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排序方式: 共有143条查询结果,搜索用时 15 毫秒
41.
42.
Nilson C. Bernardes 《Journal of Mathematical Analysis and Applications》2012,389(1):558-561
We establish some results that complement the work of Castillo and Papini (2007) [4] on distance types. In particular, we give a positive answer to two problems posed by them in the case of Banach spaces with separable duals. 相似文献
43.
Beatriz G. Bernardes Pasquale Del Gaudio Paulo Alves Raquel Costa Carlos A. García-Gonzalz Ana Leite Oliveira 《Molecules (Basel, Switzerland)》2021,26(13)
Wounds affect one’s quality of life and should be managed on a patient-specific approach, based on the particular healing phase and wound condition. During wound healing, exudate is produced as a natural response towards healing. However, excessive production can be detrimental, representing a challenge for wound management. The design and development of new healing devices and therapeutics with improved performance is a constant demand from the healthcare services. Aerogels can combine high porosity and low density with the adequate fluid interaction and drug loading capacity, to establish hemostasis and promote the healing and regeneration of exudative and chronic wounds. Bio-based aerogels, i.e., those produced from natural polymers, are particularly attractive since they encompass their intrinsic chemical properties and the physical features of their nanostructure. In this work, the emerging research on aerogels for wound treatment is reviewed for the first time. The current scenario and the opportunities provided by aerogels in the form of films, membranes and particles are identified to face current unmet demands in fluid managing and wound healing and regeneration. 相似文献
44.
He Li Joo Conde Ana Guerreiro Gonalo J. L. Bernardes 《Angewandte Chemie (International ed. in English)》2020,59(37):16023-16032
The bioorthogonal inverse‐electron‐demand Diels–Alder (IEDDA) cleavage reaction between tetrazine and trans‐cyclooctene (TCO) is a powerful way to control the release of bioactive agents and imaging probes. In this study, a pretargeted activation strategy using single‐walled carbon nanotubes (SWCNTs) that bear tetrazines (TZ@SWCNTs) and a TCO‐caged molecule was used to deliver active effector molecules. To optimize a turn‐on signal by using in vivo fluorescence imaging, we developed a new fluorogenic near‐infrared probe that can be activated by bioorthogonal chemistry and image tumours in mice by caging hemicyanine with TCO (tHCA). With our pretargeting strategy, we have shown selective doxorubicin prodrug activation and instantaneous fluorescence imaging in living cells. By combining a tHCA probe and a pretargeted bioorthogonal approach, real‐time, non‐invasive tumour visualization with a high target‐to‐background ratio was achieved in a xenograft mice tumour model. The combined advantages of enhanced stability, kinetics and biocompatibility, and the superior pharmacokinetics of tetrazine‐functionalised SWCNTs could allow application of targeted bioorthogonal decaging approaches with minimal off‐site activation of fluorophore/drug. 相似文献
45.
Nilson C. Bernardes 《Semigroup Forum》1997,54(1):128-130
46.
A.T. Bernardes D. Stauffer J. Kertész 《The European Physical Journal B - Condensed Matter and Complex Systems》2002,25(1):123-127
The network of Barabasi and Albert, a preferential growth model where a new node is linked to the old ones with a probability
proportional to their connectivity, is applied to Brazilian election results. The application of the Sznajd rule, that only
agreeing pairs of people can convince their neighbours, gives a vote distribution in good agreement with reality
Received 19 September 2001 and Received in final form 2 November 2001 相似文献
47.
A Minimal,Unstrained S‐Allyl Handle for Pre‐Targeting Diels–Alder Bioorthogonal Labeling in Live Cells
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Dr. Bruno L. Oliveira Zijian Guo Dr. Omar Boutureira Ana Guerreiro Dr. Gonzalo Jiménez‐Osés Dr. Gonçalo J. L. Bernardes 《Angewandte Chemie (International ed. in English)》2016,55(47):14683-14687
The unstrained S‐allyl cysteine amino acid was site‐specifically installed on apoptosis protein biomarkers and was further used as a chemical handle and ligation partner for 1,2,4,5‐tetrazines by means of an inverse‐electron‐demand Diels–Alder reaction. We demonstrate the utility of this minimal handle for the efficient labeling of apoptotic cells using a fluorogenic tetrazine dye in a pre‐targeting approach. The small size, easy chemical installation, and selective reactivity of the S‐allyl handle towards tetrazines should be readily extendable to other proteins and biomolecules, which could facilitate their labeling within live cells. 相似文献
48.
Bernardes GJ Chalker JM Errey JC Davis BG 《Journal of the American Chemical Society》2008,130(15):5052-5053
An efficient and robust oxidative elimination of cysteine to dehydroalanine has been discovered. The reaction is induced by O-mesitylenesulfonylhydroxylamine (MSH) and is compatible with methionine. The key elimination has been executed on protein surfaces and allows ready access to different post-translationally modified proteins through conjugate addition of sulfur nucleophiles to dehydroalanine. Treatment of the resulting thioether with MSH results in regeneration of dehydroalanine, allowing a "functional switch" by subsequent addition of a different thiol. 相似文献
49.
Marc Alexandre Duarte Gigonzac Lilian Souza Teodoro Lysa Bernardes Minasi Thaís Cidália Vieira Aparecido Divino da Cruz 《Electrophoresis》2016,37(23-24):3076-3078
Fragile X syndrome (FXS) is the most common cause of inherited intellectual disability. The most common etiology of the syndrome is expansion and methylation of a CGG trinucleotide at chromosome region Xq27.3 involving FMR1 (fragile X mental retardation 1 gene). This disorder is commonly underdiagnosed in children and adolescents, given the high clinical variability. In Brazil, molecular diagnosis of FXS by CE does not exist in the public health system. The current standard for separation and identification of DNA fragment sizes is 50 cm CE, which is uncommon in public genotyping laboratories. This study describes the standardization of 36 cm CE for fragment analysis of samples from patients with intellectual disability suggestive of FXS. Genomic dsDNA was isolated from patients and amplified by PCR using the FMR1 AmplideX® Kit. It was then possible to detect changes in repeat length of FMR1, such as full mutation and premutation. Thus, the proposed standardization proved to be effective for the diagnosis of FXS, permitting suitable genetic counseling for families. Inclusion of molecular testing such as this in the Brazilian public health service bridges the gap between available technologies and effective diagnosis, universalizing access to genetic testing in central Brazil. 相似文献
50.
Biodiesel fuel production by the transesterification reaction of soybean oil using immobilized lipase 总被引:1,自引:0,他引:1
Bernardes OL Bevilaqua JV Leal MC Freire DM Langone MA 《Applied biochemistry and biotechnology》2007,137(1-12):105-114
The enzymatic alcoholysis of soybean oil with methanol and ethanol was investigated using a commercial, immobilized lipase (Lipozyme RMIM). The effect of alcohol (methanol or ethanol), enzyme concentration, molar ratio of alcohol to soybean oil, solvent, and temperature on biodiesel production was determined. The best conditions were obtained in a solvent-free system with ethanol/oil molar ratio of 3.0, temperature of 50 degrees C, and enzyme concentration of 7.0% (w/w). Three-step batch ethanolysis was most effective for the production of biodiesel. Ethyl esters yield was about 60% after 4 h of reaction. 相似文献