Simple method for the determination of rosiglitazone in human plasma using a commercially available internal standard

To the best of our knowledge, bioanalytical methods to determine rosiglitazone in human plasma reported in literature use internal standards that are not commercially available. Our purpose was to develop a simple method for the determination of rosiglitazone in plasma employing a commercially available internal standard (IS). After the addition of celecoxib (IS), plasma (0.25 mL) samples were extracted into ethyl acetate. The residue after evaporation of the organic layer was dissolved in 750 microL of mobile phase and 50 microL was injected on to HPLC. The separation was achieved using a Hichrom KR 100, 250 x 4.6 mm C(18) with a mobile phase composition potassium dihydrogen phosphate buffer (0.01 m, pH 6.5):acetonitrile:methanol (40:50:10, v/v/v). The flow-rate of the mobile phase was set at 1 mL/min. The column eluate was monitored by fluorescence detector set at an excitation wavelength of 247 nm and emission wavelength of 367 nm. Linear relationships (r(2) > 0.99) were observed between the peak area ratio rosiglitazone to IS vs rosiglitazone concentrations across the concentration range 5-1000 ng/mL. The intra-run precision (%RSD) and accuracy (%Dev) in the measurement of rosiglitazone were <+/-10.69 and <-12.35%, respectively across the QC levels (50-1000 ng/mL). The extraction efficiency was >80% for both rosiglitazone and IS from human plasma. The lower limit of quantitation of the assay was 5 ng/mL. In summary, the methodology for rosiglitazone measurement in plasma was simple, sensitive and employed a commercially available IS.     

Third body modeling in fretting using the combined finite-discrete element method

A new approach was developed for modeling the effect of the third body on fretting. This was accomplished using the combined finite-discrete element method (FDEM) in which the third body is analyzed as discrete elements while the first bodies are modeled using finite elements. This approach provides a link between large scale models which treat the mass of wear debris as a single or small number of bodies and small scale models which only study a control volume. The FDEM was used to analyze the behavior of third body particles between flat sliding surfaces. When the third body mass is composed of unconnected particles, it behaves as a Newtonian fluid, but this behavior ceases when the particles are connected into platelets. The FDEM was also used to study the behavior of third body particles inside a Hertzian line contact. As the number of particles and platelet size increase the load carried by the worn slip zone grows larger in relationship to the unworn stick zone.     

QUADRATURE METHODS FOR HIGHLY OSCILLATORY SINGULAR INTEGRALS

We address the evaluation of highly oscillatory integrals,with power-law and logarithmic singularities.Such problems arise in numerical methods in engineering.Notably,the evaluation of oscillatory integrals dominates the run-time for wave-enriched boundary integral formulations for wave scattering,and many of these exhibit singularities.We show that the asymptotic behaviour of the integral depends on the integrand and its derivatives at the singular point of the integrand,the stationary points and the endpoints of the integral.A truncated asymptotic expansion achieves an error that decays faster for increasing frequency.Based on the asymptotic analysis,a Filon-type method is constructed to approximate the integral.Unlike an asymptotic expansion,the Filon method achieves high accuracy for both small and large frequency.Complex-valued quadrature involves interpolation at the zeros of polynomials orthogonal to a complex weight function.Numerical results indicate that the complex-valued Gaussian quadrature achieves the highest accuracy when the three methods are compared.However,while it achieves higher accuracy for the same number of function evaluations,it requires signi cant additional cost of computation of orthogonal polynomials and their zeros.     

Optimization of Biocompatibility for a Hydrophilic Biological Molecule Encapsulation System

Despite considerable advances in recent years, challenges in delivery and storage of biological drugs persist and may delay or prohibit their clinical application. Though nanoparticle-based approaches for small molecule drug encapsulation are mature, encapsulation of proteins remains problematic due to destabilization of the protein. Reverse micelles composed of decylmonoacyl glycerol (10MAG) and lauryldimethylamino-N-oxide (LDAO) in low-viscosity alkanes have been shown to preserve the structure and stability of a wide range of biological macromolecules. Here, we present a first step on developing this system as a future platform for storage and delivery of biological drugs by replacing the non-biocompatible alkane solvent with solvents currently used in small molecule delivery systems. Using a novel screening approach, we performed a comprehensive evaluation of the 10MAG/LDAO system using two preparation methods across seven biocompatible solvents with analysis of toxicity and encapsulation efficiency for each solvent. By using an inexpensive hydrophilic small molecule to test a wide range of conditions, we identify optimal solvent properties for further development. We validate the predictions from this screen with preliminary protein encapsulation tests. The insight provided lays the foundation for further development of this system toward long-term room-temperature storage of biologics or toward water-in-oil-in-water biologic delivery systems.     

170 Mb/s multilevel transmission over 115 m standard step-index plastic optical fiber using an integrated optical receiver

An optical receiver having a high linearity is used for multilevel communication over standard step plastic optical fiber. A large-diameter photodiode with an antireflection coating optimized for red light was integrated. These features enable the used optical receiver to be a promising plastic optical fiber receiver. An error free (< 10⁻⁸) 170 Mb/s data rate over 115 m standard PMMA step-index plastic optical fiber is achieved with four-level and eight-level pulse amplitude modulation.     

The use of Herschel–Quincke tubes to improve the efficiency of lined ducts

The scattering matrix results of Herschel–Quincke (HQ) resonators installed in combination with an acoustic liner (HQ-Liners) are presented in this paper. This approach aims at controlling both tonal and broadband noise to improve the liner efficiency. It uses circumferential arrays of Herschel–Quincke tubes on a main duct in a serial association with a locally reacting liner of known impedance. Results for the scattering matrix of this system are deduced from an analytical model and compared with experimental and numerical data showing a good agreement. Analysis of the scattering matrix coefficients points out the modal conversion properties that depend on the number of HQ tubes along the circumference. Results of the transmission loss show that the choice of an optimal HQ configuration with respect to the liner properties can substantially improve the liner efficiency.     

The Electron-Hole plasma in CdS between 5 K and room temperature

We investigate and analyze the time-integrated nonlinear optical behaviour of an electronhole plasma in CdS as a function of the temperature and the exciting intensity. The competition between the temperature dependences of the absorption tail and of the manyparticle effects leads with increasing temperature around 160 K to a turn-over from a red- to a blue-shift of the absorption edge under excitation. The frequency dependence of the absorption for high plasma concentrations is in good agreement with theoretical calculations.     

High pressure photoluminescence and resonant Raman study of GaAs

Photoluminescence and resonant Raman scattering have been studied in GaAs under hydrostatic stress up to 72 kbar. From the pressure dependence of the photoluminescence intensity the pressure coefficient of the X₆ conduction band minimum in GaAs has been determined. The pressure dependence of zone edge phonon energies in GaAs is also reported.     

On <Emphasis Type="Italic">g</Emphasis>-measures in symbolic dynamics

Finitary Markov processes are described in G. Morvai and B. Weiss, Prediction for discrete time series, Probability Theory and Related Fields 132 (2005), 1–12. The transition functions of finitary Markov processes are residually locally constant g-functions that can be extended by continuity to their maximal domain of definition. The study of their associated symbolic dynamics leads one to the D-shifts as introduced in W. Krieger, On g-functions for subshifts, Institute of Mathematical Statistics Lecture Notes-Monograph Series, Vol. 48, Dynamics & Stochastics, arXiv:math.DS/0608259, (2006), 306–316, We study the phenomena that can arise in residually locally constant and locally constant maximally defined g-functions on D-shifts, Markov shifts and synchronizing systems with respect to future measures and g-measures     

Frequency-domain birefringence measurement of biological binding to magnetic nanoparticles

Optical detection of the frequency-dependent magnetic relaxation signal is used to monitor the binding of biological molecules to magnetic nanoparticles in a ferrofluid. Biological binding reactions cause changes in the magnetic relaxation signal due to an increase in the average hydrodynamic diameter of the nanoparticles. To allow the relaxation signal to be detected in dilute ferrofluids, measurements are made using a balanced photodetector, resulting in a 25 μV/√Hz noise floor, within 50% of the theoretical limit imposed by photon shot noise. Measurements of a ferrofluid composed of magnetite nanoparticles coated with anti-IgG antibodies show that the average hydrodynamic diameter increases from 115.2 to 125.4 nm after reaction with IgG.