsequent determination of concentrations for all cations, except for ammonium
ion extraction, conducted by Inductively Coupled Plasma-Atomic Emission Spectrometry. The results of cation extractions showed
that microwave assisted extraction was most efficient for the Li, Na, K, Ca, Mg, Co, Mn, Ni, Pb and ammonium ions. Use of
a rotary mixer for extraction was most efficient for Cd and Zn ions, while use of ultrasound bath was most efficient for Cr,
Cu, Fe and Al ions. Several times higher amount of cations extracted by the most efficient, compared to the second best technique,
under optimal conditions, were noticed in the case of: Ca, Mg, Co, Mn, Fe, Al, and Zn ions. 相似文献
23.
Adrenocortical carcinoma (ACC) is a rare malignancy with an incompletely understood pathogenesis and a poor prognosis. The adrenalytic activity of mitotane has made it the most important single drug in the treatment of ACC. Unfortunately, the exact mechanism of mitotane action is still unknown. It is believed that mitotane belongs to the class of drugs that require metabolic transformation by cytochrome P450 for therapeutic action; therefore determination of plasma levels of not only mitotane but also its metabolites would help in carrying out the treatment. The objective of this work was to develop and validate an SPE‐HPLC method for simultaneous determination of mitotane and its metabolites in different biological fluids. The sample preparation consisted of a solid‐phase extraction on a Discovery DSC18 cartridge, while analysis of extracts was performed on a Symmetry C18 column. The usefulness of the proposed method was confirmed by analysis of plasma, red cell and urine samples from patient chronically treated with 1.5 g of mitotane. The patient involved in this study had a high plasma concentration of mitotane and none of the investigated metabolites were found. In order to investigate whether the polymorphism of CYP2C9 and CYP2C19 enzymes could be related to the metabolism of mitotane, RT‐PCR analysis was performed. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
24.
Neboja . Panteli Bojan Boi Bojana B. Zmejkovski Neboja R. Banjac Biljana Doj
inovi Ludger A. Wessjohann Goran N. Kaluerovi 《Molecules (Basel, Switzerland)》2021,26(11)
The synthesis of novel triphenyltin(IV) compounds, Ph3SnLn (n = 1–3), with oxaprozin (3-(4,5-diphenyloxazol-2-yl)propanoic acid), HL1, and the new propanoic acid derivatives 3-(4,5-bis(4-methoxylphenyl)oxazol-2-yl)propanoic acid, HL2, and 3-(2,5-dioxo-4,4-diphenylimidazolidin-1-yl)propanoic acid, HL3, has been performed. The ligands represent commercial drugs or their derivatives and the tin complexes have been characterized by standard analytical methods. The in vitro antiproliferative activity of both ligands and organotin(IV) compounds has been evaluated on the following tumour cell lines: human prostate cancer (PC-3), human colorectal adenocarcinoma (HT-29), breast cancer (MCF-7), and hepatocellular cancer (HepG2), as well as on normal mouse embryonic fibroblast cells (NIH3T3) with the aid of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-12 diphenyltetrazolium bromide) and CV (crystal violet) assays. Contrary to the inactive ligand precursors, all organotin(IV) carboxylates showed very good activity with IC50 values ranging from 0.100 to 0.758 µM. According to the CV assay (IC50 = 0.218 ± 0.025 µM), complex Ph3SnL1 demonstrated the highest cytotoxicity against the caspase 3 deficient MCF-7 cell line. Inductively coupled plasma mass spectrometry (ICP-MS) analysis indicated a two-fold lower concentration of tin in MCF-7 cells in comparison to platinum. To investigate the mechanism of action of the compound Ph3SnL1 on MCF-7 cells, morphological, autophagy and cell cycle analysis, as well as the activation of caspase and ROS/RNS and NO production, has been performed. Results suggest that Ph3SnL1 induces caspase-independent apoptosis in MCF-7 cells. 相似文献
25.
Enisa Selimović Tatjana Vulović Biljana Petrović Živadin D. Bugarčić Jovana Bogojeski 《Transition Metal Chemistry》2013,38(6):635-640
The kinetics of the complex formation reactions of two [(TL tBu)PtCl]+ and [Pt(tpdm)Cl]+ complexes (TL tBu = 2,6-bis[(1,3-di-tert-butylimidazolin-2-imino)methyl]pyridine and tpdm = terpyridinedimethane) with N-donor ligands, l-histidine (L-His), inosine (Ino), inosine-5′-monophosphate (5′-IMP) and guanosine-5′-monophosphate (5′-GMP), were studied. All reactions were studied under pseudo-first-order conditions as a function of nucleophile concentration and temperature in aqueous 0.1 M NaClO4 solution in the presence of 10 mM NaCl using variable-temperature Uv–Vis spectrophotometry. The order of reactivity of the studied ligands is L-His > Ino > 5′-GMP > 5′-IMP. This order of reactivity is in relation to their electronic properties and structures. The mechanism of the substitution reactions is associative in nature as supported by the negative entropy of activation. 相似文献
26.
An improved procedure for intramolecular cyclization of tertiary alkenols using benzeneselenyl halides has been developed. We found that cyclization can be facilitated by pyridine. Thus, in the presence of an equimolar amount of pyridine, a chemospecific reaction could be observed that resulted in formation of corresponding cyclic ethers, and quantitative yields were achieved instantaneously under extremely mild experimental conditions. The effect of the halide ion of the selenylating reagent is not significant, both halides generally giving equal results © 2001 John Wiley & Sons, Inc. Heteroatom Chem 12:475–479, 2001 相似文献
27.
Biljana Jančić-Stojanović Igor Popović Anđelija Malenović Darko Ivanović Mirjana Medenica 《Chromatographia》2010,71(9-10):799-804
In this paper optimization of chromatographic retention of ramipril and its five impurities employing factorial design is presented. On the basis of preliminary experiments three factors were chosen as inputs (acetonitrile content, pH of the mobile phase and buffer concentration) and retention factor as output. As optimal full factorial design 23 was chosen, factors were examined at two different levels “low” and “high”. Three replications at zero level were added in order to check linearity and complete statistical tests. Relationship between inputs and output is presented in form of second order interaction model. Adequacy of model was explained using analysis of variance. After analysis of results optimal chromatographic conditions were set. Separations were conducted on a C18 column with a mixture of acetonitrile and water phase (TEA in potassium dihydrogen phosphate) in ratio 23:77 v/v. Finally, the LC method was validated and applied for quality control analysis of commercially available tablets. The proposed method is simpler and faster as compared to existing official methods and therefore more adequate for routine control of ramipril during shelf life. Also a general approach which includes factorial design in method optimization offers a possibility for predicting and following the chromatographic behavior of such complex mixtures. 相似文献
28.
29.
Presented in this paper is a simulation of the interaction of neutrons emitted from a cylindrical Am–Be source with a CR-39 detector. A Fortran90 program, called Neutron.f90, has been written to determine the specifications of secondary particles (alpha particle or proton) and the kinematics of the interactions. A Monte Carlo method was used to simulate which interactions occurred with the constituents of the CR-39 detector, H, C and O atoms. Due to the detector composition, elastic and nonelastic scattering of neutrons is possible. In this paper, nonelastic scattering includes: inelastic scattering; the emission of an alpha particle (n,α) reaction and the emission of a proton (n,p) reaction. Cross sections of other reactions are very small and negligible in comparison with the above mentioned. The energy deposited per unit mass per one neutron was calculated. In addition the contribution to the energy deposited by the alpha particles and protons were determined separately. The program Neutron.f90 computes the coordinates of points in which interactions occur i.e. where secondary particles appear. The energy and angular distributions of protons are also represented in this paper. Another Fortran90 program, called Track_Visibility.f90, was written to calculate the number of visible proton tracks and to calculate the energy deposited per one neutron per one visible track. 相似文献
30.
Cathryn E Langley Biljana Sljuki? Craig E Banks Richard G Compton 《Analytical sciences》2007,23(2):165-170
The modification of carbon powder with manganese dioxide using a wet impregnation procedure with electrochemical characterisation of the modified powder is described. The process involves saturation of the carbon powder with manganese(II) nitrate followed by thermal treatment at ca. 773 K leading to formation of manganese(IV) oxide on the surface of the carbon powder. The construction of composite electrodes based on manganese dioxide modified carbon powder and epoxy resin is also described, including optimisation of the percentage of the modified carbon powder. Composite electrodes showed attractive performances for electroanalytical applications, proving to be suitable for the electrochemical detection of hydrogen peroxide, ascorbic acid and nitrite ions with limits of detection comparable to the detection limits achieved by other analytical techniques. The results obtained for detection of these analytes, together with composite electrodes flexible design and low cost offers potential application of composite electrodes in biosensors. 相似文献