Strategies for quantifying C(60) fullerenes in environmental and biological samples and implications for studies in environmental health and ecotoxicology

Fullerenes are sphere-like molecules with unique physico-chemical properties, which render them of particular interest in biomedical research, consumer products and industrial applications. Human and environmental exposure to fullerenes is not a new phenomenon, due to a long history of hydrocarbon-combustion sources, and will only increase in the future, as incorporation of fullerenes into consumer products becomes more widespread for use as anti-aging, anti-bacterial or anti-apoptotic agents.An essential step in the determination of biological effects of fullerenes (and their surface-functionalized derivatives) is establishment of exposure-assessment techniques. However, in ecotoxicological studies, quantification of fullerenes is performed infrequently because robust, uniformly applicable analytical approaches have yet to be identified, due to the wide variety of sample types. Moreover, the unique physico-chemistry of fullerenes in aqueous matrices requires reassessment of conventional analytical approaches, especially in more complex biological matrices (e.g., urine, blood, plasma, milk, and tissue).Here, we present a review of current analytical approaches for the quantification of fullerenes and propose a consensus approach for determination of these nanomaterials in a variety of environmental and biological matrices.     

SPECTRAL CHARACTERIZATION OF LIGHT-REDUCIBLE CYTOCHROME IN A PLASMA MEMBRANE-ENRICHED FRACTION AND IN OTHER MEMBRANES FROM CAULIFLOWER INFLORESCENCES

Abstract— Low temperature spectroscopy has been used to characterize microsomal fractions obtained from cauliflower inflorescences ( Brasska oleracea L.) by differential centrifugation and partition in an aqueous polymer two-phase system. The plasma membrane-enriched fraction (U₃) was found to contain one dominant b -cytochrome, which could be reduced both by blue light and by dithionite. An action spectrum of the blue light-induced absorbance change [LIAC, Δ(A₄₃₀—A₄₁₀)] associated with the reversible reduction of this b -type cytochrome indicated that the primary light-receptor was a flavin-like compound. Another microsomal fraction (L₃) containing membranes from mitochondria, endoplasmic reticulum and other organelles also contained light-reducible cytochrome. One of these could be identified as cytochrome c oxidase, and another may be identical to cytochrome b ₅ of the endoplasmic reticulum.     

Kovasznay Mode Decomposition of Velocity-Temperature Correlation in Canonical Shock-Turbulence Interaction

The correlation coefficient R_uT between the streamwise velocity and temperature is investigated for the case of canonical shock-turbulence interaction, motivated by the fact that this correlation is an important component in compressible turbulence models. The variation of R_uT with the Mach number, the turbulent Mach number, and the Reynolds number is predicted using linear inviscid theory and compared to data from DNS. The contributions from the individual Kovasznay modes are quantified. At low Mach numbers, the peak post-shock R_uT is determined by the acoustic mode, which is correctly predicted by the linear theory. At high Mach numbers, it is determined primarily by the vorticity and entropy modes, which are strongly affected by nonlinear and viscous effects, and thus less well predicted by the linear theory.     

Fast neighborhood search for two- and three-dimensional nesting problems

In this paper we present a new heuristic solution method for two-dimensional nesting problems. It is based on a simple local search scheme in which the neighborhood is any horizontal or vertical translation of a given polygon from its current position. To escape local minima we apply the meta-heuristic method Guided Local Search.     

Viscoelastic characteristics of UV polymerized poly(ethylene glycol) diacrylate networks with varying extents of crosslinking

The viscoelastic relaxation characteristics of ultraviolet crosslinked networks based on poly(ethylene glycol) diacrylate [PEGDA] have been investigated by dynamic mechanical methods. Effective crosslink density in the networks was varied via the use of PEGDA prepolymers of different molecular weight, or by the introduction of controlled amounts of water in the reaction mixture. In all cases examined, fully amorphous networks were obtained. Time–temperature superposition was applied to obtain master curves of storage modulus versus frequency across the glass transition, and these could be satisfactorily described using the Kohlrausch–Williams–Watts relaxation function. The glass transition temperature (T_g), relaxation breadth, and fragility of the segmental relaxation were correlated with the effective crosslink density obtained in the networks. Gas permeation measurements on the PEGDA/water networks indicated only a very modest variation in gas transport properties, despite the sizeable variation in apparent crosslink density achieved in these materials. This result suggests that the controlling structural factor for gas transport in the networks is not simply crosslink density, and that attempts to correlate gas transport to network structure must necessarily consider the broader relationships between crosslink density, segmental mobility, and fractional free volume. © 2006 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 44: 2058–2070, 2006     

Induced Ramsey-type theorems

We present a unified approach to proving Ramsey-type theorems for graphs with a forbidden induced subgraph which can be used to extend and improve the earlier results of Rödl, Erd?s-Hajnal, Prömel-Rödl, Nikiforov, Chung-Graham, and ?uczak-Rödl. The proofs are based on a simple lemma (generalizing one by Graham, Rödl, and Ruciński) that can be used as a replacement for Szemerédi's regularity lemma, thereby giving much better bounds. The same approach can be also used to show that pseudo-random graphs have strong induced Ramsey properties. This leads to explicit constructions for upper bounds on various induced Ramsey numbers.